Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACJBUE_RS08535 Genome accession   NZ_CP174148
Coordinates   1762727..1764454 (-) Length   575 a.a.
NCBI ID   WP_078222783.1    Uniprot ID   -
Organism   Ralstonia syzygii subsp. celebesensis strain UGMSLYSS_Db01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1757727..1769454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJBUE_RS08505 (ACJBUE_08505) - 1758349..1758807 (+) 459 WP_048817320.1 NUDIX domain-containing protein -
  ACJBUE_RS08510 (ACJBUE_08510) - 1758804..1759019 (-) 216 WP_013211401.1 DNA gyrase inhibitor YacG -
  ACJBUE_RS08515 (ACJBUE_08515) zapD 1759031..1759789 (-) 759 WP_013211402.1 cell division protein ZapD -
  ACJBUE_RS08520 (ACJBUE_08520) coaE 1759906..1760520 (-) 615 WP_078222780.1 dephospho-CoA kinase -
  ACJBUE_RS08525 (ACJBUE_08525) pilD 1760533..1761414 (-) 882 WP_078222781.1 A24 family peptidase Machinery gene
  ACJBUE_RS08530 (ACJBUE_08530) - 1761417..1762682 (-) 1266 WP_078222782.1 type II secretion system F family protein -
  ACJBUE_RS08535 (ACJBUE_08535) pilF 1762727..1764454 (-) 1728 WP_078222783.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJBUE_RS08540 (ACJBUE_08540) - 1764700..1765977 (-) 1278 WP_013211407.1 HlyC/CorC family transporter -
  ACJBUE_RS08550 (ACJBUE_08550) ispB 1766256..1767222 (-) 967 Protein_1680 octaprenyl diphosphate synthase -
  ACJBUE_RS08555 (ACJBUE_08555) rplU 1767501..1767812 (+) 312 WP_003271629.1 50S ribosomal protein L21 -
  ACJBUE_RS08560 (ACJBUE_08560) rpmA 1767850..1768110 (+) 261 WP_004633021.1 50S ribosomal protein L27 -
  ACJBUE_RS08565 (ACJBUE_08565) obgE 1768297..1769397 (+) 1101 WP_013211409.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63642.39 Da        Isoelectric Point: 7.4490

>NTDB_id=1074544 ACJBUE_RS08535 WP_078222783.1 1762727..1764454(-) (pilF) [Ralstonia syzygii subsp. celebesensis strain UGMSLYSS_Db01]
MTLGLALAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDAVPAVLSTNKHF
QQLRLLPLGRRDNRIIVATSDPSDPKPIEELRQKMNVAIETVIVEHDKLVRQLSLANEAPTEIKSLIPKQEATQIEYDPG
AAAAVRRTTTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILAQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKETIERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLTNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPRETL
LEAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILSHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1074544 ACJBUE_RS08535 WP_078222783.1 1762727..1764454(-) (pilF) [Ralstonia syzygii subsp. celebesensis strain UGMSLYSS_Db01]
ATGACTCTCGGACTCGCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCACCCAGCTTGAACAAAGCGCACGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCGCACGATCTCGCCGTCTTCGTGGCGGGCA
AGTACCAGATGCCGCTGCTGGACCTGGCGCAGTACAAGCTCGATGCCGTGCCGGCCGTGCTGTCGACCAACAAGCATTTC
CAGCAATTGCGCCTGTTGCCGCTGGGCCGGCGCGACAACCGCATCATCGTTGCCACCTCGGACCCGAGCGACCCCAAGCC
GATCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCGCCAACGAAGCGCCGACCGAGATCAAGTCGCTCATCCCCAAGCAGGAAGCCACGCAGATCGAATACGACCCGGGC
GCAGCCGCTGCAGTGCGGCGTACCACGACCGACGGCATCGACGATGCGCCGGTGGTGCGTTTCCTGCAAAAGCTGTTTAC
CGAAGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGTATCGACG
GCATCCTCGCGCAGGTCGCGCAGCCACCGCTGGATATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATCTCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACGGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGACGATCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCCACCGAGCGGCTCGATATCGACCAGCTAGGCTATGAGCCCGAGCAAAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAAACGGTCTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAAGCGGGGCTGACCTTCGCCGCGGCGCTGAAATCGTTCCTGCGGCAGGATCCGGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGATGCGCCGACCACGCTCACGCGGCTGACGAACATGGGCGTGGCACCGTTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCATGCAAGAAGCCGGGCCCGCTGCCCCGGGAAACACTG
CTGGAGGCCGGCTTCAAGGAAGCCGACCTGGACGGCTCGTGGCAGCCGTACCATCCAGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCAGCCACG
GCACGGCACTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCTGGCCTGCGGAAAGTGAAA
CAGGGGCTGACTTCCCTTGAGGAAGTGCTGGCCACGACGAACCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.519

93.913

0.503
  pilB Acinetobacter baumannii D1279779

51.15

98.261

0.503
  pilB Acinetobacter baylyi ADP1

50.089

97.913

0.49
  pilB Legionella pneumophila strain ERS1305867

46.585

99.304

0.463
  pilB Vibrio cholerae strain A1552

44.784

96.696

0.433
  pilB Vibrio campbellii strain DS40M4

44.834

94.261

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

44.649

94.261

0.421
  pilF Thermus thermophilus HB27

37.699

98.261

0.37

Multiple sequence alignment