Detailed information NTG1075799

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACJEHG_RS04890
• Genome accession: NZ_CP174228
• Coordinates: 1111304..1112368 (-)
• Length: 354 a.a.
• NCBI ID: WP_010946662.1
• Uniprot ID: Q5ZX06
• Organism: Legionella pneumophila strain SPF638
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1106304..1117368

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJEHG_RS04865 (ACJEHG_04865)	aroK	1106482..1107009 (-)	528	WP_010946667.1	shikimate kinase AroK	-
ACJEHG_RS04870 (ACJEHG_04870)	pilQ	1107455..1109551 (-)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
ACJEHG_RS04875 (ACJEHG_04875)	pilP	1109555..1110142 (-)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
ACJEHG_RS04880 (ACJEHG_04880)	pilO	1110139..1110738 (-)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACJEHG_RS04885 (ACJEHG_04885)	pilN	1110752..1111300 (-)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
ACJEHG_RS04890 (ACJEHG_04890)	pilM	1111304..1112368 (-)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
ACJEHG_RS04895 (ACJEHG_04895)	ravI	1112785..1113795 (+)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
ACJEHG_RS04900 (ACJEHG_04900)	-	1113947..1116331 (+)	2385	WP_010946660.1	penicillin-binding protein 1A	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38732.71 Da
• Isoelectric Point: 5.0367

>NTDB_id=1075799 ACJEHG_RS04890 WP_010946662.1 1111304..1112368(-) (pilM) [Legionella pneumophila strain SPF638]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQSGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=1075799 ACJEHG_RS04890 WP_010946662.1 1111304..1112368(-) (pilM) [Legionella pneumophila strain SPF638]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATCCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGCGAAGAAATACCTGCCTCTGCCTTAGAAGGAAATATAA
TTAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATCGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAATCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5ZX06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.435	100	0.994
comM	Acinetobacter nosocomialis M2	42.817	100	0.429
pilM	Acinetobacter baumannii D1279779	42.535	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418