Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   ACMGE3_RS00250 Genome accession   NZ_CP179736
Coordinates   47883..48401 (-) Length   172 a.a.
NCBI ID   WP_414045400.1    Uniprot ID   -
Organism   Macrococcus sp. 18KM583     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 45747..86667 47883..48401 within 0
IS/Tn 46587..47546 47883..48401 flank 337


Gene organization within MGE regions


Location: 45747..86667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGE3_RS00235 (ACMGE3_00235) - 45747..46295 (-) 549 WP_414055528.1 YfbU family protein -
  ACMGE3_RS00240 (ACMGE3_00240) - 46587..47546 (+) 960 WP_414055529.1 IS30 family transposase -
  ACMGE3_RS00245 (ACMGE3_00245) rpsR 47607..47849 (-) 243 WP_096077771.1 30S ribosomal protein S18 -
  ACMGE3_RS00250 (ACMGE3_00250) ssbA 47883..48401 (-) 519 WP_414045400.1 single-stranded DNA-binding protein Machinery gene
  ACMGE3_RS00255 (ACMGE3_00255) rpsF 48426..48716 (-) 291 WP_096077769.1 30S ribosomal protein S6 -
  ACMGE3_RS00260 (ACMGE3_00260) - 48840..49163 (-) 324 WP_414047387.1 hypothetical protein -
  ACMGE3_RS00265 (ACMGE3_00265) ychF 49182..50282 (-) 1101 WP_414045398.1 redox-regulated ATPase YchF -
  ACMGE3_RS00270 (ACMGE3_00270) - 50295..50486 (-) 192 WP_414045397.1 DUF951 domain-containing protein -
  ACMGE3_RS00275 (ACMGE3_00275) - 50476..51363 (-) 888 WP_414045396.1 mechanosensitive ion channel family protein -
  ACMGE3_RS00280 (ACMGE3_00280) - 51373..52221 (-) 849 WP_414047389.1 ParB/RepB/Spo0J family partition protein -
  ACMGE3_RS00285 (ACMGE3_00285) - 52214..52975 (-) 762 WP_414045394.1 ParA family protein -
  ACMGE3_RS00290 (ACMGE3_00290) noc 53088..53933 (-) 846 WP_414055530.1 nucleoid occlusion protein -
  ACMGE3_RS00295 (ACMGE3_00295) rsmG 53982..54698 (-) 717 WP_414055531.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  ACMGE3_RS00300 (ACMGE3_00300) mnmG 54699..56573 (-) 1875 WP_414055532.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG -
  ACMGE3_RS00305 (ACMGE3_00305) mnmE 56586..57968 (-) 1383 WP_414047393.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  ACMGE3_RS00310 (ACMGE3_00310) jag 58100..58882 (-) 783 WP_414055533.1 RNA-binding cell elongation regulator Jag/EloR -
  ACMGE3_RS00315 (ACMGE3_00315) rnpA 58904..59251 (-) 348 WP_414045388.1 ribonuclease P protein component -
  ACMGE3_RS00320 (ACMGE3_00320) rpmH 59355..59492 (-) 138 WP_041636245.1 50S ribosomal protein L34 -
  ACMGE3_RS00325 (ACMGE3_00325) dnaA 59964..61295 (+) 1332 WP_414045387.1 chromosomal replication initiator protein DnaA -
  ACMGE3_RS00330 (ACMGE3_00330) dnaN 61471..62604 (+) 1134 WP_414055534.1 DNA polymerase III subunit beta -
  ACMGE3_RS00335 (ACMGE3_00335) - 62716..63612 (+) 897 WP_414045385.1 ABC transporter ATP-binding protein -
  ACMGE3_RS00340 (ACMGE3_00340) - 63605..64846 (+) 1242 WP_414055535.1 ABC transporter permease -
  ACMGE3_RS00345 (ACMGE3_00345) - 64965..65681 (+) 717 WP_414045383.1 DsbA family protein -
  ACMGE3_RS00350 (ACMGE3_00350) - 65681..66100 (+) 420 WP_414045382.1 disulfide oxidoreductase -
  ACMGE3_RS00355 (ACMGE3_00355) yaaA 66248..66466 (+) 219 WP_414045381.1 S4 domain-containing protein YaaA -
  ACMGE3_RS00360 (ACMGE3_00360) recF 66466..67575 (+) 1110 WP_414045380.1 DNA replication/repair protein RecF -
  ACMGE3_RS00365 (ACMGE3_00365) gyrB 67592..69505 (+) 1914 WP_414045379.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  ACMGE3_RS00370 (ACMGE3_00370) gyrA 69533..72196 (+) 2664 WP_414055536.1 DNA gyrase subunit A -
  ACMGE3_RS00375 (ACMGE3_00375) alsS 72361..74034 (+) 1674 WP_414055537.1 acetolactate synthase AlsS -
  ACMGE3_RS00380 (ACMGE3_00380) budA 74043..74744 (+) 702 WP_414054619.1 acetolactate decarboxylase -
  ACMGE3_RS00385 (ACMGE3_00385) - 74799..75611 (-) 813 WP_414045375.1 NAD(P)H-hydrate dehydratase -
  ACMGE3_RS00390 (ACMGE3_00390) serS 76002..77276 (+) 1275 WP_414045374.1 serine--tRNA ligase -
  ACMGE3_RS00395 (ACMGE3_00395) - 77523..78038 (+) 516 WP_414056130.1 YceI family protein -
  ACMGE3_RS00400 (ACMGE3_00400) - 78173..79093 (+) 921 WP_414045373.1 DUF2232 domain-containing protein -
  ACMGE3_RS00405 (ACMGE3_00405) - 79118..81088 (+) 1971 WP_414045372.1 DHH family phosphoesterase -
  ACMGE3_RS00410 (ACMGE3_00410) rplI 81088..81534 (+) 447 WP_414045371.1 50S ribosomal protein L9 -
  ACMGE3_RS00415 (ACMGE3_00415) dnaB 81553..82950 (+) 1398 WP_414045370.1 replicative DNA helicase -
  ACMGE3_RS00420 (ACMGE3_00420) - 83261..84556 (+) 1296 WP_414045369.1 adenylosuccinate synthase -
  ACMGE3_RS00425 (ACMGE3_00425) - 84617..85504 (-) 888 WP_414045368.1 YitT family protein -
  ACMGE3_RS00440 (ACMGE3_00440) yycF 85966..86667 (+) 702 WP_414045367.1 response regulator YycF -

Sequence

Protein


Download         Length: 172 a.a.        Molecular weight: 18999.60 Da        Isoelectric Point: 4.6856

>NTDB_id=1093141 ACMGE3_RS00250 WP_414045400.1 47883..48401(-) (ssbA) [Macrococcus sp. 18KM583]
MLNRVVLVGRLTKDPEYRVTPSGVSVATFTLAVNRTFTNAQGERQADFINCVVFRKQAENVNNFLSKGSLAGVDGRLQSR
SYDNQEGRRVYVTEVVCDSVQFLEPKNANQGRSNNDPGQDYSSYDQGNYGQQTQGQNYQSNNQQQSNTSAPSTNPFANAT
GPIDISDDDLPF

Nucleotide


Download         Length: 519 bp        

>NTDB_id=1093141 ACMGE3_RS00250 WP_414045400.1 47883..48401(-) (ssbA) [Macrococcus sp. 18KM583]
TTGCTTAACCGAGTTGTATTAGTAGGTCGTTTAACTAAAGATCCAGAATACAGAGTCACACCATCAGGCGTGTCAGTTGC
TACATTTACATTAGCAGTTAATCGCACATTCACAAATGCGCAAGGTGAAAGACAAGCTGATTTTATCAACTGTGTTGTTT
TCCGTAAGCAAGCTGAGAATGTGAATAACTTCTTAAGCAAGGGTAGCTTAGCTGGGGTAGATGGCAGATTGCAATCACGT
AGTTATGATAATCAGGAAGGCCGTAGAGTATATGTAACGGAAGTCGTTTGTGATTCAGTACAGTTCTTAGAACCGAAAAA
CGCAAATCAAGGCCGTTCTAATAATGATCCAGGTCAGGACTATTCTAGTTATGATCAAGGAAATTATGGACAACAAACTC
AAGGTCAGAATTATCAATCGAATAATCAACAACAATCAAATACATCAGCACCTTCAACGAATCCATTTGCAAATGCAACT
GGACCAATTGATATTAGTGATGATGATTTACCGTTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

60.556

100

0.634
  ssb Latilactobacillus sakei subsp. sakei 23K

54.802

100

0.564
  ssb Glaesserella parasuis strain SC1401

34.783

100

0.372
  ssbB Bacillus subtilis subsp. subtilis str. 168

59.434

61.628

0.366
  ssb Vibrio cholerae strain A1552

34.444

100

0.36

Multiple sequence alignment