Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACNR0F_RS06495 Genome accession   NZ_CP183037
Coordinates   1192335..1194020 (-) Length   561 a.a.
NCBI ID   WP_003787777.1    Uniprot ID   F5S8J8
Organism   Kingella kingae strain URMC_2412A945     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1187335..1199020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNR0F_RS06465 (ACNR0F_06465) - 1187496..1188116 (-) 621 WP_003790910.1 L-threonylcarbamoyladenylate synthase -
  ACNR0F_RS06470 (ACNR0F_06470) pncC 1188126..1188602 (-) 477 WP_003787789.1 nicotinamide-nucleotide amidase -
  ACNR0F_RS06475 (ACNR0F_06475) zapD 1188622..1189383 (-) 762 WP_003790912.1 cell division protein ZapD -
  ACNR0F_RS06480 (ACNR0F_06480) coaE 1189498..1190106 (-) 609 WP_003787784.1 dephospho-CoA kinase -
  ACNR0F_RS06485 (ACNR0F_06485) pilD 1190108..1191016 (-) 909 WP_032828171.1 prepilin peptidase Machinery gene
  ACNR0F_RS06490 (ACNR0F_06490) - 1191019..1192288 (-) 1270 Protein_1270 type II secretion system F family protein -
  ACNR0F_RS06495 (ACNR0F_06495) pilF 1192335..1194020 (-) 1686 WP_003787777.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNR0F_RS06500 (ACNR0F_06500) lysA 1194206..1195432 (-) 1227 WP_032134244.1 diaminopimelate decarboxylase -
  ACNR0F_RS06505 (ACNR0F_06505) lptM 1195404..1195598 (-) 195 WP_003790918.1 LPS translocon maturation chaperone LptM -
  ACNR0F_RS06510 (ACNR0F_06510) cyaY 1195689..1196012 (+) 324 WP_099046165.1 iron donor protein CyaY -
  ACNR0F_RS06515 (ACNR0F_06515) pgeF 1196162..1196941 (+) 780 WP_243763643.1 peptidoglycan editing factor PgeF -
  ACNR0F_RS06520 (ACNR0F_06520) - 1196994..1198619 (-) 1626 WP_038329106.1 ABC-F family ATPase -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62226.13 Da        Isoelectric Point: 5.1186

>NTDB_id=1103714 ACNR0F_RS06495 WP_003787777.1 1192335..1194020(-) (pilF) [Kingella kingae strain URMC_2412A945]
MSAGLLRILVQKKLVPQENLAKYQEAIKADKPILASIFADKLITPEDLAELCATMFNYPMLDLNFFPRSKIVPDILDESQ
MMELRCIPLFKRGKKLYLAVSDPTNIQNLQKVVFSSGLAIDLVIVRDDQLNTVLEWFGQSNSSLLKEMGMDSSLQEAQAQ
TGMIVDGEEEEDGPVARFIQKILHDAVTSGASDIHFEFYEFMARVRFRTDGQLREIVQPPLALRSQLASRIKVMAKLDIS
EKRVPQDGRIQLQFSKNTKAIDFRVNTMPCLFGEKVVMRILNSDAASLNIDQLGFEDFQKKLIMDAIYRPYGMVLVTGPT
GSGKTVSLYTCLNILNTDDVNISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDIIMIGEIRDLGTADIAIK
AAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASAVNLIMAQRLARRLCSACKAPMERPPENVLRDAGFTDEDLAKDW
TMYRAVGCDACKGKGYKGRAGLYEVMPMTEKMKAVIMKGGTEVDIANIAYEEGLCDLRRSGLLKVMQGVTTLEEVIATTN
E

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1103714 ACNR0F_RS06495 WP_003787777.1 1192335..1194020(-) (pilF) [Kingella kingae strain URMC_2412A945]
ATGAGCGCAGGTTTATTGCGAATTTTGGTACAGAAAAAACTCGTACCACAAGAGAATTTAGCCAAATATCAAGAAGCGAT
TAAAGCCGATAAACCGATTTTGGCTTCCATTTTTGCTGACAAGCTGATTACACCAGAAGACTTGGCAGAGCTGTGTGCCA
CCATGTTTAACTATCCGATGTTGGATTTAAACTTCTTCCCACGCAGCAAAATCGTCCCTGATATTTTGGATGAATCGCAA
ATGATGGAATTGCGCTGCATTCCATTGTTTAAGCGTGGTAAAAAATTGTATTTGGCGGTGTCTGACCCAACCAATATCCA
AAATTTGCAAAAAGTCGTGTTCAGCAGCGGCTTGGCGATTGATTTAGTGATTGTGCGCGATGATCAATTAAATACCGTGT
TGGAATGGTTTGGTCAAAGCAATAGCAGCCTGCTTAAAGAAATGGGCATGGACAGCAGCCTGCAAGAAGCACAAGCCCAA
ACAGGCATGATTGTGGATGGCGAAGAAGAAGAGGACGGCCCTGTTGCCCGTTTCATTCAAAAAATTCTGCACGATGCCGT
GACATCGGGTGCGTCCGATATTCACTTTGAGTTCTATGAATTTATGGCGCGTGTGCGTTTCCGTACAGACGGTCAGTTGC
GCGAAATCGTACAGCCACCATTGGCATTGCGTAGCCAGCTGGCATCGCGCATCAAGGTAATGGCGAAACTGGATATTTCT
GAAAAGCGCGTCCCACAAGACGGACGTATTCAGTTGCAATTTTCTAAAAATACCAAAGCGATTGACTTTCGTGTGAACAC
CATGCCGTGTTTGTTTGGCGAAAAAGTCGTGATGCGTATTTTGAACTCCGATGCCGCCAGCTTGAACATTGACCAACTGG
GTTTTGAAGACTTCCAGAAAAAATTGATTATGGACGCGATTTATCGTCCATACGGCATGGTGCTGGTAACAGGGCCTACG
GGCTCGGGTAAAACCGTATCGCTTTACACTTGCTTAAACATTTTGAATACCGATGACGTAAACATCTCCACCGCGGAAGA
CCCTGCGGAGATTAACTTACCAGGCATCAATCAAGTAAACGTAAACGACAAACAAGGCTTAACTTTTGAAGCTGCGCTAA
AAGCATTCTTGCGTCAAGACCCAGATATCATCATGATTGGTGAGATTCGTGACTTGGGTACAGCCGATATTGCGATTAAA
GCCGCACAAACAGGTCACATGGTGTTCTCAACCTTGCACACCAACAATGCCCCAGCCACCTTGTCGCGTATGTTGAACAT
GGGTGTTGCGCCGTTTAATATTGCATCGGCAGTGAACTTGATTATGGCGCAGCGTTTGGCACGTCGTTTGTGCAGCGCGT
GTAAAGCCCCAATGGAACGTCCGCCTGAAAATGTATTGCGCGATGCAGGTTTTACTGACGAAGATTTGGCAAAAGATTGG
ACAATGTATCGTGCCGTAGGTTGTGATGCGTGTAAAGGCAAAGGCTACAAAGGTCGTGCAGGCTTGTACGAAGTGATGCC
GATGACGGAAAAAATGAAAGCCGTTATCATGAAAGGCGGTACAGAAGTGGATATTGCTAACATTGCCTATGAAGAAGGTT
TGTGCGATTTGCGCCGTTCTGGCTTGCTCAAAGTGATGCAAGGTGTAACCACTTTGGAAGAAGTGATTGCGACAACCAAC
GAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(181-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5S8J8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.271

100

0.683
  pilB Acinetobacter baumannii D1279779

52.91

100

0.535
  pilB Acinetobacter baylyi ADP1

51.865

100

0.52
  pilB Legionella pneumophila strain ERS1305867

47.695

100

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.455
  pilB Vibrio campbellii strain DS40M4

45.421

95.365

0.433
  pilB Vibrio cholerae strain A1552

46.318

91.979

0.426
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.808

92.692

0.369

Multiple sequence alignment