Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMXYV_RS03780 Genome accession   NZ_CP180543
Coordinates   852740..854455 (-) Length   571 a.a.
NCBI ID   WP_415890441.1    Uniprot ID   -
Organism   Neptuniibacter sp. SY11_33     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 847740..859455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYV_RS03755 (ACMXYV_03755) - 848788..849747 (+) 960 WP_415890436.1 Nudix family hydrolase -
  ACMXYV_RS03760 (ACMXYV_03760) yacG 849725..849952 (-) 228 WP_415890437.1 DNA gyrase inhibitor YacG -
  ACMXYV_RS03765 (ACMXYV_03765) coaE 849952..850554 (-) 603 WP_415890438.1 dephospho-CoA kinase -
  ACMXYV_RS03770 (ACMXYV_03770) - 850558..851490 (-) 933 WP_415890439.1 prepilin peptidase -
  ACMXYV_RS03775 (ACMXYV_03775) pilC 851503..852723 (-) 1221 WP_415890440.1 type II secretion system F family protein Machinery gene
  ACMXYV_RS03780 (ACMXYV_03780) pilB 852740..854455 (-) 1716 WP_415890441.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYV_RS03785 (ACMXYV_03785) - 854704..855210 (+) 507 WP_415890442.1 pilin -
  ACMXYV_RS03790 (ACMXYV_03790) - 855401..855742 (+) 342 WP_415890443.1 hypothetical protein -
  ACMXYV_RS03795 (ACMXYV_03795) nadC 855829..856674 (-) 846 WP_415890444.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMXYV_RS03800 (ACMXYV_03800) ampD 856776..857321 (+) 546 WP_415890445.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACMXYV_RS03805 (ACMXYV_03805) - 857322..858029 (-) 708 WP_415890446.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63133.96 Da        Isoelectric Point: 4.7056

>NTDB_id=1096327 ACMXYV_RS03780 WP_415890441.1 852740..854455(-) (pilB) [Neptuniibacter sp. SY11_33]
MQPSQPLSGLAKRLVNEGVCSAEVAADASIQSREKQQTFISYVIEQRLVSAYRAAAAASEEFGMPLLDLDALDLNTLPQG
LINDSLISKHHALPLQKRDNRLFVAISDPTNIQALDEFKFQSGITTEAIIVEEDKLSRAIEAFLEGQDSALDELEDSDLD
NLEISDGTAPEQEEKESEEDATKDAPIVRFINKILLDSIKNGASDIHFEPYENSYRIRSRIDGILQEIAKPPSNLAARLS
ARLKVMSQMDISEKRVPQDGRIKMKISKKRAIDFRVNTLPTLWGEKIVLRILDPSSAKMGVEALGFADFQKELYLTTLHR
PQGMVLVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHVNAKVGLNFAEALRAFLRQDPDVVMVGEI
RDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLGRRLCEACKQPATLPEVTLLEEG
FLEEEITDLTLFEPNHDGCSKCNRGYKGRVGIYEMLPMSPEIAEVIMENGNSLDILRVAETQNFQSLRRSGLKKVAEGIT
SLEEMNRVIKT

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1096327 ACMXYV_RS03780 WP_415890441.1 852740..854455(-) (pilB) [Neptuniibacter sp. SY11_33]
TTGCAACCAAGTCAACCGTTAAGCGGGCTTGCCAAGCGATTAGTTAATGAAGGGGTATGTTCCGCAGAAGTTGCTGCTGA
TGCATCAATTCAATCAAGAGAAAAACAGCAAACCTTTATTTCCTATGTGATAGAGCAGCGCTTAGTAAGCGCTTATAGAG
CGGCAGCTGCAGCCTCTGAAGAATTTGGCATGCCATTGCTTGACTTGGACGCTCTAGATTTGAATACTTTGCCACAAGGT
CTAATTAACGACTCCCTAATATCCAAGCACCATGCCCTCCCCTTACAAAAGAGGGATAATCGTCTTTTTGTAGCCATCTC
TGATCCAACTAACATCCAAGCACTCGATGAATTTAAATTTCAAAGCGGGATAACTACTGAAGCCATTATTGTTGAGGAAG
ACAAACTATCAAGAGCGATTGAAGCCTTTCTTGAGGGCCAGGATTCAGCTCTAGATGAATTAGAAGACAGTGACCTAGAC
AATTTAGAAATTTCTGATGGAACCGCTCCCGAGCAAGAAGAGAAAGAAAGCGAAGAGGACGCAACTAAAGACGCGCCGAT
AGTCCGCTTTATCAATAAAATCCTTTTAGATTCAATTAAAAATGGCGCTTCAGATATTCACTTCGAACCTTATGAAAATA
GTTACAGAATCAGAAGCAGGATTGATGGGATACTGCAAGAAATAGCAAAACCTCCGTCAAATCTCGCTGCTAGGTTATCT
GCTCGCTTAAAGGTCATGTCACAAATGGATATCTCTGAAAAACGCGTTCCCCAAGACGGACGCATCAAAATGAAGATCTC
CAAAAAGCGCGCTATCGACTTCCGTGTAAATACTCTCCCTACCCTTTGGGGCGAAAAAATCGTACTTCGTATCCTCGACC
CTAGCAGCGCAAAAATGGGAGTTGAGGCCTTAGGCTTTGCAGACTTTCAGAAAGAACTTTACCTAACGACGCTTCACCGC
CCACAAGGCATGGTACTGGTAACAGGACCTACGGGTAGTGGTAAGACCGTTAGTCTTTATACCGGCCTCAACATTCTCAA
TACAGAAGAACGAAATATTTCCACTGCGGAAGACCCGGTAGAGATCAACCTAGAAGGAATTAATCAGGTTCACGTAAATG
CTAAAGTCGGCCTAAATTTTGCCGAGGCCCTACGTGCCTTTCTACGTCAGGACCCAGATGTTGTAATGGTTGGTGAGATT
CGAGATTTAGAAACAGCAGAGATAGCCATTAAAGCCGCTCAAACCGGCCATATGGTATTATCAACACTCCACACCAACAG
TGCACCCGAAACACTGACACGATTACGAAATATGGGTGTACCTGCGTTTAATATTGCAACATCAGTAAGCCTTATTATCG
CCCAACGCTTGGGCCGACGCCTTTGTGAAGCATGCAAGCAACCAGCCACTCTACCTGAAGTAACACTATTAGAAGAAGGT
TTTTTAGAAGAGGAAATAACAGATTTAACTCTTTTTGAACCTAATCATGATGGCTGTAGTAAATGCAATCGCGGCTATAA
AGGAAGGGTCGGTATCTATGAGATGCTTCCAATGTCACCAGAAATTGCAGAAGTAATTATGGAGAATGGCAACTCTCTAG
ATATACTAAGAGTAGCTGAAACACAAAACTTCCAAAGCCTAAGGCGCTCTGGCCTTAAAAAAGTTGCTGAAGGAATCACA
AGTCTTGAAGAGATGAACCGAGTCATCAAGACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.019

100

0.59
  pilB Acinetobacter baylyi ADP1

58.761

98.949

0.581
  pilB Legionella pneumophila strain ERS1305867

54.577

99.475

0.543
  pilB Vibrio cholerae strain A1552

55.598

90.718

0.504
  pilB Vibrio campbellii strain DS40M4

53.295

90.368

0.482
  pilF Neisseria gonorrhoeae MS11

48.754

98.424

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

53.216

89.842

0.478

Multiple sequence alignment