Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACNSN2_RS12750 Genome accession   NZ_CP183075
Coordinates   2730212..2731888 (+) Length   558 a.a.
NCBI ID   WP_418607617.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. US3C1013     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2725212..2736888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNSN2_RS12730 (ACNSN2_12730) - 2726474..2726923 (+) 450 WP_010391865.1 pilin -
  ACNSN2_RS12735 (ACNSN2_12735) pilC 2727055..2728284 (+) 1230 WP_418607612.1 type II secretion system F family protein Machinery gene
  ACNSN2_RS12740 (ACNSN2_12740) pilD 2728534..2729445 (+) 912 WP_418607614.1 prepilin peptidase Machinery gene
  ACNSN2_RS12745 (ACNSN2_12745) coaE 2729448..2730077 (+) 630 WP_418607615.1 dephospho-CoA kinase -
  ACNSN2_RS12750 (ACNSN2_12750) pilB 2730212..2731888 (+) 1677 WP_418607617.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNSN2_RS12755 (ACNSN2_12755) yacG 2731954..2732184 (+) 231 WP_010391853.1 DNA gyrase inhibitor YacG -
  ACNSN2_RS12760 (ACNSN2_12760) - 2732244..2732732 (-) 489 WP_418607619.1 Spy/CpxP family protein refolding chaperone -
  ACNSN2_RS12765 (ACNSN2_12765) - 2732899..2733588 (+) 690 WP_075169871.1 response regulator -
  ACNSN2_RS12770 (ACNSN2_12770) - 2733566..2734981 (+) 1416 WP_418607622.1 ATP-binding protein -
  ACNSN2_RS12775 (ACNSN2_12775) - 2734985..2735932 (+) 948 WP_418607624.1 alpha/beta fold hydrolase -
  ACNSN2_RS12780 (ACNSN2_12780) - 2735905..2736774 (-) 870 WP_082360146.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62309.59 Da        Isoelectric Point: 5.7551

>NTDB_id=1103987 ACNSN2_RS12750 WP_418607617.1 2730212..2731888(+) (pilB) [Pseudoalteromonas sp. US3C1013]
MNINSPLLRKFITLGRVDTDTVKARQQDFASTAELISKCGKIDSNDLAEQCIDLFRVPFFDLKDFNVAQIPTELVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYLQLDHKIEQLLDAAGSLNLSEDEFKEFAELDSEP
QKDHTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLASKLSARIKVMSRLDI
AEKRKPQDGRIKLKVTERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKELYLDALNQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCSKCKTPEQLPREELMRQGFSEQQIQDMVL
YAPKGCENCTDGFKGRVGIYEVMKVTPEIAQIIMRGGNSLEIAEVSLKAGFNNIRLSGLRKAAEGVTSLAEINRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1103987 ACNSN2_RS12750 WP_418607617.1 2730212..2731888(+) (pilB) [Pseudoalteromonas sp. US3C1013]
ATGAATATTAACTCACCGTTACTAAGAAAATTTATTACCCTCGGCCGGGTTGATACCGATACCGTAAAAGCCAGGCAGCA
AGACTTTGCGTCAACCGCAGAACTTATTTCTAAATGCGGAAAAATAGATAGCAACGATTTAGCTGAGCAATGTATTGATT
TGTTTAGGGTGCCTTTTTTTGATTTAAAAGATTTTAATGTTGCACAAATTCCTACCGAGCTAGTTAAAGAAAAGCTGATC
CGCAAGCATCATATTTTACCTTTGGTACAAAAAGGCAGGAAAGTATATATTGCCGCTTCAGATCCTACCGATTACGGCGC
TTTTGAAAACTTTGAATTTAGCACCGGTTTATCGTGTGAAGTGGTAGTAGTTGACTACCTACAGCTTGACCATAAAATTG
AGCAACTACTGGATGCTGCAGGTAGCTTAAATTTAAGTGAGGATGAATTTAAAGAGTTTGCTGAACTAGATTCTGAGCCA
CAAAAAGATCATACGCCAAAAGACGATGACAAAGACGACGCCCCTATTATTGTCTACATTAATAAAATTTTAATGGACGC
AATAAAAAAAGGCGCTTCGGATCTTCACTTTGAACCTTATGAACATAAATATCGTATTCGCTTTCGCATCGACGGAATTT
TGCATGAGGTAGCCAGCCCGCCAAATACCTTAGCCAGTAAGCTATCTGCTCGTATAAAAGTTATGTCACGCCTTGATATC
GCTGAAAAACGTAAGCCACAAGATGGCCGTATAAAACTCAAAGTGACAGAACGTAAAAGTATTGATTTTCGTGTTAGTAC
TATGCCCACTTTGTGGGGCGAAAAAATTGTAATGCGTATCCTCGATTCATCAAGTGCCATGTTAGGTATTGATGTTTTAG
GCTATGAACCTGAGCAGAAAGAGCTCTACCTCGATGCACTAAATCAACCTCAGGGGATGATTTTAGTTACTGGCCCAACT
GGCTCGGGTAAAACCGTCTCCCTGTATACGGGCTTAAATATACTAAATCAGCCCGAGCGAAATATTAGTACCGCCGAAGA
TCCGGTAGAAATTAACCTCGAAGGGATTAACCAAGTACAAATAAACACTAAAGCCGACATGACATTTGCTAACGCTTTGC
GGGCATTTTTACGCCAAGATCCCGATGTAGTAATGGTCGGTGAAATACGGGATTTAGAAACCGCTGAAATATCAATTAAA
GCGGCGCAAACCGGTCACTTAGTGTTATCTACATTACATACTAACTCAGCACCAGAAACCATTACCCGTTTGTTAAATAT
GGGCGTACCCGCGTATAATGTGGCCAGTTCAATCAGCTTGATAATCGCCCAGCGTTTAGCCCGAAGGTTATGCTCTAAAT
GTAAAACGCCAGAGCAATTACCCCGTGAAGAACTTATGCGCCAAGGCTTTAGTGAACAGCAAATACAGGATATGGTTTTA
TATGCCCCTAAAGGCTGTGAAAACTGTACAGATGGCTTCAAAGGCCGTGTTGGTATTTACGAAGTTATGAAAGTTACTCC
AGAAATTGCGCAAATTATTATGCGTGGGGGTAACTCACTAGAAATTGCCGAAGTATCACTTAAAGCAGGGTTTAATAACA
TACGCTTATCAGGTCTTCGAAAAGCAGCAGAGGGTGTTACCTCTCTGGCTGAGATAAACCGTGTTACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.963

96.774

0.561
  pilB Acinetobacter baylyi ADP1

58.416

90.502

0.529
  pilB Legionella pneumophila strain ERS1305867

55.599

91.219

0.507
  pilB Vibrio cholerae strain A1552

52.523

95.878

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

52.444

95.341

0.5
  pilB Vibrio campbellii strain DS40M4

50.749

95.699

0.486
  pilF Neisseria gonorrhoeae MS11

48.659

93.548

0.455

Multiple sequence alignment