Detailed information NTG1089908

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACKUFS_RS24290
• Genome accession: NZ_CP178532
• Coordinates: 5387490..5388554 (+)
• Length: 354 a.a.
• NCBI ID: WP_007248061.1
• Uniprot ID: A0A3M3ZTK3
• Organism: Pseudomonas cannabina strain YM7930
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5382490..5393554

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACKUFS_RS24280 (ACKUFS_24280)	-	5383381..5384649 (+)	1269	WP_007248063.1	malic enzyme-like NAD(P)-binding protein	-
ACKUFS_RS24285 (ACKUFS_24285)	-	5384836..5387280 (-)	2445	WP_371745306.1	penicillin-binding protein 1A	-
ACKUFS_RS24290 (ACKUFS_24290)	pilM	5387490..5388554 (+)	1065	WP_007248061.1	pilus assembly protein PilM	Machinery gene
ACKUFS_RS24295 (ACKUFS_24295)	-	5388554..5389129 (+)	576	WP_007248060.1	PilN domain-containing protein	-
ACKUFS_RS24300 (ACKUFS_24300)	pilO	5389126..5389749 (+)	624	WP_007248059.1	type 4a pilus biogenesis protein PilO	-
ACKUFS_RS24305 (ACKUFS_24305)	pilP	5389746..5390273 (+)	528	WP_007248058.1	type 4a pilus biogenesis lipoprotein PilP	-
ACKUFS_RS24310 (ACKUFS_24310)	pilQ	5390321..5392399 (+)	2079	WP_007248057.1	type IV pilus secretin PilQ	Machinery gene
ACKUFS_RS24315 (ACKUFS_24315)	aroK	5392404..5392922 (+)	519	WP_007248056.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38106.62 Da
• Isoelectric Point: 4.7008

>NTDB_id=1089908 ACKUFS_RS24290 WP_007248061.1 5387490..5388554(+) (pilM) [Pseudomonas cannabina strain YM7930]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1089908 ACKUFS_RS24290 WP_007248061.1 5387490..5388554(+) (pilM) [Pseudomonas cannabina strain YM7930]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCCTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCTGAACTCGAAGGGGTTGGCCAGGCGTTGTCTCGCGTGCTGGTCAAGGCCAAGACCAGCGTCAAGATTGTGGCGGTT
GCAGTGGCCGGGTCCGCAGTCATCACCAAAACCATCGAGATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATTCCCTATCCGCTTGAAGAAGTGGCCATCGATTTTGAAGTGCAGGGCTATTCGGTAC
GCAATCCGGAACGGGTTGAAGTGCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCGGCGCTGGCGCTG
GCCGGGCTGACCGCGCGCGTTGTTGACGTCGAAGCCTATGCGTTGGAGCGCTCGTTCGGCCTGCTGGCTGCGCAATTGGG
GAACGGCCACGACGAGCTGACCGTTGCGGTGGTCGATATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACCATGGGC
GGATCATCTATACCCGCGAGCAACTGTTCGGCGGCCGCCAGCTGACCGACGAGATTCAGCGCCGCTATGGCCTGTCCATG
GAAGAGGCCGGGCTTGCCAAGAAACAGGGCGGGCTGCCTGACGACTATGTCAGCGAGGTGCTGGACCCGTTCAAGGACGC
GCTGGTGCAGCAGGTTTCCCGCTCGTTGCAGTTCTTCTTTGCGGCGGGGCAGTACAACTCGGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCGATTTCCGGGCTGGAGCACCTGATTCAGCGACGTATCGGCACGCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGTTCCAAGGTCAATGCGGGCGCACTGGCCAGTGACGCCCCGGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A3M3ZTK3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469