Detailed information NTG1079526

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACKPXQ_RS12310
• Genome accession: NZ_CP176038
• Coordinates: 2553028..2553642 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain A4
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2548028..2558642

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACKPXQ_RS12280 (ACKPXQ_12280)	rpsT	2548331..2548597 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACKPXQ_RS12285 (ACKPXQ_12285)	holA	2548560..2549655 (-)	1096	Protein_2372	DNA polymerase III subunit delta	-
ACKPXQ_RS12290 (ACKPXQ_12290)	-	2549695..2549846 (-)	152	Protein_2373	hypothetical protein	-
ACKPXQ_RS12295 (ACKPXQ_12295)	-	2549887..2550021 (+)	135	WP_003152870.1	YqzM family protein	-
ACKPXQ_RS12300 (ACKPXQ_12300)	comEC	2550040..2552391 (-)	2352	WP_182071847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACKPXQ_RS12305 (ACKPXQ_12305)	-	2552392..2552961 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACKPXQ_RS12310 (ACKPXQ_12310)	comEA	2553028..2553642 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACKPXQ_RS12315 (ACKPXQ_12315)	comER	2553701..2554522 (+)	822	WP_012118027.1	late competence protein ComER	-
ACKPXQ_RS12320 (ACKPXQ_12320)	-	2554591..2555328 (-)	738	WP_167408922.1	class I SAM-dependent methyltransferase	-
ACKPXQ_RS12325 (ACKPXQ_12325)	rsfS	2555325..2555681 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACKPXQ_RS12330 (ACKPXQ_12330)	yqeK	2555699..2556259 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACKPXQ_RS12335 (ACKPXQ_12335)	-	2556249..2556818 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
ACKPXQ_RS12340 (ACKPXQ_12340)	yhbY	2556829..2557119 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACKPXQ_RS12345 (ACKPXQ_12345)	aroE	2557113..2557955 (-)	843	WP_182071849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=1079526 ACKPXQ_RS12310 WP_014418419.1 2553028..2553642(-) (comEA) [Bacillus velezensis strain A4]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1079526 ACKPXQ_RS12310 WP_014418419.1 2553028..2553642(-) (comEA) [Bacillus velezensis strain A4]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363