Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACKUFS_RS24310 Genome accession   NZ_CP178532
Coordinates   5390321..5392399 (+) Length   692 a.a.
NCBI ID   WP_007248057.1    Uniprot ID   -
Organism   Pseudomonas cannabina strain YM7930     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5385321..5397399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUFS_RS24290 (ACKUFS_24290) pilM 5387490..5388554 (+) 1065 WP_007248061.1 pilus assembly protein PilM Machinery gene
  ACKUFS_RS24295 (ACKUFS_24295) - 5388554..5389129 (+) 576 WP_007248060.1 PilN domain-containing protein -
  ACKUFS_RS24300 (ACKUFS_24300) pilO 5389126..5389749 (+) 624 WP_007248059.1 type 4a pilus biogenesis protein PilO -
  ACKUFS_RS24305 (ACKUFS_24305) pilP 5389746..5390273 (+) 528 WP_007248058.1 type 4a pilus biogenesis lipoprotein PilP -
  ACKUFS_RS24310 (ACKUFS_24310) pilQ 5390321..5392399 (+) 2079 WP_007248057.1 type IV pilus secretin PilQ Machinery gene
  ACKUFS_RS24315 (ACKUFS_24315) aroK 5392404..5392922 (+) 519 WP_007248056.1 shikimate kinase AroK -
  ACKUFS_RS24320 (ACKUFS_24320) aroB 5393039..5394142 (+) 1104 WP_007248055.1 3-dehydroquinate synthase -
  ACKUFS_RS24325 (ACKUFS_24325) - 5394153..5395784 (+) 1632 WP_007248054.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74245.50 Da        Isoelectric Point: 5.1738

>NTDB_id=1089909 ACKUFS_RS24310 WP_007248057.1 5390321..5392399(+) (pilQ) [Pseudomonas cannabina strain YM7930]
MTRILSIIGASFCIAMLSPVVQAANLKTLDVAALPGDRVELKLSFDGPVPAPRGYTTAQPARIALDLPGVTSQLATKSRE
LGTGNARSVVVVQAQDRTRVIIYLTTLSPYSSRVDGNNLFVVIGQGAAAAQASQPSTAMGAARVAVPPPTPASARPFVPA
GAKAIRNIDFQRGELGEGNVVIDLSNANIAPDIQEQGGKIRVDFAKTMLPEPLRVRLDVKDFATPVQFVSASAAGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTHEDMDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKELGVRWGGATNTSGSS
NWSTGTAAGGSTANSSTFVDMGAANATSGIGLGFLTNNTMLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEVPY
QESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYVNAVLGVPPIKKNEVNAKVLISDGETIVIGGVFSN
TQSKSVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=1089909 ACKUFS_RS24310 WP_007248057.1 5390321..5392399(+) (pilQ) [Pseudomonas cannabina strain YM7930]
ATGACCCGGATTCTCTCGATTATCGGCGCGTCGTTTTGCATCGCGATGCTGTCGCCGGTTGTCCAGGCTGCCAACCTAAA
GACCCTGGATGTCGCCGCGTTGCCGGGTGACCGGGTCGAACTCAAGCTGTCTTTCGACGGCCCGGTGCCTGCTCCGCGCG
GTTACACCACAGCGCAACCGGCGCGCATTGCACTCGACCTGCCCGGTGTCACCAGCCAGCTGGCGACCAAAAGTCGTGAG
CTGGGCACCGGTAACGCGCGCAGCGTGGTGGTGGTGCAGGCTCAGGATCGCACGCGTGTGATCATCTACCTGACGACACT
GTCGCCTTACAGCTCGCGGGTCGACGGCAACAACCTGTTCGTGGTCATCGGTCAGGGCGCAGCCGCGGCCCAGGCGTCCC
AGCCGAGTACCGCGATGGGCGCAGCCAGAGTGGCCGTGCCGCCGCCCACCCCGGCGTCCGCCAGGCCTTTTGTGCCGGCC
GGTGCCAAAGCCATCAGGAATATCGATTTCCAGCGCGGTGAGCTGGGTGAAGGCAACGTGGTCATCGACCTGAGCAATGC
CAACATTGCGCCTGACATTCAGGAGCAGGGCGGCAAGATCCGTGTCGACTTCGCCAAAACGATGCTGCCGGAACCCTTGC
GCGTGCGCCTCGACGTCAAGGATTTCGCGACCCCGGTGCAGTTCGTCAGCGCGTCGGCCGCAGGTGACAAGGCAAGCATT
CTGATCGAGCCGTCGGGCGCCTTCGACTATTCGGCGTTCCAGACCGAAAACAAGCTGACCATCAGTGTGCGCCCGCTGAC
TCACGAGGATATGGACCGGCGCGCCGCCGACAAGCCGGTGTACACCGGTGAAAAGCTCTCGCTGAATTTCCAGGACATCG
ATGTGCGCTCGGTGCTGCAGCTGATTGCCGACTTCACCAACCTCAATCTGGTCGCCAGTGACACGGTGCAGGGCGGTATC
ACCCTGCGTCTGCAGAATGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGGCTGGACAAACGCAAGGT
TGGCAGCGTGCTGCTGGTCGCCCCGGCCGATGAAATTGCTGCCCGCGAACGTCAGGAACTGGAGTCGCTCAAGCAGATCG
CCGAGCTGGCGCCGTTGCGTCGTGAGCTGTTGCAGGTCAACTACGCCAAAGCTGCCGATATCGCCAAGCTGTTCCAGTCG
GTGACCAGTGCCGAGTCCAAGGCCGATGAGCGCGGTTCGATCACGGTCGATGACCGGACCAACAACATCATCGCTTACCA
GACCCAGGACCGGCTCGACGAGTTGCGCCGTATTGTTTCGCAGCTGGACATTCCGGTGCGTCAGGTGATGATCGAAGCGC
GCATCGTCGAGGCCAACGTCGATTACGACAAAGAGCTGGGTGTGCGCTGGGGTGGCGCGACCAACACCAGCGGCAGCAGC
AACTGGTCGACGGGCACTGCAGCGGGCGGCTCCACGGCCAACAGCTCCACGTTCGTCGATATGGGCGCTGCCAATGCCAC
CTCCGGCATCGGCCTTGGCTTCCTGACCAACAACACGATGCTCGACCTTGAGCTGACGGCGATGGAAAAGACCGGCAACG
GTGAAATCGTCTCGCAACCCAAGGTGGTCACGTCCGACAAGGAAACCGCCAAGATCCTCAAGGGCACCGAGGTTCCGTAT
CAGGAATCCAGCTCCAGTGGTGCAACCACGGTCAGCTTCAAGGAAGCGTCCCTGTCGCTCGAAGTCACACCGCAGATCAC
CCCCGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACGTGAATGCGGTGCTGGGTGTGCCGC
CGATCAAGAAAAACGAAGTCAACGCCAAAGTGCTGATTTCCGACGGTGAAACCATCGTCATCGGTGGTGTGTTCTCGAAT
ACGCAAAGTAAAAGTGTGGATAAAGTGCCATTTCTTGGCGATGTACCGTATCTTGGCCGGCTTTTCAGGCGCGATGTGGT
ATCCGAGTCCAAATCCGAGCTGTTGGTATTTCTGACTCCGCGTATCATGAACAACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.061

100

0.753
  comQ Acinetobacter baylyi ADP1

41.523

100

0.418
  pilQ Acinetobacter baumannii strain A118

38.587

100

0.41
  pilQ Acinetobacter baumannii D1279779

38.587

100

0.41
  pilQ Legionella pneumophila strain Lp02

37.921

98.699

0.374
  pilQ Legionella pneumophila strain ERS1305867

37.921

98.699

0.374

Multiple sequence alignment