Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACM0O0_RS01520 Genome accession   NZ_CP180689
Coordinates   314287..315066 (+) Length   259 a.a.
NCBI ID   WP_416040967.1    Uniprot ID   -
Organism   Acinetobacter lactucae isolate nanjing     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 309287..320066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM0O0_RS01505 ponA 309868..312429 (-) 2562 WP_416040965.1 penicillin-binding protein PBP1a -
  ACM0O0_RS01510 pilM 312591..313649 (+) 1059 WP_016143662.1 pilus assembly protein PilM Machinery gene
  ACM0O0_RS01515 pilN 313649..314290 (+) 642 WP_416040966.1 PilN domain-containing protein Machinery gene
  ACM0O0_RS01520 pilO 314287..315066 (+) 780 WP_416040967.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACM0O0_RS01525 pilP 315078..315605 (+) 528 WP_125738852.1 pilus assembly protein PilP Machinery gene
  ACM0O0_RS01530 pilQ 315668..317794 (+) 2127 WP_416040968.1 type IV pilus secretin PilQ Machinery gene
  ACM0O0_RS01535 aroK 317806..318375 (+) 570 WP_002116987.1 shikimate kinase AroK -
  ACM0O0_RS01540 aroB 318404..319486 (+) 1083 WP_416040969.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29318.27 Da        Isoelectric Point: 6.0348

>NTDB_id=1097444 ACM0O0_RS01520 WP_416040967.1 314287..315066(+) (pilO) [Acinetobacter lactucae isolate nanjing]
MSPHELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVTIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFIVEAAPVKDSKSDIPVLNYSIKAKTYRYMGAGENQNNNDPQGGNGETQA
NASDGHNVNIPSIKKSKRK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=1097444 ACM0O0_RS01520 WP_416040967.1 314287..315066(+) (pilO) [Acinetobacter lactucae isolate nanjing]
ATGAGTCCTCATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACTTTAGAAAAATTTCT
TCAGCAATTTAATACACTCGATATGAACAGCTATGGCAGTTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTATTGGCCTTGGGCTATTTTGTTACTATCCAGCCAAAGCTCCAAGCAATTGATAATGCTCAGGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGTAACTTGCAGCAATATCAGCTTCAACTTCAAGAAAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGAGTGAATTCTGGTCTGAAGTTTAAAAATATCCGTTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCG
ATTACGATTGAGGCAACAGGCGATTATCACGCTTTCGGTGCTTTCGTGAGCAGTATTGCGGCATTACCGCGTATTGTGAC
GATGCATGATTTTATTGTTGAAGCTGCTCCTGTTAAGGATAGTAAATCAGATATTCCTGTGCTTAATTATTCTATCAAAG
CAAAAACTTATCGCTATATGGGCGCTGGGGAAAACCAAAATAATAATGACCCACAAGGGGGAAATGGTGAGACGCAGGCC
AACGCCTCCGATGGTCATAATGTAAATATTCCTTCTATCAAGAAGTCAAAGCGAAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

89.286

86.486

0.772
  comO Acinetobacter baylyi ADP1

69.547

93.822

0.653

Multiple sequence alignment