Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMGRF_RS14060 Genome accession   NZ_CP179923
Coordinates   3047243..3048973 (-) Length   576 a.a.
NCBI ID   WP_414485325.1    Uniprot ID   -
Organism   Stenotrophomonas sp. EMP41     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3042243..3053973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGRF_RS14045 (ACMGRF_14045) sucC 3042535..3043704 (-) 1170 WP_049416943.1 ADP-forming succinate--CoA ligase subunit beta -
  ACMGRF_RS14050 (ACMGRF_14050) - 3044111..3045724 (+) 1614 WP_049416945.1 sensor histidine kinase -
  ACMGRF_RS14055 (ACMGRF_14055) pilR 3045785..3047158 (+) 1374 WP_049416947.1 sigma-54-dependent transcriptional regulator Regulator
  ACMGRF_RS14060 (ACMGRF_14060) pilB 3047243..3048973 (-) 1731 WP_414485325.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMGRF_RS14065 (ACMGRF_14065) - 3049337..3050209 (+) 873 WP_414485326.1 glycosyltransferase family 2 protein -
  ACMGRF_RS14070 (ACMGRF_14070) - 3050221..3051435 (+) 1215 WP_414485327.1 glycosyltransferase -
  ACMGRF_RS14075 (ACMGRF_14075) - 3051479..3052675 (+) 1197 WP_262224786.1 polysaccharide biosynthesis protein -
  ACMGRF_RS14080 (ACMGRF_14080) - 3052638..3053753 (-) 1116 WP_032955899.1 glycosyltransferase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62210.13 Da        Isoelectric Point: 5.8733

>NTDB_id=1093865 ACMGRF_RS14060 WP_414485325.1 3047243..3048973(-) (pilB) [Stenotrophomonas sp. EMP41]
MNAVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFRRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALSGD
DDGIGNLDVSGGDEDISGAGDAGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPANLPEH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKA
AAGITSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=1093865 ACMGRF_RS14060 WP_414485325.1 3047243..3048973(-) (pilB) [Stenotrophomonas sp. EMP41]
ATGAACGCCGTAACCACCGCCAACCTCGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTCCAGGATGGTGCGCTGGACGA
GGCTTCCGCCCGCGACGCCATGGCCAAGGCCGCTGCGGCCCGCCAGCCGCTGCCGACCTGGTTCGCGCAGAACAAGGTGG
TCGGGGCGGCCCAGTTGGCGGCGGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAGGCGCGGCGGCAAGCT
GTTCGTGGGCACCAGCAACCCGACCCATTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTGGAGCCGATCCTGG
TGGATGAGGACCAGATCCGCCGCACGCTCGAGCAGTGGCATGCCAGCCATGACACGCTGGGCGACGCGCTGAGCGGTGAC
GACGATGGCATTGGCAACCTGGATGTGTCCGGCGGTGACGAGGACATTTCCGGAGCCGGCGACGCCGGCATCGACGCCAA
GGGCGATGACACCCCGGTGGTGAAATTCGTCAACAAGGTGCTGGTGGACGCGATCCGCAAGGGCGCCTCGGATATCCACT
TCGAGCCCTATGAAGATGACTACCGCGTGCGCCTGCGCATCGATGGCCTGCTGAAGATGGTCGCGCGCGCGCCGGTGAAG
CTGAACCAGCGCATCGCCGCACGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGACG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAACCGGACCAGCAGAAGCTGTTC
CTGGAGGCCATCCACAAGCCGTACGGCATGGTGCTGGTAACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTGA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCTGCGGCGCTGCGCTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTC
CACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCT
CGGTCACGCTGGTGATCGCGCAGCGCCTGGCGCGCCGGCTGTGTGGCAACTGCAAGCGACCGGCCAACCTGCCCGAGCAT
GCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGACGCGGGTATCGAGCTGTACGAGGCGGTCGGCTGTGATGAGTG
CACCGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGCCACGATCGTGCTGGCTG
GCGGCAATGCGTTGCAGATTGCCGAGGCCGCGCAGCAGATCGGGGTGAACGATCTGCGCCAGTCGGCGTTGAAGAAGGCG
GCGGCGGGCATTACCAGCCTGGCCGAGATCAACCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.44

100

0.557
  pilB Acinetobacter baylyi ADP1

54.623

100

0.554
  pilB Legionella pneumophila strain ERS1305867

51.923

99.306

0.516
  pilF Neisseria gonorrhoeae MS11

49.296

98.611

0.486
  pilB Vibrio cholerae strain A1552

48.252

99.306

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

45.312

100

0.453
  pilB Vibrio campbellii strain DS40M4

45.184

99.132

0.448

Multiple sequence alignment