Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACMYQ0_RS15350 Genome accession   NZ_CP180477
Coordinates   3314098..3315162 (-) Length   354 a.a.
NCBI ID   WP_136663373.1    Uniprot ID   A0A4T2A0L6
Organism   Pseudomonas leptonychotis strain CCM 8849     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3309098..3320162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMYQ0_RS15325 aroK 3309713..3310231 (-) 519 WP_136663369.1 shikimate kinase AroK -
  ACMYQ0_RS15330 pilQ 3310236..3312329 (-) 2094 WP_415755945.1 type IV pilus secretin PilQ Machinery gene
  ACMYQ0_RS15335 pilP 3312385..3312909 (-) 525 WP_415753683.1 type 4a pilus biogenesis lipoprotein PilP -
  ACMYQ0_RS15340 pilO 3312906..3313529 (-) 624 WP_415753684.1 type 4a pilus biogenesis protein PilO -
  ACMYQ0_RS15345 - 3313526..3314098 (-) 573 WP_415753685.1 PilN domain-containing protein -
  ACMYQ0_RS15350 pilM 3314098..3315162 (-) 1065 WP_136663373.1 pilus assembly protein PilM Machinery gene
  ACMYQ0_RS15355 - 3315347..3317761 (+) 2415 WP_415753686.1 penicillin-binding protein 1A -
  ACMYQ0_RS15360 - 3317855..3319123 (-) 1269 WP_136663375.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37869.32 Da        Isoelectric Point: 4.4612

>NTDB_id=1095990 ACMYQ0_RS15350 WP_136663373.1 3314098..3315162(-) (pilM) [Pseudomonas leptonychotis strain CCM 8849]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKSGVKTVAV
AVAGSAVITKTIEMEAGLSDDELENQLKMEADQYIPYPLEEVAIDFEVQGVSARNADRVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLIDQLGGGRDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVARSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FAEMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1095990 ACMYQ0_RS15350 WP_136663373.1 3314098..3315162(-) (pilM) [Pseudomonas leptonychotis strain CCM 8849]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATACGCTACTCGGGATCGATATCAGTTCGACCTCCGTCAAGCTCCTCGAATT
GAGCCGCTCCGGAAGCCGCTACAAAGTAGAGGCTTACGCAGTCGAGCCGCTCCCACCAAACGCGGTGGTCGAAAAGAACA
TCGCCGAGTTGGAGGGGGTGGGGCAGGCCCTTTCACGCGTGCTCGCCAAAGCCAAAAGCGGTGTGAAAACCGTTGCGGTG
GCGGTGGCAGGTTCGGCGGTGATTACCAAGACCATCGAGATGGAGGCTGGGCTTTCTGACGATGAGCTGGAAAATCAGCT
GAAGATGGAGGCCGACCAGTACATCCCTTATCCGCTGGAAGAAGTCGCTATCGACTTTGAAGTTCAGGGTGTGTCTGCGC
GCAATGCAGATCGTGTCGAAGTGCTGCTTGCCGCTTGCCGCAAAGAAAACGTTGAGGTCCGTGAGGCCGCGTTAGCCCTC
GCGGGTCTTACCGCCAAAGTGGTTGATGTTGAGGCGTACGCACTCGAGCGTGCATATGGCTTGCTGATCGATCAGCTCGG
CGGCGGTCGCGATGAGTTGACCGTTGCGGTTGTTGATATTGGCGCAACTATGACCACCCTCAGTGTTCTGCACAATGGCC
GCACCATTTACACGCGAGAACAGCTATTCGGCGGCAAACAGCTGACCGAGGAAATCCAGCGACGTTATGGTTTGTCCGTT
GAAGAGGCGGGTCTTGCCAAGAAGCAAGGCGGATTGCCGGATGATTATGACAGTGAAGTACTGCAGCCATTTAAAGAGGC
TGTGGTTCAGCAGGTTGCTCGTTCTCTGCAGTTCTTCTTCGCCGCAGGCCAGTTCAATGATGTTGACTACATCTTGTTGG
CAGGTGGTACCGCGTCAATTCCCGATCTCGATCGTTTGATCCAGCAAAAAATAGGCACCCAGACGCTGGTCGCCAATCCA
TTCGCTGAGATGGCGCTGAGTAGCAAGGTCAACGCCGGCGCGCTCGCCAGTGATGCGCCTGCCTTGATGATTGCTTGTGG
TCTGGCGATGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4T2A0L6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

46.045

100

0.46

Multiple sequence alignment