Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMXYI_RS02445 Genome accession   NZ_CP180481
Coordinates   511322..513022 (+) Length   566 a.a.
NCBI ID   WP_415771468.1    Uniprot ID   -
Organism   Pseudomonas sp. LB3P38     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 506322..518022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYI_RS02425 - 506605..509556 (+) 2952 WP_415771462.1 DUF748 domain-containing protein -
  ACMXYI_RS02430 - 509556..509852 (+) 297 WP_123367810.1 DUF2845 domain-containing protein -
  ACMXYI_RS02435 - 510025..510378 (-) 354 WP_415771464.1 BON domain-containing protein -
  ACMXYI_RS02440 - 510692..511096 (-) 405 WP_415771466.1 pilin -
  ACMXYI_RS02445 pilB 511322..513022 (+) 1701 WP_415771468.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYI_RS02450 pilC 513025..514242 (+) 1218 WP_415771470.1 type II secretion system F family protein Machinery gene
  ACMXYI_RS02455 pilD 514244..515116 (+) 873 WP_415771472.1 prepilin peptidase Machinery gene
  ACMXYI_RS02460 coaE 515113..515736 (+) 624 WP_309674604.1 dephospho-CoA kinase -
  ACMXYI_RS02465 yacG 515733..515933 (+) 201 WP_140681081.1 DNA gyrase inhibitor YacG -
  ACMXYI_RS02470 - 515937..516152 (-) 216 WP_033054332.1 hypothetical protein -
  ACMXYI_RS02475 - 516216..516905 (-) 690 WP_415771473.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62774.82 Da        Isoelectric Point: 5.9747

>NTDB_id=1096086 ACMXYI_RS02445 WP_415771468.1 511322..513022(+) (pilB) [Pseudomonas sp. LB3P38]
MNDIALSGLAKQLVLAELLTDKSAQQAYQQAQHNRIPLVSYLVQNKLVKSRQVAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLIRQHHALPLWRRGNKLFVGVSDPTNQQAINDIQFSTGLNTEAILVEDDKLSDAIEKFFDSHSTGLEDMADVDLDG
VDIESIDDNKQDSIGGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPIQLANRIASRL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEQDQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLGFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKKPIEIPREALLKEGFPE
ERIGSFTIYEPVGCDHCNNGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1096086 ACMXYI_RS02445 WP_415771468.1 511322..513022(+) (pilB) [Pseudomonas sp. LB3P38]
ATGAATGACATCGCCCTTAGTGGTCTGGCCAAGCAATTGGTCCTGGCCGAACTGCTCACTGACAAAAGCGCGCAACAGGC
GTATCAGCAAGCCCAACACAATCGAATCCCCCTGGTCAGTTACCTGGTTCAGAACAAACTGGTGAAAAGCCGCCAGGTCG
CCGAGATCGCGTCAGAACATTTCGGCATGGCACTGCTTGATCTCAACTGCCTGGACAAGGAAACCCAACCCAAGGGTCTG
GTCAGCGAGAAACTGATCCGCCAACACCACGCCCTGCCCCTCTGGCGACGCGGCAACAAGTTGTTCGTGGGCGTTTCCGA
CCCGACCAATCAGCAAGCCATCAATGACATCCAGTTCAGCACCGGGCTGAATACCGAAGCCATCCTCGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAATTCTTCGATAGCCATAGCACCGGCCTGGAAGACATGGCCGATGTCGACCTTGATGGT
GTGGACATCGAGTCGATCGATGACAACAAGCAGGATTCCATTGGCGGACAAGACGCCGACGACGCACCCGTGGTGCGCTT
CGTCCACAAGATGTTGCTCGACGCGATCAAGAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGCG
TGCGGATGCGCACCGACGGCATGCTGCGTGAAGTCGCCAAGCCGCCGATTCAACTGGCCAACCGCATCGCCTCGCGACTG
AAAGTCATGGCCAGTCTCGACATATCCGAGCGACGCAAACCACAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CACAAATGGGCATCGATGCCCTCGGTTACGAGCAGGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAGCCACAGGGG
ATGATTCTGGTGACAGGCCCCACCGGCTCGGGCAAGACCGTGTCGCTCTACACCGGTCTGAATATCCTCAACACCGTGGA
CATCAACATCTCCACCGCCGAAGACCCGGTAGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAGG
GACTGGGCTTTGCCCAGGCGCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTAATCATGGTTGGCGAGATTCGTGACCTC
GAAACCGCAGAAATAGCCATCAAGGCTGCCCAGACCGGGCACCTGGTGCTGTCGACCCTGCACACCAACAGCGCCGCCGA
AACCCTGACACGCCTGCACAACATGGGCATCCAGGGGTTCAACATTGCTACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAGCTATGCAGCCATTGCAAGAAACCCATCGAGATTCCCCGGGAGGCGCTGCTCAAGGAAGGCTTCCCCGAG
GAACGGATCGGCTCATTCACGATCTATGAGCCAGTCGGTTGCGATCACTGCAACAACGGTTACAAAGGGCGAGTAGGGAT
TTATGAAGTGGTGAAGAATACCCCGGACCTGCAACGGCTGATCATGGCCGAAGGCAACTCACTGGAAATCGATATCCAGA
TGCGTAAGGACGGTTTCAATGACCTGCGGACGTCGGGCCTGCTCAAGGCCATGCAAGGTATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.97

99.47

0.587
  pilB Acinetobacter baumannii D1279779

58.333

99.647

0.581
  pilB Legionella pneumophila strain ERS1305867

55.398

99.823

0.553
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.719

98.233

0.498
  pilF Neisseria gonorrhoeae MS11

49.911

99.47

0.496
  pilB Vibrio campbellii strain DS40M4

49.123

100

0.495
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.983

93.816

0.366

Multiple sequence alignment