Detailed information NTG1091823

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACLKL2_RS14825
• Genome accession: NZ_CP178827
• Coordinates: 3049737..3050891 (+)
• Length: 384 a.a.
• NCBI ID: WP_113817613.1
• Uniprot ID: -
• Organism: Pseudoalteromonas sp. KJ10-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3044737..3055891

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLKL2_RS14810 (ACLKL2_14810)	-	3045264..3047108 (-)	1845	WP_413692320.1	DUF294 nucleotidyltransferase-like domain-containing protein	-
ACLKL2_RS14815 (ACLKL2_14815)	-	3047194..3048912 (-)	1719	WP_113817615.1	sodium:solute symporter family protein	-
ACLKL2_RS14820 (ACLKL2_14820)	-	3048928..3049191 (-)	264	WP_113817614.1	DUF4212 domain-containing protein	-
ACLKL2_RS14825 (ACLKL2_14825)	pilU	3049737..3050891 (+)	1155	WP_113817613.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACLKL2_RS14855 (ACLKL2_14855)	-	3051918..3053213 (-)	1296	WP_413692321.1	hypothetical protein	-
ACLKL2_RS14860 (ACLKL2_14860)	dnaQ	3053204..3053920 (-)	717	WP_413692322.1	DNA polymerase III subunit epsilon	-
ACLKL2_RS14865 (ACLKL2_14865)	rnhA	3054021..3054473 (+)	453	WP_413694337.1	ribonuclease HI	-
ACLKL2_RS14870 (ACLKL2_14870)	-	3054470..3055204 (-)	735	WP_413692323.1	class I SAM-dependent methyltransferase	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 42657.94 Da
• Isoelectric Point: 6.0217

>NTDB_id=1091823 ACLKL2_RS14825 WP_113817613.1 3049737..3050891(+) (pilU) [Pseudoalteromonas sp. KJ10-2]
MEFNTMLSQLSEQDGSDLYLATGAPPSAKFQGELIPLSEISFKPGDVKAIAYDIMDPEQIEEFEHTLEMNLAISIGGIGR
FRVNIFKQRNDVSIVVRNIKINIPKFADLRLPEILKKVVMAKNGLILFVGATGSGKSTSLAALIDHRNENSSGHIVTIED
PVEFVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDTETMEHALAFSETGHLVLSTLHANNANQAIDRI
INFFTEERRAQLLMDLGNNLRAFVSQRLIPTIDGKRCAAIEVMMGTLTIRDMVKRGHFESLKEVMEKSKELGMQTFDSAL
FDLVKEGLISEQEAIKNSDSPTNLALKFKLDKGNFVEADVQPKAKSSLSLEPKGLGDLNDLTYS

Nucleotide

• Length: 1155 bp

>NTDB_id=1091823 ACLKL2_RS14825 WP_113817613.1 3049737..3050891(+) (pilU) [Pseudoalteromonas sp. KJ10-2]
ATGGAATTTAATACAATGTTGAGTCAATTATCCGAACAAGATGGTTCTGACCTTTATTTAGCAACGGGCGCGCCTCCTTC
AGCTAAATTTCAAGGAGAATTGATTCCACTAAGTGAAATATCTTTTAAGCCAGGTGATGTTAAGGCTATTGCTTATGACA
TTATGGATCCTGAGCAAATAGAAGAGTTTGAACATACTTTAGAGATGAATTTAGCTATTTCTATCGGTGGAATAGGGCGT
TTTCGTGTCAATATATTTAAACAGCGCAATGACGTCTCTATTGTTGTTCGTAATATTAAAATTAATATTCCTAAGTTTGC
TGATTTAAGGTTACCTGAAATACTAAAAAAAGTAGTGATGGCTAAAAATGGTTTGATACTGTTTGTTGGCGCGACGGGGT
CAGGTAAATCGACTTCACTAGCGGCTTTAATTGATCACCGTAATGAAAACTCATCTGGGCATATTGTAACCATTGAAGAT
CCCGTTGAGTTCGTTCATCGACATAAAAAAAGCATTATTAATCAAAGAGAAGTAGGGGTTGATACCCGAAGTTTTCATGC
TGCGCTGAAAAATACACTAAGACAGGCTCCTGATGTTATTTTAATCGGTGAAATACGTGATACTGAAACCATGGAGCATG
CATTAGCTTTTTCTGAAACGGGGCATTTAGTGTTATCTACACTGCATGCAAATAATGCAAACCAAGCGATTGACCGTATT
ATTAACTTTTTTACCGAAGAGCGACGTGCACAGTTATTAATGGATTTAGGTAATAACTTGAGGGCCTTTGTTTCTCAACG
TTTAATTCCCACCATTGATGGTAAACGTTGTGCTGCCATTGAAGTCATGATGGGCACATTAACCATTCGAGACATGGTAA
AACGCGGGCACTTTGAAAGCTTAAAAGAGGTGATGGAAAAGTCTAAAGAGTTAGGAATGCAAACTTTTGACAGTGCTTTA
TTCGATTTAGTGAAGGAAGGTTTAATCAGTGAGCAAGAGGCGATTAAAAACTCTGATTCTCCGACTAACTTAGCACTTAA
ATTTAAATTGGATAAAGGTAATTTTGTTGAAGCTGACGTGCAACCTAAAGCTAAAAGTAGCCTGAGTTTAGAGCCAAAGG
GATTGGGTGACCTTAACGATTTAACTTATTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (58-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.131	91.406	0.495
pilU	Acinetobacter baylyi ADP1	53.541	91.927	0.492
pilU	Vibrio cholerae strain A1552	48.169	92.448	0.445
pilT	Pseudomonas aeruginosa PAK	40.407	89.583	0.362
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.173	90.104	0.362