Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   ACNSPL_RS29520 Genome accession   NZ_CP183002
Coordinates   5939865..5940908 (-) Length   347 a.a.
NCBI ID   WP_223295889.1    Uniprot ID   -
Organism   Klebsiella pneumoniae strain RKI 380DKa     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5934865..5945908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNSPL_RS29485 (ACNSPL_29470) - 5935153..5935476 (-) 324 WP_418287332.1 hypothetical protein -
  ACNSPL_RS29490 (ACNSPL_29475) - 5935529..5935705 (-) 177 WP_017644226.1 BOW99_gp33 family protein -
  ACNSPL_RS29495 (ACNSPL_29480) - 5935770..5936498 (-) 729 WP_001004151.1 phage antirepressor protein -
  ACNSPL_RS29500 (ACNSPL_29485) - 5936511..5936750 (-) 240 WP_000217789.1 helix-turn-helix domain-containing protein -
  ACNSPL_RS29505 (ACNSPL_29490) - 5936932..5937675 (+) 744 WP_001260003.1 XRE family transcriptional regulator -
  ACNSPL_RS29510 (ACNSPL_29495) - 5937692..5938219 (+) 528 WP_000734160.1 hypothetical protein -
  ACNSPL_RS29515 (ACNSPL_29500) - 5938343..5939788 (+) 1446 WP_000633502.1 recombinase family protein -
  ACNSPL_RS29520 (ACNSPL_29505) comYB 5939865..5940908 (-) 1044 WP_223295889.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACNSPL_RS29525 (ACNSPL_29510) comYA 5940802..5941773 (-) 972 WP_000250805.1 competence type IV pilus ATPase ComGA Machinery gene
  ACNSPL_RS29530 (ACNSPL_29515) - 5941946..5942311 (-) 366 WP_000285373.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39936.65 Da        Isoelectric Point: 9.9002

>NTDB_id=1103453 ACNSPL_RS29520 WP_223295889.1 5939865..5940908(-) (comYB) [Klebsiella pneumoniae strain RKI 380DKa]
MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDSMNKALLEGKDLSKML
GELGFSDTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGLRNYLMPQLGENNFATR
LITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTIPLVGSYVKLYLTAYYAREWGNLLSQGIELDQIVKVMQN
QKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVI
FIFVALIIVMIYAAMLLPMYQNMEILS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1103453 ACNSPL_RS29520 WP_223295889.1 5939865..5940908(-) (comYB) [Klebsiella pneumoniae strain RKI 380DKa]
ATAGACAAGTGGATATCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAA
ACAACGGAAAGTAGTCCAACTTTTTAATAATCTTTTTGCTAGTGGTTTTTCTTTAACTGATATGGTCACCTTCTTAAAGA
GGAGTAAGTTATTGTCTGATTGTTATACAGATAGTATGAATAAGGCATTATTAGAGGGAAAAGATTTATCAAAAATGTTA
GGAGAGTTAGGTTTTTCTGACACTGTTATCACACAGGTTGCATTAGCTGATTTGCATGGTAACATTTCAAGGAGCCTACT
AAAGATTGAGTCTTATTTAGCTAATCTTTTGTTAGTTAGAAAAAAAGTAATAGAAGTAGCTACTTACCCATTGATATTAT
TGTCTTTTCTGGTGCTAATTATGATTGGCCTTAGGAATTATTTAATGCCCCAATTAGGAGAAAATAATTTTGCAACTAGA
CTGATTACAAATGTGCCGAATATTTTCTTATTACTTTTAGCAGTTGTACTTATTTTTAGTTTAATATTTTATATTATTCA
AAAGCGATTGTCGCGCATTAAAGTAGCTTGTTTTTTAACAACAATTCCCTTAGTTGGATCATATGTTAAGCTTTATTTAA
CTGCTTACTATGCCCGTGAGTGGGGAAATTTATTAAGTCAAGGTATTGAATTGGACCAAATTGTTAAAGTAATGCAAAAT
CAGAAATCCAAACTTTTTAGGGAAATAGGATATGACATGGAAGAAGGTTTTCTATCAGGTAAAGCATTTCACCAAAAAGT
ATTAGACTATCCGTTTTTCTTAACTGAGCTTAGTTTAATGATTGAATATGGCCAAGTTAAGGCGAAATTAGGAACAGAGT
TAGATATATATGCTGATGAGAAGTGGGAGGATTTTTTTACAAAATTAGCTAGAGCGACCCAGTTAATCCAACCCGTTATT
TTTATTTTTGTAGCTCTTATCATTGTTATGATTTATGCAGCAATGCTGTTACCAATGTATCAAAATATGGAGATATTATC
ATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(34-150)

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

62.17

98.271

0.611
  comYB Streptococcus mutans UA159

61.877

98.271

0.608
  comYB Streptococcus gordonii str. Challis substr. CH1

56.305

98.271

0.553
  comGB/cglB Streptococcus mitis NCTC 12261

55.224

96.542

0.533
  comGB/cglB Streptococcus mitis SK321

54.627

96.542

0.527
  comGB/cglB Streptococcus pneumoniae Rx1

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae D39

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae R6

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae TIGR4

53.731

96.542

0.519
  comGB Lactococcus lactis subsp. cremoris KW2

50.148

97.118

0.487

Multiple sequence alignment