Detailed information NTG1090937

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACL6EO_RS12605
• Genome accession: NZ_CP178718
• Coordinates: 2589967..2590581 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain SY1154
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2584967..2595581

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACL6EO_RS12575 (ACL6EO_12575)	rpsT	2585270..2585536 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACL6EO_RS12580 (ACL6EO_12580)	holA	2585499..2586594 (-)	1096	Protein_2431	DNA polymerase III subunit delta	-
ACL6EO_RS12585 (ACL6EO_12585)	-	2586634..2586785 (-)	152	Protein_2432	hypothetical protein	-
ACL6EO_RS12590 (ACL6EO_12590)	-	2586826..2586960 (+)	135	WP_003152870.1	YqzM family protein	-
ACL6EO_RS12595 (ACL6EO_12595)	comEC	2586979..2589330 (-)	2352	WP_276350933.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACL6EO_RS12600 (ACL6EO_12600)	-	2589331..2589900 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACL6EO_RS12605 (ACL6EO_12605)	comEA	2589967..2590581 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACL6EO_RS12610 (ACL6EO_12610)	comER	2590640..2591461 (+)	822	WP_029973401.1	late competence protein ComER	-
ACL6EO_RS12615 (ACL6EO_12615)	-	2591530..2592267 (-)	738	WP_014418421.1	class I SAM-dependent DNA methyltransferase	-
ACL6EO_RS12620 (ACL6EO_12620)	rsfS	2592264..2592620 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACL6EO_RS12625 (ACL6EO_12625)	yqeK	2592638..2593198 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACL6EO_RS12630 (ACL6EO_12630)	-	2593188..2593757 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACL6EO_RS12635 (ACL6EO_12635)	yhbY	2593768..2594058 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACL6EO_RS12640 (ACL6EO_12640)	aroE	2594052..2594894 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=1090937 ACL6EO_RS12605 WP_014418419.1 2589967..2590581(-) (comEA) [Bacillus velezensis strain SY1154]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1090937 ACL6EO_RS12605 WP_014418419.1 2589967..2590581(-) (comEA) [Bacillus velezensis strain SY1154]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363