Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACAK31_RS01550 Genome accession   NZ_AP031581
Coordinates   331521..332261 (+) Length   246 a.a.
NCBI ID   WP_000076106.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain JUNP496     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 326521..337261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAK31_RS01535 (JUNP496_0312) ponA 327108..329663 (-) 2556 WP_000736677.1 penicillin-binding protein PBP1a -
  ACAK31_RS01540 (JUNP496_0313) pilM 329825..330883 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  ACAK31_RS01545 (JUNP496_0314) pilN 330883..331524 (+) 642 WP_000201228.1 PilN domain-containing protein Machinery gene
  ACAK31_RS01550 (JUNP496_0315) pilO 331521..332261 (+) 741 WP_000076106.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACAK31_RS01555 (JUNP496_0316) pilP 332272..332799 (+) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  ACAK31_RS01560 (JUNP496_0317) pilQ 332862..335027 (+) 2166 WP_001017034.1 type IV pilus secretin PilQ Machinery gene
  ACAK31_RS01565 (JUNP496_0318) aroK 335039..335608 (+) 570 WP_000643712.1 shikimate kinase AroK -
  ACAK31_RS01570 (JUNP496_0319) aroB 335637..336719 (+) 1083 WP_001193997.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27805.56 Da        Isoelectric Point: 4.8357

>NTDB_id=110300 ACAK31_RS01550 WP_000076106.1 331521..332261(+) (pilO) [Acinetobacter baumannii strain JUNP496]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWVFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=110300 ACAK31_RS01550 WP_000076106.1 331521..332261(+) (pilO) [Acinetobacter baumannii strain JUNP496]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGGTTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAGCTTCAAGAAAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCCCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

99.593

100

0.996
  comO Acinetobacter baylyi ADP1

69.008

98.374

0.679

Multiple sequence alignment