Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJEH2_RS15000 Genome accession   NZ_CP174235
Coordinates   3351265..3352989 (-) Length   574 a.a.
NCBI ID   WP_011215550.1    Uniprot ID   Q5WWE9
Organism   Legionella pneumophila strain SPF590     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3346265..3357989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEH2_RS14975 (ACJEH2_14975) - 3346739..3347194 (+) 456 WP_011215546.1 hypothetical protein -
  ACJEH2_RS14980 (ACJEH2_14980) - 3347210..3347803 (+) 594 WP_011213808.1 hypothetical protein -
  ACJEH2_RS14985 (ACJEH2_14985) - 3347911..3349116 (+) 1206 WP_011215547.1 cyclic nucleotide-binding domain-containing protein -
  ACJEH2_RS14990 (ACJEH2_14990) pilD 3349130..3349993 (-) 864 WP_011215548.1 A24 family peptidase Machinery gene
  ACJEH2_RS14995 (ACJEH2_14995) pilC 3350039..3351259 (-) 1221 WP_011215549.1 type II secretion system F family protein Machinery gene
  ACJEH2_RS15000 (ACJEH2_15000) pilB 3351265..3352989 (-) 1725 WP_011215550.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJEH2_RS15005 (ACJEH2_15005) - 3353248..3353991 (+) 744 WP_011215551.1 methyltransferase domain-containing protein -
  ACJEH2_RS15010 (ACJEH2_15010) - 3354174..3354500 (+) 327 WP_011213802.1 DUF1820 family protein -
  ACJEH2_RS15015 (ACJEH2_15015) - 3354543..3355112 (+) 570 WP_011215552.1 hypoxanthine-guanine phosphoribosyltransferase -
  ACJEH2_RS15020 (ACJEH2_15020) - 3355114..3357636 (-) 2523 WP_011215553.1 bifunctional diguanylate cyclase/phosphodiesterase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62676.18 Da        Isoelectric Point: 5.5861

>NTDB_id=1076115 ACJEH2_RS15000 WP_011215550.1 3351265..3352989(-) (pilB) [Legionella pneumophila strain SPF590]
MALATEEYRLQGIGQLLVLEKMLDKAKAIELHKLAAAEKMSLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTI
PANLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDSDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKESDLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1076115 ACJEH2_RS15000 WP_011215550.1 3351265..3352989(-) (pilB) [Legionella pneumophila strain SPF590]
ATGGCTCTAGCTACAGAAGAATATAGATTACAGGGAATCGGCCAGCTTCTCGTGCTGGAAAAGATGCTAGATAAAGCAAA
AGCAATTGAACTACACAAGCTGGCAGCAGCAGAAAAGATGTCTTTGCTGCAATACATTGTAAAAAATAAAATATTATCTG
CTGAACAAATTGCACTGACAGCTGCTCAAAATTTTGGCGTACCCATGTTGGATATTAACTGCATTGATGTAGGCACCATT
CCTGCCAATCTGGTTAATGAGAAATTAATTAAACGTCATGCCATGGTTCCTCTTTTTAGTCGCGGTACCAATTTATATCT
CGCAACAGATGATCCTAGTAAACAAGCTTCATTAAAGGAAATTCAATTTCACACCGGATTAAATACTCATGCGATAGTTG
TAGAAACAGATAAACTTAGTGCCCTGATTGATAACCTGTTAACAGCAAAGGAAAGTCAGGGCTTATCAGAGTATGTTGAA
GACTCTGGAGATCTGGAAGGTTTAGAAATAAGTGCTGATGATGAAGATCAAGATAGTGATACTGCAACATCAGTAACTGA
TGATGCGCCTATCGTAAAATTCGTCAATAAAATTTTATTGGATGCGATAAGGCAAGGAGCTTCTGATATACACTTTGAGC
CTTATGAACGAGAATACCGAATCAGATACAGACAAGATGGTATTTTACATGAAGTCGCTACCCCTCCCGCAAGCTTGTCA
TCTCGTATTACAGCACGTATCAAGGTAATGTCTAATTTGGATATATCAGAACGGCGTATCCCCCAGGATGGTGGCTTCAA
AATGAAAATTTCCAAGTCACGAGCAATTGATTTCAGAGTGAGTACATGTCCAACTTCGGCTGGGGAAAAAGTGGTTATGC
GGGTTCTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTGGGATTTAATCCTGTTCAGAGAACAAATTTCCTGAAA
GCAATCCAGCGCCCACAAGGGATGATTTTAGTCACTGGCCCTACTGGTAGTGGTAAAACAGTGACTTTATATACGGCTTT
AAATATATTAAATACAATTGAGGTTAATATTTCAACAGCTGAAGATCCAGTTGAAATCAAAGTTCCTGGTATCAATCAGG
TTAATATTAATCCTAAAGCTGGCCTAACCTTTTCCGGTGCATTACGTTCGTTTCTAAGGCAAGACCCTGATATTATAATG
GTTGGTGAGATACGAGACCTTGAAACAGCAGAGATTGCTGTAAAAGCTGCTCAGACAGGGCATTTGGTTCTTTCTACTCT
GCACACAAACAGTGCCGCTGAAACTTTAAATCGTTTAGTAAACATGGGAATACCAACTTTTAACATTGCGAGTTCGGTTA
CCTTAATTATTGCGCAAAGGCTGGCAAGAAAATTATGCAATCAATGTAAGGCAGTGCGAGATGACTTTACCAATCAAGGT
TTGATTGAACTAGGTTTCAAGGAATCTGATCTGGTTAATCTTAAATTATACAAAGCAGTTGGTTGCGAGCAATGTACAAG
TGGTTATCGCGGACGAGTCGGGTTGTTTGAGGTTTTACCGATGACGAAAGAGCTTGGCCAATTGATTATGTCAGGTGGTA
ATTCGCTGGATATATTAAAGTTAGCTCAATCAGAAGGTATGCTGACTATTTTTCAATCGGGTATAGAAAAAGTTAAGGAA
GGAATCACGACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5WWE9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

98.78

100

0.988
  pilB Acinetobacter baumannii D1279779

50.794

98.78

0.502
  pilB Acinetobacter baylyi ADP1

49.22

100

0.495
  pilB Vibrio cholerae strain A1552

50.741

94.077

0.477
  pilF Neisseria gonorrhoeae MS11

46.996

98.606

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

48.609

93.902

0.456
  pilB Vibrio campbellii strain DS40M4

47.963

94.077

0.451
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.811

92.334

0.368
  pilF Thermus thermophilus HB27

38.476

93.728

0.361

Multiple sequence alignment