Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACM67C_RS02840 Genome accession   NZ_CP181246
Coordinates   574210..576264 (-) Length   684 a.a.
NCBI ID   WP_066077383.1    Uniprot ID   A0A378UEB4
Organism   Bergeriella denitrificans strain DSM 17675     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 569210..581264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM67C_RS02825 (ACM67C_02825) - 572040..572291 (-) 252 WP_066077368.1 DUF1289 domain-containing protein -
  ACM67C_RS02830 (ACM67C_02830) aroB 572389..573474 (-) 1086 WP_066077372.1 3-dehydroquinate synthase -
  ACM67C_RS02835 (ACM67C_02835) - 573571..574098 (-) 528 WP_066077375.1 shikimate kinase -
  ACM67C_RS02840 (ACM67C_02840) pilQ 574210..576264 (-) 2055 WP_066077383.1 type IV pilus secretin PilQ Machinery gene
  ACM67C_RS02845 (ACM67C_02845) - 576320..576862 (-) 543 WP_115225345.1 pilus assembly protein PilP -
  ACM67C_RS02850 (ACM67C_02850) - 576873..577502 (-) 630 WP_066077386.1 type 4a pilus biogenesis protein PilO -
  ACM67C_RS02855 (ACM67C_02855) pilN 577502..578104 (-) 603 WP_066077389.1 PilN domain-containing protein Machinery gene
  ACM67C_RS02860 (ACM67C_02860) pilM 578105..579211 (-) 1107 WP_066077392.1 type IV pilus assembly protein PilM -

Sequence

Protein


Download         Length: 684 a.a.        Molecular weight: 74663.42 Da        Isoelectric Point: 8.7806

>NTDB_id=1099533 ACM67C_RS02840 WP_066077383.1 574210..576264(-) (pilQ) [Bergeriella denitrificans strain DSM 17675]
MNSKHVKNLLAGLGMAAAFQTAFAGNITDISVSSLPDNQKIIKIRFDQDATLPSGFVTGTPARIALDFVGTQVRLAQSVF
EYADPLLDQITTAQNNNRARIVLGLHRAGQYTAEIRDDEVWIFVSEATDRNNLSIIADPVPHAAPAAHIDFRKGAYGSGV
VELAADGFNGRPDIKEQGDRVVITLKNHTLPTQAQRSLDVRDFDTPVRNITLKRIGNATRLTITNTGRWELNTRTSGNRF
YFEIRPKAADSAPAGLPSRSAKSFSGRRLSLDFQDIDVRTILQILAKESGLNIVASDSVRGKMTLSLKDVPWDQALDLVM
QARNLDMRRQGNIINIAPREELLAKDKAFLQAEKEIAELGPLYSQTFQFKYKNVEEFRKILRLDESGRSSSGDHRNTLLS
PRGSALIDPGTNTLIVTDTRNVIEKFRKLVDELDVAARQVMVEARIVEAEEGFSRDLGIKFGYAGSSSGGNTGWGNTWNG
GNTLTNNPNISLPVAAATSSVALVRSFSSAALGLELSASEAERKTKTISNPRVLTQDRKEASIESGQEIPYQESSSSGAT
SVTFKKAVLGLTVTPNITPDGQIIMTVKINRDTPVPCQTDGVNTLCISTKNLNTQAMVEDGGTLIIGGIYEETSGNTVNK
VPFLGDIPVVGNLFKSRARSEERKELLVFITPRIMDGQGNNLRY

Nucleotide


Download         Length: 2055 bp        

>NTDB_id=1099533 ACM67C_RS02840 WP_066077383.1 574210..576264(-) (pilQ) [Bergeriella denitrificans strain DSM 17675]
ATGAACAGCAAACACGTCAAAAACCTTCTGGCCGGATTGGGCATGGCCGCCGCTTTTCAGACGGCCTTTGCCGGCAACAT
TACCGACATCAGCGTCTCTTCCCTGCCCGACAACCAGAAAATCATCAAAATCCGTTTCGACCAAGACGCCACCCTGCCCA
GCGGCTTCGTGACCGGCACGCCGGCACGGATTGCCCTGGATTTCGTCGGCACGCAGGTACGTCTGGCACAATCCGTATTC
GAATACGCCGACCCCCTGCTCGACCAGATTACCACCGCGCAAAACAACAACCGCGCCCGCATCGTATTGGGGCTGCACCG
CGCCGGACAATACACCGCCGAAATCCGCGATGACGAAGTATGGATATTCGTCAGCGAAGCGACCGACCGCAACAACCTGT
CGATTATCGCCGACCCCGTGCCTCATGCCGCACCCGCCGCCCACATCGACTTCCGCAAAGGCGCATACGGCAGCGGCGTT
GTCGAGCTGGCCGCCGACGGCTTCAACGGCCGCCCGGACATCAAAGAGCAAGGCGACCGCGTCGTCATCACGCTGAAAAA
CCACACCCTGCCCACGCAGGCACAGCGCAGCTTGGATGTCCGCGACTTCGACACCCCCGTGCGCAACATCACCCTCAAGC
GCATCGGCAACGCCACCCGCCTCACCATCACCAATACCGGCCGCTGGGAGCTGAATACCCGCACCTCCGGCAACCGCTTC
TATTTTGAAATCCGCCCCAAAGCCGCCGACAGCGCCCCTGCCGGCCTGCCGTCGCGTTCGGCCAAATCATTCAGCGGACG
CAGATTGTCGCTGGATTTCCAAGACATCGACGTACGCACCATTCTGCAGATTCTGGCTAAAGAATCCGGCCTCAACATCG
TGGCCAGCGATTCGGTTCGCGGCAAAATGACCCTGTCGCTGAAAGACGTGCCCTGGGATCAGGCGCTGGATTTGGTGATG
CAGGCGCGCAATCTCGATATGCGCCGCCAGGGCAACATCATCAACATCGCCCCGCGCGAAGAGCTGCTGGCCAAAGACAA
AGCCTTTTTGCAAGCCGAAAAAGAGATTGCCGAGCTGGGGCCGCTGTATTCCCAAACCTTCCAGTTTAAATACAAAAACG
TGGAAGAGTTCCGCAAAATCCTGCGTTTGGACGAAAGCGGACGCAGCAGCAGCGGCGACCACCGCAACACCCTGCTCAGC
CCGCGCGGCAGCGCCTTAATCGACCCCGGCACCAACACCCTGATTGTCACCGACACGCGCAACGTCATTGAAAAATTCCG
CAAGCTGGTGGACGAGCTGGATGTTGCCGCGCGGCAGGTGATGGTGGAAGCGCGCATTGTCGAAGCCGAAGAAGGCTTCT
CACGCGATCTCGGCATCAAATTCGGCTATGCCGGCAGCAGCTCAGGCGGCAATACCGGTTGGGGCAACACCTGGAACGGC
GGCAATACCCTGACCAACAATCCCAACATCAGCCTGCCCGTTGCCGCCGCCACATCTTCCGTTGCGCTGGTACGCTCGTT
TTCATCGGCCGCGCTCGGGCTGGAGCTTTCCGCGTCCGAAGCCGAACGCAAAACCAAAACCATTTCCAACCCGCGCGTCC
TAACCCAAGACCGCAAAGAAGCCTCGATTGAATCCGGCCAGGAAATCCCCTACCAGGAATCCAGCTCCAGCGGCGCGACT
TCCGTCACCTTCAAAAAAGCCGTGCTCGGCCTGACCGTCACCCCCAACATCACGCCCGACGGCCAAATCATCATGACGGT
GAAAATCAACCGCGACACCCCCGTGCCCTGCCAAACCGACGGCGTCAACACCTTGTGTATCAGCACCAAAAACCTGAACA
CCCAAGCCATGGTGGAAGACGGCGGCACGCTCATCATCGGCGGCATCTACGAAGAAACCAGCGGCAATACCGTCAACAAA
GTGCCGTTTTTAGGCGATATTCCCGTTGTCGGCAACCTCTTCAAATCCCGCGCCCGCTCCGAAGAGCGCAAAGAGCTGCT
GGTCTTCATCACTCCCCGGATTATGGACGGACAAGGCAACAACTTACGCTATTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-118)

Secretin and TonB N terminus short domain (STN)

(292-338)

Bacterial type II and III secretion system protein (Secretin)

(519-676)

Bacterial type II/III secretion system short domain (Secretin_N)

(365-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378UEB4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

60.302

100

0.642
  pilQ Neisseria meningitidis MC58

65.082

79.971

0.52
  pilQ Neisseria meningitidis M1080

64.899

79.971

0.519
  comQ Acinetobacter baylyi ADP1

37.155

100

0.374

Multiple sequence alignment