Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   ACLFAM_RS05295 Genome accession   NZ_CP178226
Coordinates   1046073..1047146 (-) Length   357 a.a.
NCBI ID   WP_080756637.1    Uniprot ID   -
Organism   Lactococcus lactis strain L-WY410     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1041073..1052146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLFAM_RS05255 (ACLFAM_05255) - 1041435..1042244 (-) 810 WP_014570791.1 metal ABC transporter permease -
  ACLFAM_RS05260 (ACLFAM_05260) - 1042237..1042974 (-) 738 WP_012898617.1 metal ABC transporter ATP-binding protein -
  ACLFAM_RS05265 (ACLFAM_05265) - 1043151..1043993 (-) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -
  ACLFAM_RS05270 (ACLFAM_05270) - 1043990..1044427 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  ACLFAM_RS05275 (ACLFAM_05275) comGG 1044509..1044793 (-) 285 WP_025017139.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLFAM_RS05280 (ACLFAM_05280) comGF 1044832..1045314 (-) 483 WP_039116460.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLFAM_RS05285 (ACLFAM_05285) comGD 1045286..1045645 (-) 360 WP_023188582.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLFAM_RS05290 (ACLFAM_05290) comGC 1045677..1045946 (-) 270 WP_023188581.1 competence type IV pilus major pilin ComGC Machinery gene
  ACLFAM_RS05295 (ACLFAM_05295) comGB 1046073..1047146 (-) 1074 WP_080756637.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLFAM_RS05300 (ACLFAM_05300) comGA 1047040..1047977 (-) 938 Protein_1038 competence type IV pilus ATPase ComGA -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40367.57 Da        Isoelectric Point: 10.3001

>NTDB_id=1088558 ACLFAM_RS05295 WP_080756637.1 1046073..1047146(-) (comGB) [Lactococcus lactis strain L-WY410]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQVLSEILESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=1088558 ACLFAM_RS05295 WP_080756637.1 1046073..1047146(-) (comGB) [Lactococcus lactis strain L-WY410]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGTTTTATCAGAGATTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGAACGATGCAATTGGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACATTTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

83.578

95.518

0.798
  comYB Streptococcus gordonii str. Challis substr. CH1

53.959

95.518

0.515
  comYB Streptococcus mutans UA140

53.959

95.518

0.515
  comYB Streptococcus mutans UA159

53.666

95.518

0.513
  comGB/cglB Streptococcus mitis SK321

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae TIGR4

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae Rx1

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae D39

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae R6

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis NCTC 12261

50.448

93.838

0.473

Multiple sequence alignment