Detailed information NTG1089165

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACLNGX_RS12425
• Genome accession: NZ_CP178374
• Coordinates: 2615028..2615642 (-)
• Length: 204 a.a.
• NCBI ID: WP_166706122.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 2023
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2610028..2620642

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLNGX_RS12395 (ACLNGX_12395)	rpsT	2610328..2610594 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACLNGX_RS12400 (ACLNGX_12400)	holA	2610611..2611652 (-)	1042	Protein_2395	DNA polymerase III subunit delta	-
ACLNGX_RS12405 (ACLNGX_12405)	-	2611692..2611846 (-)	155	Protein_2396	hypothetical protein	-
ACLNGX_RS12410 (ACLNGX_12410)	-	2611887..2612021 (+)	135	WP_003152870.1	YqzM family protein	-
ACLNGX_RS12415 (ACLNGX_12415)	comEC	2612040..2614391 (-)	2352	WP_166706124.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACLNGX_RS12420 (ACLNGX_12420)	-	2614392..2614961 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACLNGX_RS12425 (ACLNGX_12425)	comEA	2615028..2615642 (-)	615	WP_166706122.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACLNGX_RS12430 (ACLNGX_12430)	comER	2615701..2616522 (+)	822	WP_015240246.1	late competence protein ComER	-
ACLNGX_RS12435 (ACLNGX_12435)	-	2616591..2617328 (-)	738	WP_166706119.1	class I SAM-dependent DNA methyltransferase	-
ACLNGX_RS12440 (ACLNGX_12440)	rsfS	2617325..2617681 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACLNGX_RS12445 (ACLNGX_12445)	yqeK	2617699..2618259 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACLNGX_RS12450 (ACLNGX_12450)	-	2618249..2618818 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
ACLNGX_RS12455 (ACLNGX_12455)	yhbY	2618829..2619119 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACLNGX_RS12460 (ACLNGX_12460)	aroE	2619113..2619955 (-)	843	WP_015417858.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22220.30 Da
• Isoelectric Point: 5.1985

>NTDB_id=1089165 ACLNGX_RS12425 WP_166706122.1 2615028..2615642(-) (comEA) [Bacillus velezensis strain 2023]
MTKAMMTERLRWNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1089165 ACLNGX_RS12425 WP_166706122.1 2615028..2615642(-) (comEA) [Bacillus velezensis strain 2023]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGTGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Lactococcus lactis subsp. cremoris KW2	33.778	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.058	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.577	100	0.363
comEA	Staphylococcus aureus MW2	34.579	100	0.363