Detailed information NTG1080793

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACK2WG_RS12730
• Genome accession: NZ_CP176536
• Coordinates: 2508256..2508873 (-)
• Length: 205 a.a.
• NCBI ID: WP_409509004.1
• Uniprot ID: -
• Organism: Bacillus spizizenii strain 29-4
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2503256..2513873

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK2WG_RS12700 (ACK2WG_12700)	rpsT	2503557..2503823 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ACK2WG_RS12705 (ACK2WG_12705)	holA	2503838..2504881 (-)	1044	WP_061186855.1	DNA polymerase III subunit delta	-
ACK2WG_RS12710 (ACK2WG_12710)	-	2504921..2505070 (-)	150	WP_003229985.1	hypothetical protein	-
ACK2WG_RS12715 (ACK2WG_12715)	-	2505111..2505245 (+)	135	WP_003229983.1	YqzM family protein	-
ACK2WG_RS12720 (ACK2WG_12720)	comEC	2505286..2507616 (-)	2331	WP_061186854.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACK2WG_RS12725 (ACK2WG_12725)	-	2507620..2508189 (-)	570	WP_061186853.1	ComE operon protein 2	-
ACK2WG_RS12730 (ACK2WG_12730)	comEA	2508256..2508873 (-)	618	WP_409509004.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACK2WG_RS12735 (ACK2WG_12735)	comER	2508957..2509778 (+)	822	WP_061186851.1	late competence protein ComER	-
ACK2WG_RS12740 (ACK2WG_12740)	-	2509844..2510587 (-)	744	WP_014114475.1	class I SAM-dependent DNA methyltransferase	-
ACK2WG_RS12745 (ACK2WG_12745)	rsfS	2510584..2510940 (-)	357	WP_003237112.1	ribosome silencing factor	-
ACK2WG_RS12750 (ACK2WG_12750)	yqeK	2510958..2511518 (-)	561	WP_061186850.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACK2WG_RS12755 (ACK2WG_12755)	-	2511508..2512077 (-)	570	WP_087986864.1	nicotinate-nucleotide adenylyltransferase	-
ACK2WG_RS12760 (ACK2WG_12760)	yhbY	2512089..2512379 (-)	291	WP_014114478.1	ribosome assembly RNA-binding protein YhbY	-
ACK2WG_RS12765 (ACK2WG_12765)	aroE	2512373..2513215 (-)	843	WP_242734039.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 22087.00 Da
• Isoelectric Point: 4.8542

>NTDB_id=1080793 ACK2WG_RS12730 WP_409509004.1 2508256..2508873(-) (comEA) [Bacillus spizizenii strain 29-4]
MNWLNQHKKAIILAASAAVFIAVIFFLASGKNKEPMKQTVSTETENTEVKQEAFKDESNETIVIDIKGAVKHPGVYEMRT
GDRLSQAIEKAGGTSEQADEMQVNLAELLQDGTVVYIPKRGEETAVQQGIGGAIQSDGGKGALVNINTATLEELQGISGM
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1080793 ACK2WG_RS12730 WP_409509004.1 2508256..2508873(-) (comEA) [Bacillus spizizenii strain 29-4]
ATGAATTGGTTGAACCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCATAGCGGTTATTTTCTTCCT
GGCCAGTGGGAAAAACAAAGAGCCGATGAAGCAGACTGTATCAACTGAGACAGAAAATACGGAGGTAAAGCAGGAAGCAT
TCAAAGACGAGTCAAACGAAACGATTGTGATTGACATCAAAGGTGCTGTTAAGCATCCTGGTGTTTATGAAATGCGAACA
GGGGACAGATTATCTCAGGCGATTGAGAAAGCCGGCGGGACCAGTGAACAAGCAGACGAAATGCAAGTGAATTTGGCGGA
GCTGCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAGGGGTGAGGAGACAGCGGTGCAGCAAGGAATCGGAGGCGCCA
TCCAAAGCGATGGAGGGAAGGGGGCGCTGGTAAACATCAATACAGCAACATTAGAGGAATTACAAGGCATCTCAGGGATG
GGGCCATCCAAAGCTGAAGCTATTATTGCATATCGGGAAGAAAATGGGCGTTTCCAGACAATTGAAGATATCACGAAGGT
TTCAGGAATCGGTGAAAAGTCGTTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	90.244	100	0.902
comEA	Staphylococcus aureus MW2	36.406	100	0.385
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	37.264	100	0.385
comEA	Staphylococcus aureus N315	34.562	100	0.366