Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   ACMGE5_RS03180 Genome accession   NZ_CP179734
Coordinates   579899..580417 (+) Length   172 a.a.
NCBI ID   WP_414045400.1    Uniprot ID   -
Organism   Macrococcus sp. 18KM444     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 573602..599645 579899..580417 within 0


Gene organization within MGE regions


Location: 573602..599645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGE5_RS03135 (ACMGE5_03135) rsmG 573602..574318 (+) 717 WP_414045392.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  ACMGE5_RS03140 (ACMGE5_03140) noc 574367..575212 (+) 846 WP_414045393.1 nucleoid occlusion protein -
  ACMGE5_RS03145 (ACMGE5_03145) - 575325..576086 (+) 762 WP_414045394.1 ParA family protein -
  ACMGE5_RS03150 (ACMGE5_03150) - 576079..576927 (+) 849 WP_414045395.1 ParB/RepB/Spo0J family partition protein -
  ACMGE5_RS03155 (ACMGE5_03155) - 576937..577824 (+) 888 WP_414045396.1 mechanosensitive ion channel family protein -
  ACMGE5_RS03160 (ACMGE5_03160) - 577814..578005 (+) 192 WP_414045397.1 DUF951 domain-containing protein -
  ACMGE5_RS03165 (ACMGE5_03165) ychF 578018..579118 (+) 1101 WP_414045398.1 redox-regulated ATPase YchF -
  ACMGE5_RS03170 (ACMGE5_03170) - 579137..579460 (+) 324 WP_414045399.1 hypothetical protein -
  ACMGE5_RS03175 (ACMGE5_03175) rpsF 579584..579874 (+) 291 WP_096077769.1 30S ribosomal protein S6 -
  ACMGE5_RS03180 (ACMGE5_03180) ssbA 579899..580417 (+) 519 WP_414045400.1 single-stranded DNA-binding protein Machinery gene
  ACMGE5_RS03185 (ACMGE5_03185) rpsR 580451..580693 (+) 243 WP_096077771.1 30S ribosomal protein S18 -
  ACMGE5_RS03190 (ACMGE5_03190) - 580936..581484 (+) 549 WP_414045401.1 YfbU family protein -
  ACMGE5_RS03195 (ACMGE5_03195) - 581540..582148 (-) 609 WP_414045402.1 GNAT family N-acetyltransferase -
  ACMGE5_RS03200 (ACMGE5_03200) - 582231..582449 (-) 219 WP_414045403.1 DUF2382 domain-containing protein -
  ACMGE5_RS03205 (ACMGE5_03205) - 582567..583568 (-) 1002 WP_414045404.1 DUF2382 domain-containing protein -
  ACMGE5_RS03210 (ACMGE5_03210) guaB 584206..585678 (+) 1473 WP_414045405.1 IMP dehydrogenase -
  ACMGE5_RS03215 (ACMGE5_03215) guaA 585705..587246 (+) 1542 WP_414045406.1 glutamine-hydrolyzing GMP synthase -
  ACMGE5_RS03220 (ACMGE5_03220) - 587384..587839 (-) 456 WP_414045407.1 type II toxin-antitoxin system death-on-curing family toxin -
  ACMGE5_RS03225 (ACMGE5_03225) - 587836..588849 (-) 1014 WP_414045408.1 hypothetical protein -
  ACMGE5_RS03230 (ACMGE5_03230) - 588860..590095 (-) 1236 WP_414045409.1 tyrosine-type recombinase/integrase -
  ACMGE5_RS03235 (ACMGE5_03235) - 590099..590710 (-) 612 WP_130921233.1 helix-turn-helix domain-containing protein -
  ACMGE5_RS03240 (ACMGE5_03240) - 590836..591042 (+) 207 WP_414045410.1 helix-turn-helix transcriptional regulator -
  ACMGE5_RS03245 (ACMGE5_03245) - 591078..591302 (+) 225 WP_414045411.1 helix-turn-helix transcriptional regulator -
  ACMGE5_RS03250 (ACMGE5_03250) - 591302..591577 (+) 276 WP_414045412.1 helix-turn-helix domain-containing protein -
  ACMGE5_RS03255 (ACMGE5_03255) - 591592..591762 (+) 171 WP_414045413.1 hypothetical protein -
  ACMGE5_RS03260 (ACMGE5_03260) - 591765..592247 (+) 483 WP_414045414.1 hypothetical protein -
  ACMGE5_RS03265 (ACMGE5_03265) - 592250..592552 (+) 303 WP_414045415.1 hypothetical protein -
  ACMGE5_RS03270 (ACMGE5_03270) - 592618..593538 (+) 921 WP_414045416.1 DnaD domain protein -
  ACMGE5_RS03275 (ACMGE5_03275) - 593541..594347 (+) 807 WP_414045417.1 ATP-binding protein -
  ACMGE5_RS03280 (ACMGE5_03280) - 594473..594727 (+) 255 WP_414045418.1 hypothetical protein -
  ACMGE5_RS03285 (ACMGE5_03285) - 594990..595568 (+) 579 WP_414045419.1 hypothetical protein -
  ACMGE5_RS03290 (ACMGE5_03290) - 595568..595828 (+) 261 WP_130921225.1 hypothetical protein -
  ACMGE5_RS03295 (ACMGE5_03295) - 595893..596315 (+) 423 WP_414045420.1 terminase small subunit -
  ACMGE5_RS03300 (ACMGE5_03300) - 596349..596837 (+) 489 WP_414045421.1 terminase small subunit -
  ACMGE5_RS03305 (ACMGE5_03305) - 596862..597365 (+) 504 WP_414046910.1 spore coat protein -
  ACMGE5_RS03310 (ACMGE5_03310) - 597362..597733 (+) 372 WP_414045423.1 hypothetical protein -
  ACMGE5_RS03315 (ACMGE5_03315) - 597950..598681 (+) 732 WP_414045424.1 hypothetical protein -
  ACMGE5_RS03320 (ACMGE5_03320) - 599277..599645 (+) 369 WP_414045425.1 nuclear transport factor 2 family protein -

Sequence


Protein


Download         Length: 172 a.a.        Molecular weight: 18999.60 Da        Isoelectric Point: 4.6856

>NTDB_id=1093081 ACMGE5_RS03180 WP_414045400.1 579899..580417(+) (ssbA) [Macrococcus sp. 18KM444]
MLNRVVLVGRLTKDPEYRVTPSGVSVATFTLAVNRTFTNAQGERQADFINCVVFRKQAENVNNFLSKGSLAGVDGRLQSR
SYDNQEGRRVYVTEVVCDSVQFLEPKNANQGRSNNDPGQDYSSYDQGNYGQQTQGQNYQSNNQQQSNTSAPSTNPFANAT
GPIDISDDDLPF

Nucleotide


Download         Length: 519 bp        

>NTDB_id=1093081 ACMGE5_RS03180 WP_414045400.1 579899..580417(+) (ssbA) [Macrococcus sp. 18KM444]
TTGCTTAACCGAGTTGTATTAGTAGGTCGTTTAACTAAAGATCCAGAATACAGAGTCACACCATCAGGCGTGTCAGTTGC
TACATTTACATTAGCAGTTAATCGCACATTCACAAATGCGCAAGGTGAAAGACAAGCTGATTTTATCAACTGTGTTGTTT
TCCGTAAGCAAGCTGAGAATGTGAATAACTTCTTAAGCAAGGGTAGCTTAGCTGGGGTAGATGGCAGATTGCAATCACGT
AGTTATGATAATCAGGAAGGCCGTAGAGTATATGTAACGGAAGTCGTTTGTGATTCAGTACAGTTCTTAGAACCGAAAAA
CGCAAATCAAGGCCGTTCTAATAATGATCCAGGTCAGGACTATTCTAGTTATGATCAAGGAAATTATGGACAACAAACTC
AAGGTCAGAATTATCAATCGAATAATCAACAACAATCAAATACATCAGCACCTTCAACGAATCCATTTGCAAATGCAACT
GGACCAATTGATATTAGTGATGATGATTTACCGTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

60.556

100

0.634

  ssb Latilactobacillus sakei subsp. sakei 23K

54.802

100

0.564

  ssb Glaesserella parasuis strain SC1401

34.783

100

0.372

  ssbB Bacillus subtilis subsp. subtilis str. 168

59.434

61.628

0.366

  ssb Vibrio cholerae strain A1552

34.444

100

0.36


Multiple sequence alignment