Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACJD0W_RS13620 Genome accession   NZ_CP174456
Coordinates   2985302..2985895 (-) Length   197 a.a.
NCBI ID   WP_038885610.1    Uniprot ID   -
Organism   Vibrio rotiferianus strain BD182406     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2980302..2990895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJD0W_RS13600 aroB 2981186..2982286 (-) 1101 WP_407832519.1 3-dehydroquinate synthase -
  ACJD0W_RS13605 aroK 2982318..2982836 (-) 519 WP_010443904.1 shikimate kinase AroK -
  ACJD0W_RS13610 pilQ 2983026..2984759 (-) 1734 WP_354419523.1 type IV pilus secretin PilQ family protein Machinery gene
  ACJD0W_RS13615 pilP 2984797..2985312 (-) 516 WP_010443909.1 pilus assembly protein PilP Machinery gene
  ACJD0W_RS13620 pilO 2985302..2985895 (-) 594 WP_038885610.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACJD0W_RS13625 pilN 2985892..2986476 (-) 585 WP_010443929.1 PilN domain-containing protein Machinery gene
  ACJD0W_RS13630 pilM 2986460..2987476 (-) 1017 WP_407832520.1 type IV pilus assembly protein PilM Machinery gene
  ACJD0W_RS13635 - 2987616..2990183 (+) 2568 WP_407832521.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 22770.30 Da        Isoelectric Point: 5.7550

>NTDB_id=1077138 ACJD0W_RS13620 WP_038885610.1 2985302..2985895(-) (pilO) [Vibrio rotiferianus strain BD182406]
MSNWQELELDEITDWPLLPQSLVVCLLVIVLLAGGYWAFVKPDKQRLNSLIAEEQKLKVMLKIKANKVAALPKLQAQLDE
LTHRYEYLLQQLPAQKELATMLASVNELGIDNNLTFTRIDWGKKQSEQFLYRLPLNIELTGAFHDIGSFAQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKQDES

Nucleotide


Download         Length: 594 bp        

>NTDB_id=1077138 ACJD0W_RS13620 WP_038885610.1 2985302..2985895(-) (pilO) [Vibrio rotiferianus strain BD182406]
ATGAGCAACTGGCAAGAACTAGAATTAGATGAAATAACAGATTGGCCTCTATTACCCCAGTCTTTGGTCGTTTGTTTACT
GGTAATAGTTCTACTAGCAGGGGGCTATTGGGCTTTTGTTAAGCCGGATAAACAGCGTTTGAACTCGTTAATCGCCGAAG
AGCAGAAACTCAAAGTAATGTTGAAAATCAAAGCCAATAAGGTCGCAGCATTACCCAAACTGCAAGCCCAATTGGATGAA
CTGACTCATCGATATGAATATCTATTGCAGCAATTACCAGCACAGAAAGAGCTGGCCACAATGCTTGCTTCTGTCAACGA
ACTTGGCATCGATAATAATCTGACTTTCACACGGATCGATTGGGGGAAGAAACAATCTGAACAATTTTTGTATAGGCTCC
CATTGAATATCGAACTTACTGGCGCATTTCATGATATTGGGAGCTTCGCACAAGCGATAGCAACGCTTCCTCGCATCATT
ACATTTAAGGATGTTACTTGGCAGAGAGTCAGTCAAGAGAGTGAAACCTTACATTTTCGAGTTAGAGCCAATACTTATCA
GTTTAAGCCAGAGGAAAAACAAGATGAAAGCTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

78.421

96.447

0.756
  pilO Vibrio cholerae strain A1552

65.445

96.954

0.635

Multiple sequence alignment