Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACNSN2_RS12385 Genome accession   NZ_CP183075
Coordinates   2645077..2646213 (-) Length   378 a.a.
NCBI ID   WP_054201910.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. US3C1013     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2640077..2651213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNSN2_RS12355 (ACNSN2_12355) - 2640454..2641347 (+) 894 WP_010391581.1 hypothetical protein -
  ACNSN2_RS12360 (ACNSN2_12360) - 2641425..2641898 (+) 474 WP_010391582.1 hypothetical protein -
  ACNSN2_RS12365 (ACNSN2_12365) rsmE 2641969..2642703 (+) 735 WP_055012525.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACNSN2_RS12370 (ACNSN2_12370) gshB 2642770..2643720 (+) 951 WP_418607559.1 glutathione synthase -
  ACNSN2_RS12375 (ACNSN2_12375) - 2643848..2644405 (+) 558 WP_418607561.1 YqgE/AlgH family protein -
  ACNSN2_RS12380 (ACNSN2_12380) ruvX 2644423..2644872 (+) 450 WP_013465849.1 Holliday junction resolvase RuvX -
  ACNSN2_RS12385 (ACNSN2_12385) pilU 2645077..2646213 (-) 1137 WP_054201910.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNSN2_RS12390 (ACNSN2_12390) pilT 2646223..2647263 (-) 1041 WP_010391590.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNSN2_RS12395 (ACNSN2_12395) - 2647266..2647979 (+) 714 WP_418607564.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNSN2_RS12400 (ACNSN2_12400) proC 2647997..2648818 (+) 822 WP_010391593.1 pyrroline-5-carboxylate reductase -
  ACNSN2_RS12405 (ACNSN2_12405) - 2648845..2649378 (+) 534 WP_008113334.1 YggT family protein -
  ACNSN2_RS12410 (ACNSN2_12410) - 2649437..2649880 (+) 444 WP_010391596.1 DUF4426 domain-containing protein -
  ACNSN2_RS12415 (ACNSN2_12415) - 2649883..2650482 (+) 600 WP_418607567.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41970.00 Da        Isoelectric Point: 6.2366

>NTDB_id=1103983 ACNSN2_RS12385 WP_054201910.1 2645077..2646213(-) (pilU) [Pseudoalteromonas sp. US3C1013]
MTLSLNHFLQIMIDKKGSDLFVSSQLPISAKINGELIPLNDDKLTDEQALELVESAMSEKQKAEFHSTKECNFAIATSEG
RFRISAFWQRDRAGMVIRRIVTQIPDVKELGLPSVLTDVIMSKRGLVLFVGGTGTGKSTSLAALVGYRNRHQRGHILTIE
DPIEFVHEHQKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLIPTSDGEGRAAAIEVLLNTPMVSELIKNGNIGGIKETMAKSTEMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKGNIT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1103983 ACNSN2_RS12385 WP_054201910.1 2645077..2646213(-) (pilU) [Pseudoalteromonas sp. US3C1013]
ATGACTTTATCTCTAAATCACTTTTTACAAATAATGATCGACAAAAAAGGCTCTGATCTATTTGTATCAAGCCAGCTGCC
TATTAGCGCTAAAATTAATGGCGAGCTGATACCTCTTAATGATGACAAACTAACCGATGAGCAAGCGCTGGAATTAGTTG
AATCAGCGATGAGTGAAAAGCAAAAGGCAGAGTTTCATAGCACCAAAGAGTGTAACTTTGCAATTGCAACCAGCGAAGGG
CGCTTTCGTATTTCGGCTTTTTGGCAACGTGACAGAGCAGGGATGGTTATTCGCCGGATTGTGACCCAAATACCTGATGT
AAAAGAGCTGGGATTGCCCTCAGTACTTACTGATGTGATTATGTCAAAGCGAGGCCTGGTATTATTTGTAGGTGGTACGG
GAACCGGTAAATCAACATCACTGGCAGCACTGGTTGGTTATAGAAATCGCCATCAGCGCGGGCATATTCTAACCATAGAA
GATCCAATTGAATTTGTGCATGAGCACCAAAAAAGTATTATTACCCAACGTGAAGTGGGCTTAGATACAGAAAGCTTTGA
GTCAGCACTAAAAAGCTCTTTACGCCAAGCGCCAGATGTTATTTTGATTGGTGAAATTCGATCGCAAGAAACTATGGAGT
ATGCGTTAAGCTTTGCAGAAACAGGTCATTTATGTGTTGCCACCTTACATGCTAACAATGCTAACCAAGCTATTGATCGT
ATTATGCACTTAGTGCCAAAAGAGAAGCACGATAAGCTTAAATATGATTTAGCATTAAACTTACGCGCTATTATTGCCCA
GCAGCTTATCCCTACCTCTGATGGTGAGGGACGTGCTGCTGCTATAGAGGTATTATTGAATACGCCAATGGTGAGTGAGC
TGATTAAAAACGGTAACATTGGTGGTATTAAAGAAACTATGGCCAAATCAACTGAAATGGGCATGCAGACCTTTGACCAA
GCGCTGTTTGAGCTTTACAGACAGCAACGGATTAACTATGCCGATGCACTTCATCATGCAGACTCGCCAAATGATTTACG
TTTAATGATAAAACTACGTAATAACGAGCAGCAAGGGGCAGGCTTTTTGCAAGGTGTAACCATTGATGGCCTTGATGATA
AAGGTAATATTACCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

57.029

99.735

0.569
  pilU Pseudomonas stutzeri DSM 10701

57.333

99.206

0.569
  pilU Vibrio cholerae strain A1552

56.676

97.09

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.389

95.238

0.394

Multiple sequence alignment