Detailed information NTG0110298

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACAK31_RS01540
• Genome accession: NZ_AP031581
• Coordinates: 329825..330883 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain JUNP496
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 324825..335883

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACAK31_RS01525 (JUNP496_0310)	-	325931..326263 (+)	333	WP_000169602.1	hypothetical protein	-
ACAK31_RS01530 (JUNP496_0311)	-	326266..327090 (+)	825	WP_096877324.1	putative RNA methyltransferase	-
ACAK31_RS01535 (JUNP496_0312)	ponA	327108..329663 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
ACAK31_RS01540 (JUNP496_0313)	pilM	329825..330883 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
ACAK31_RS01545 (JUNP496_0314)	pilN	330883..331524 (+)	642	WP_000201228.1	PilN domain-containing protein	Machinery gene
ACAK31_RS01550 (JUNP496_0315)	pilO	331521..332261 (+)	741	WP_000076106.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACAK31_RS01555 (JUNP496_0316)	pilP	332272..332799 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
ACAK31_RS01560 (JUNP496_0317)	pilQ	332862..335027 (+)	2166	WP_001017034.1	type IV pilus secretin PilQ	Machinery gene
ACAK31_RS01565 (JUNP496_0318)	aroK	335039..335608 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=110298 ACAK31_RS01540 WP_002014066.1 329825..330883(+) (pilM) [Acinetobacter baumannii strain JUNP496]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=110298 ACAK31_RS01540 WP_002014066.1 329825..330883(+) (pilM) [Acinetobacter baumannii strain JUNP496]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTCTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACAACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423