Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ABWW24_RS03405 Genome accession   NZ_AP031330
Coordinates   590256..591389 (-) Length   377 a.a.
NCBI ID   WP_353300695.1    Uniprot ID   -
Organism   Thermus thermophilus strain TF25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 585256..596389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABWW24_RS03385 (TthTF25_06600) pilQ 585915..588188 (-) 2274 WP_353300693.1 secretin N-terminal domain-containing protein Machinery gene
  ABWW24_RS03390 (TthTF25_06610) pilW 588185..589072 (-) 888 WP_353300694.1 competence protein Machinery gene
  ABWW24_RS03395 (TthTF25_06620) pilO 589069..589650 (-) 582 WP_143586070.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABWW24_RS03400 (TthTF25_06630) pilN 589640..590263 (-) 624 WP_011173433.1 competence protein Machinery gene
  ABWW24_RS03405 (TthTF25_06640) pilM 590256..591389 (-) 1134 WP_353300695.1 type IV pilus assembly protein PilM Machinery gene
  ABWW24_RS03410 (TthTF25_06650) - 591526..592530 (-) 1005 WP_244340638.1 homoisocitrate dehydrogenase -
  ABWW24_RS03415 (TthTF25_06660) - 592585..593076 (-) 492 WP_011173430.1 DUF5317 domain-containing protein -
  ABWW24_RS03420 (TthTF25_06670) - 593127..594533 (-) 1407 WP_353300696.1 HD-GYP domain-containing protein -
  ABWW24_RS03435 (TthTF25_06680) - 595325..596014 (-) 690 WP_353386502.1 transposase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41383.02 Da        Isoelectric Point: 4.3805

>NTDB_id=108756 ABWW24_RS03405 WP_353300695.1 590256..591389(-) (pilM) [Thermus thermophilus strain TF25]
MFKSLSQLFRPRVEALGLEIGASALKLVEVSGNPPALKALASRPTPPGLLMEGMVAEPAALAQEIKELLLEARTRKRYVV
TALSNLAMILRSIQVPKMPLKEMEEAVRWEAERYIPFPIDEVVLDFAPLTPLSEVQEGEQVQVMVAAARQEAVAGVLEAL
RGAGLVPVVLDVKPFAGLYPLEARLAEEPDRVFLVLDIGAESTSLVLLRGDKPLAVRVLTLSGKDFTEAIARSFNLDLLA
AEEVKRTYGMATLPTEDEELLLDFDAERERYSPGRIYDAIRPVLVELTQELRRSLEFFRIQLEEAPPEVGYLLGGGSKLR
GLASLLTDTLGVNFEPVNPWEAVAVDPKRFEAEQLQEIGPEFAVALGLALRGVEPLD

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=108756 ABWW24_RS03405 WP_353300695.1 590256..591389(-) (pilM) [Thermus thermophilus strain TF25]
GTGTTCAAAAGCCTTAGCCAGCTCTTCCGACCCAGGGTTGAGGCCCTGGGGTTGGAGATCGGGGCCTCCGCCCTGAAGCT
CGTGGAGGTGTCCGGGAACCCCCCTGCCCTCAAGGCCCTGGCCTCCCGCCCCACCCCGCCCGGCCTCCTGATGGAGGGAA
TGGTGGCCGAGCCCGCCGCGCTGGCCCAGGAGATCAAGGAGCTTCTCCTCGAGGCCCGCACCCGCAAGCGCTACGTGGTC
ACCGCCCTCTCCAACCTGGCGATGATCCTCCGCTCCATCCAGGTCCCCAAGATGCCCCTCAAGGAGATGGAGGAAGCGGT
GCGCTGGGAGGCGGAGCGCTACATTCCCTTCCCCATTGACGAGGTGGTCCTGGACTTCGCCCCCCTGACCCCCCTCTCCG
AGGTGCAGGAGGGGGAGCAGGTCCAGGTGATGGTGGCGGCGGCGCGGCAGGAGGCGGTGGCGGGGGTCCTCGAGGCCCTG
AGGGGGGCGGGGCTCGTCCCCGTGGTGCTGGACGTGAAGCCCTTCGCCGGGCTTTACCCTCTGGAGGCCAGGCTTGCGGA
GGAGCCCGACCGGGTCTTCCTCGTCCTGGACATCGGGGCCGAGAGCACGAGCCTCGTCCTGCTCCGGGGGGACAAGCCCC
TGGCGGTGCGCGTCCTCACCCTCTCGGGCAAGGACTTCACCGAGGCCATCGCCCGAAGCTTCAACCTGGACCTCCTCGCC
GCCGAGGAGGTGAAGCGGACCTACGGGATGGCCACCCTCCCCACCGAGGACGAGGAACTCCTCCTGGACTTTGACGCCGA
GCGGGAGCGCTACAGCCCGGGGCGCATCTACGACGCCATCCGCCCGGTGCTGGTGGAGCTCACCCAGGAGCTCCGCCGTA
GCCTGGAGTTCTTCCGCATCCAGCTGGAGGAGGCCCCGCCCGAGGTGGGCTACCTCCTGGGCGGGGGGAGCAAGCTCAGG
GGGCTCGCCTCCCTCCTCACCGACACCCTGGGGGTGAACTTTGAGCCCGTCAACCCCTGGGAGGCGGTGGCGGTGGACCC
CAAGCGCTTTGAGGCGGAGCAACTCCAGGAGATCGGGCCGGAGTTCGCCGTGGCCCTGGGCTTGGCGTTGAGGGGGGTGG
AGCCCCTTGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

98.674

100

0.987

Multiple sequence alignment