Detailed information NTG1085969

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: ACJWMC_RS09640
• Genome accession: NZ_CP177181
• Coordinates: 2052048..2052485 (-)
• Length: 145 a.a.
• NCBI ID: WP_002887014.1
• Uniprot ID: A0A1V0GF29
• Organism: Streptococcus salivarius strain MRD1919
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2047048..2057485

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJWMC_RS09605	-	2047098..2047760 (-)	663	WP_037598525.1	CPBP family intramembrane glutamic endopeptidase	-
ACJWMC_RS09610	-	2047873..2048316 (-)	444	WP_002892029.1	hypothetical protein	-
ACJWMC_RS09615	-	2048390..2049046 (-)	657	WP_002892031.1	CPBP family intramembrane glutamic endopeptidase	-
ACJWMC_RS09620	-	2049058..2049255 (-)	198	WP_037598528.1	helix-turn-helix transcriptional regulator	-
ACJWMC_RS09625	-	2049502..2050695 (-)	1194	WP_002892033.1	acetate kinase	-
ACJWMC_RS09630	comYH	2050752..2051708 (-)	957	WP_002885753.1	class I SAM-dependent methyltransferase	Machinery gene
ACJWMC_RS09635	comGG	2051753..2052070 (-)	318	WP_021144683.1	competence type IV pilus minor pilin ComGG	-
ACJWMC_RS09640	comYF	2052048..2052485 (-)	438	WP_002887014.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACJWMC_RS09645	comGE	2052472..2052702 (-)	231	WP_014635119.1	competence type IV pilus minor pilin ComGE	-
ACJWMC_RS09650	comYD	2052734..2053162 (-)	429	WP_260312930.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACJWMC_RS09655	comYC	2053122..2053436 (-)	315	WP_037611125.1	competence type IV pilus major pilin ComGC	Machinery gene
ACJWMC_RS09660	comYB	2053445..2054545 (-)	1101	WP_155115607.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACJWMC_RS09665	comYA	2054427..2055368 (-)	942	WP_002885658.1	competence type IV pilus ATPase ComGA	Machinery gene
ACJWMC_RS09670	-	2055448..2055810 (-)	363	WP_002885749.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16155.57 Da
• Isoelectric Point: 8.4658

>NTDB_id=1085969 ACJWMC_RS09640 WP_002887014.1 2052048..2052485(-) (comYF) [Streptococcus salivarius strain MRD1919]
MSLNSKVRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVKQQESQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSATDFRKTNADGRGYQPMLFNVKASTISQNNQIVTIQVTLKNGLQRSFVYAFEKTG

Nucleotide

• Length: 438 bp

>NTDB_id=1085969 ACJWMC_RS09640 WP_002887014.1 2052048..2052485(-) (comYF) [Streptococcus salivarius strain MRD1919]
ATGTCTCTCAATAGTAAGGTTCGCGCCTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTGATTGCTGGTTCAGTTCA
AGTTTATCAAGGTCTGACTACAGTATTGGTGAGCAATGTTAAGCAGGTCAAGCAACAAGAAAGCCAAGACTGGCTTCTAT
TCGTTCAACAAATGGAGGCAGAATTAGAAGGTTGTCAGTTGGTAAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACCTGGCTTTTGGACTTTCAAGTGCTACTGATTTTCGAAAAACAAATGCTGATGGTCGAGGCTATCAACCAAT
GCTTTTCAATGTAAAAGCTAGTACAATTAGCCAAAATAACCAGATAGTCACCATCCAAGTGACTCTTAAAAATGGGCTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A1V0GF29

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	58.156	97.241	0.566
comYF	Streptococcus mutans UA159	57.447	97.241	0.559
comGF/cglF	Streptococcus pneumoniae Rx1	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae D39	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae R6	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae TIGR4	50	97.931	0.49
comGF/cglF	Streptococcus mitis SK321	47.222	99.31	0.469
comGF/cglF	Streptococcus mitis NCTC 12261	47.222	99.31	0.469
comGF	Lactococcus lactis subsp. cremoris KW2	45.324	95.862	0.434