Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ABWW24_RS03395 Genome accession   NZ_AP031330
Coordinates   589069..589650 (-) Length   193 a.a.
NCBI ID   WP_143586070.1    Uniprot ID   A0AAD1NYJ2
Organism   Thermus thermophilus strain TF25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 584069..594650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABWW24_RS03375 (TthTF25_06580) - 584126..584680 (-) 555 WP_353300922.1 shikimate kinase -
  ABWW24_RS03380 (TthTF25_06590) aroC 584705..585856 (-) 1152 WP_353300692.1 chorismate synthase -
  ABWW24_RS03385 (TthTF25_06600) pilQ 585915..588188 (-) 2274 WP_353300693.1 secretin N-terminal domain-containing protein Machinery gene
  ABWW24_RS03390 (TthTF25_06610) pilW 588185..589072 (-) 888 WP_353300694.1 competence protein Machinery gene
  ABWW24_RS03395 (TthTF25_06620) pilO 589069..589650 (-) 582 WP_143586070.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABWW24_RS03400 (TthTF25_06630) pilN 589640..590263 (-) 624 WP_011173433.1 competence protein Machinery gene
  ABWW24_RS03405 (TthTF25_06640) pilM 590256..591389 (-) 1134 WP_353300695.1 type IV pilus assembly protein PilM Machinery gene
  ABWW24_RS03410 (TthTF25_06650) - 591526..592530 (-) 1005 WP_244340638.1 homoisocitrate dehydrogenase -
  ABWW24_RS03415 (TthTF25_06660) - 592585..593076 (-) 492 WP_011173430.1 DUF5317 domain-containing protein -
  ABWW24_RS03420 (TthTF25_06670) - 593127..594533 (-) 1407 WP_353300696.1 HD-GYP domain-containing protein -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 21340.64 Da        Isoelectric Point: 6.4566

>NTDB_id=108754 ABWW24_RS03395 WP_143586070.1 589069..589650(-) (pilO) [Thermus thermophilus strain TF25]
MLARLGQREWALLAMVLTALLGLLWYYLLIVPTRQEIATVRQEIDRLTIERNRGLQAQRALPELRATIAALQAQRLAFLR
ALPREERLAEVLSEVLQDAEASGVVVRSFTRSRTSAPVPEVRAVNLALALEAPFPETYAYLRRLEDLSRFSTLSGLNLSV
QSQEANPTLATSLTLTVYVLAQGVEAETGGSTP

Nucleotide


Download         Length: 582 bp        

>NTDB_id=108754 ABWW24_RS03395 WP_143586070.1 589069..589650(-) (pilO) [Thermus thermophilus strain TF25]
GTGCTCGCTAGGCTAGGACAGCGGGAGTGGGCCCTCCTGGCCATGGTCCTCACCGCCCTCCTGGGGCTCCTCTGGTACTA
CCTGCTCATCGTGCCCACGAGGCAGGAGATCGCCACCGTCCGGCAGGAGATTGACCGCCTCACCATAGAGCGCAACCGCG
GGCTCCAGGCCCAGCGGGCCCTTCCCGAGCTCCGCGCCACCATCGCCGCCCTTCAGGCCCAAAGGCTCGCCTTCCTCCGG
GCCCTGCCCCGGGAGGAAAGGCTCGCCGAGGTCCTCTCCGAGGTGCTCCAGGACGCGGAGGCGAGCGGGGTGGTGGTCCG
AAGCTTCACCCGAAGCCGCACCTCGGCCCCGGTGCCCGAGGTGCGGGCCGTGAACCTGGCCCTGGCCCTCGAGGCCCCCT
TCCCCGAGACCTACGCCTACCTGCGGCGCCTGGAGGACCTCTCCCGCTTCAGCACCCTTTCCGGCCTCAACCTGAGCGTC
CAGAGCCAGGAAGCGAACCCCACCCTCGCCACCAGCCTCACCTTGACCGTCTACGTGCTGGCCCAGGGGGTGGAGGCGGA
AACGGGAGGGAGCACCCCATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(41-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Thermus thermophilus HB27

99.482

100

0.995

Multiple sequence alignment