Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACJD0W_RS13630 Genome accession   NZ_CP174456
Coordinates   2986460..2987476 (-) Length   338 a.a.
NCBI ID   WP_407832520.1    Uniprot ID   -
Organism   Vibrio rotiferianus strain BD182406     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2981460..2992476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJD0W_RS13605 aroK 2982318..2982836 (-) 519 WP_010443904.1 shikimate kinase AroK -
  ACJD0W_RS13610 pilQ 2983026..2984759 (-) 1734 WP_354419523.1 type IV pilus secretin PilQ family protein Machinery gene
  ACJD0W_RS13615 pilP 2984797..2985312 (-) 516 WP_010443909.1 pilus assembly protein PilP Machinery gene
  ACJD0W_RS13620 pilO 2985302..2985895 (-) 594 WP_038885610.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACJD0W_RS13625 pilN 2985892..2986476 (-) 585 WP_010443929.1 PilN domain-containing protein Machinery gene
  ACJD0W_RS13630 pilM 2986460..2987476 (-) 1017 WP_407832520.1 type IV pilus assembly protein PilM Machinery gene
  ACJD0W_RS13635 - 2987616..2990183 (+) 2568 WP_407832521.1 penicillin-binding protein 1A -
  ACJD0W_RS13640 oxyR 2990262..2991170 (-) 909 WP_010443932.1 DNA-binding transcriptional regulator OxyR -
  ACJD0W_RS13645 - 2991345..2992073 (+) 729 WP_010443933.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38271.70 Da        Isoelectric Point: 6.2468

>NTDB_id=1077140 ACJD0W_RS13630 WP_407832520.1 2986460..2987476(-) (pilM) [Vibrio rotiferianus strain BD182406]
MDKLTVAGIDISHNSLKAVILKPAKEGYSLLGYKEIVLNDAIVAENHTINHQEIVKTLKEIKKELPLLHRKAAASIPDSA
VISKSLQLDSQLEESEVEFAIMQAFSLQSPFPIEELSLDFVPNCDSKGNEVAGFRVFATRKEVVESRKQAIEKAGLTPIL
LDVHSHSQGAIWQLATERHPEKAQYCLLYVGERNCSISMFTAQGEFFYKEFSSGLEQVINNFQTSLEENASEQEKVERFN
HETADRVKRQLQLYSSMNGERDIRGIWLVGEGASTLMLAETLERQLQLDCELLNPFSLFEMKVPKRKRRNVDWQRFVTAA
GIAIRAIDWQRGIHVASR

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1077140 ACJD0W_RS13630 WP_407832520.1 2986460..2987476(-) (pilM) [Vibrio rotiferianus strain BD182406]
ATGGATAAGTTAACAGTTGCGGGCATTGATATTAGTCACAATAGCCTAAAAGCCGTAATACTAAAGCCAGCAAAGGAGGG
GTACTCCTTGCTAGGTTATAAAGAAATAGTGCTAAACGACGCTATTGTCGCTGAAAACCATACAATAAATCATCAAGAAA
TTGTAAAGACCCTAAAAGAGATAAAAAAAGAACTACCACTATTACACCGAAAAGCGGCTGCATCTATTCCAGATAGCGCT
GTAATTAGCAAAAGCTTACAGCTTGACTCTCAATTGGAAGAAAGTGAGGTTGAGTTTGCAATCATGCAGGCATTTTCTCT
TCAGTCTCCGTTTCCTATTGAGGAATTAAGTTTAGATTTTGTGCCGAACTGTGACAGCAAAGGAAATGAGGTTGCTGGTT
TTCGAGTCTTTGCTACTAGAAAAGAGGTGGTAGAAAGTCGAAAACAGGCAATTGAAAAGGCTGGCCTGACACCTATTTTG
CTTGACGTTCACTCTCACTCTCAGGGAGCAATATGGCAATTGGCGACAGAGCGGCATCCTGAAAAAGCGCAATACTGTTT
GCTTTATGTGGGAGAAAGAAACTGTTCTATTTCCATGTTTACCGCTCAAGGTGAGTTCTTTTATAAGGAGTTTTCATCAG
GCTTAGAGCAAGTTATCAATAACTTTCAAACATCTCTTGAGGAGAATGCGAGCGAACAAGAGAAAGTGGAAAGGTTTAAC
CATGAAACGGCAGATAGGGTAAAGAGACAGCTTCAACTTTACAGCTCAATGAATGGTGAACGAGATATTCGTGGTATTTG
GCTTGTCGGTGAAGGCGCATCCACTTTAATGCTCGCAGAAACGTTGGAGCGTCAGTTACAACTGGATTGTGAATTGTTGA
ATCCATTTAGTCTTTTTGAAATGAAGGTTCCGAAGCGTAAAAGAAGAAATGTTGACTGGCAGCGTTTTGTAACTGCGGCT
GGTATAGCAATAAGAGCTATTGACTGGCAAAGGGGGATCCATGTTGCATCCCGTTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

59.587

100

0.598
  pilM Vibrio cholerae strain A1552

45.349

100

0.462

Multiple sequence alignment