Detailed information NTG1090489

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACKUFW_RS12260
• Genome accession: NZ_CP178610
• Coordinates: 2523419..2524033 (-)
• Length: 204 a.a.
• NCBI ID: WP_166706122.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain GUMHT p116
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2518419..2529033

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACKUFW_RS12230 (ACKUFW_12230)	rpsT	2518719..2518985 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACKUFW_RS12235 (ACKUFW_12235)	holA	2519002..2520043 (-)	1042	Protein_2362	DNA polymerase III subunit delta	-
ACKUFW_RS12240 (ACKUFW_12240)	-	2520083..2520237 (-)	155	Protein_2363	hypothetical protein	-
ACKUFW_RS12245 (ACKUFW_12245)	-	2520278..2520412 (+)	135	WP_003152870.1	YqzM family protein	-
ACKUFW_RS12250 (ACKUFW_12250)	comEC	2520431..2522782 (-)	2352	WP_202735636.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACKUFW_RS12255 (ACKUFW_12255)	-	2522783..2523352 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACKUFW_RS12260 (ACKUFW_12260)	comEA	2523419..2524033 (-)	615	WP_166706122.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACKUFW_RS12265 (ACKUFW_12265)	comER	2524092..2524913 (+)	822	WP_015240246.1	late competence protein ComER	-
ACKUFW_RS12270 (ACKUFW_12270)	-	2524982..2525719 (-)	738	WP_166706119.1	class I SAM-dependent DNA methyltransferase	-
ACKUFW_RS12275 (ACKUFW_12275)	rsfS	2525716..2526072 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACKUFW_RS12280 (ACKUFW_12280)	yqeK	2526090..2526650 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACKUFW_RS12285 (ACKUFW_12285)	-	2526640..2527209 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
ACKUFW_RS12290 (ACKUFW_12290)	yhbY	2527220..2527510 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACKUFW_RS12295 (ACKUFW_12295)	aroE	2527504..2528346 (-)	843	WP_015417858.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22220.30 Da
• Isoelectric Point: 5.1985

>NTDB_id=1090489 ACKUFW_RS12260 WP_166706122.1 2523419..2524033(-) (comEA) [Bacillus velezensis strain GUMHT p116]
MTKAMMTERLRWNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1090489 ACKUFW_RS12260 WP_166706122.1 2523419..2524033(-) (comEA) [Bacillus velezensis strain GUMHT p116]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGTGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Lactococcus lactis subsp. cremoris KW2	33.778	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.058	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.577	100	0.363
comEA	Staphylococcus aureus MW2	34.579	100	0.363