Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACI703_RS04575 Genome accession   NZ_CP173765
Coordinates   1031754..1033481 (+) Length   575 a.a.
NCBI ID   WP_406234977.1    Uniprot ID   -
Organism   Isoptericola jiangsuensis strain JSH01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1026754..1038481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI703_RS04560 (ACI703_04560) - 1028572..1029018 (+) 447 WP_406234971.1 GtrA family protein -
  ACI703_RS04565 (ACI703_04565) - 1029015..1030559 (+) 1545 WP_406234973.1 NAD(P)/FAD-dependent oxidoreductase -
  ACI703_RS04570 (ACI703_04570) galE 1030662..1031639 (+) 978 WP_406234975.1 UDP-glucose 4-epimerase GalE -
  ACI703_RS04575 (ACI703_04575) pilB 1031754..1033481 (+) 1728 WP_406234977.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACI703_RS04580 (ACI703_04580) pilR 1033649..1035022 (-) 1374 WP_406234979.1 sigma-54-dependent transcriptional regulator Regulator
  ACI703_RS04585 (ACI703_04585) - 1035078..1036691 (-) 1614 WP_406234981.1 sensor histidine kinase -
  ACI703_RS04590 (ACI703_04590) sucC 1037116..1038285 (+) 1170 WP_079222939.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62495.65 Da        Isoelectric Point: 6.3014

>NTDB_id=1073976 ACI703_RS04575 WP_406234977.1 1031754..1033481(+) (pilB) [Isoptericola jiangsuensis strain JSH01]
MNAVTTANLVGITGLARRLVQDGALDEAAARDAMAKAAVAKQPLPTWFAQNKLVSAAQLAAANSVEFGMPLFDVSTFDAS
QNAMTLVSEELLRKHHVLPLFKRGGRLFVGTSNPTHSLDEIKFHTNLVVEPILIDEDQIRRTLEQWHASHDTMSDALGGD
DEGMANLDISGGDEDMASTDSGVDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFL
EAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRLTNLPAHA
LLAEGFTQEQLDAGIQLYEAVGCDECTEGYKGRTGIYQVMPMTDEISTIILAGGNALQIAEAAQAIKVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1073976 ACI703_RS04575 WP_406234977.1 1031754..1033481(+) (pilB) [Isoptericola jiangsuensis strain JSH01]
ATGAACGCCGTAACCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGCCGCTTGGTCCAGGATGGCGCGCTGGATGA
GGCTGCGGCCCGGGACGCCATGGCCAAGGCCGCCGTGGCCAAGCAGCCGCTGCCGACCTGGTTCGCCCAGAACAAGCTGG
TCAGTGCCGCTCAGCTGGCCGCAGCCAATTCGGTCGAGTTCGGCATGCCGTTGTTCGACGTATCCACGTTCGATGCCAGC
CAGAACGCGATGACCCTGGTCAGCGAAGAGCTGCTGCGCAAGCACCACGTGCTGCCGCTGTTCAAGCGCGGCGGTCGGTT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGATGAGATCAAGTTCCATACCAACCTGGTGGTCGAGCCAATCCTGA
TCGACGAGGACCAGATCAGGCGCACCCTGGAACAGTGGCACGCCAGCCACGACACCATGTCCGACGCCCTGGGTGGTGAC
GACGAGGGCATGGCCAACCTCGACATCTCCGGCGGTGACGAGGACATGGCTTCGACCGACAGCGGCGTCGACGCCAAGGG
CGACGACACCCCGGTGGTCAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCCTCGGACATCCATTTCG
AGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTCGCGCGCGCGCCGGTCAAGTTG
AACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAAAAGCGCGTGCCGCAGGACGGACGCAT
CAAGCTGAATCTCTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCTTGCCCACGCTGTTCGGCGAGAAGGTCGTGC
TGCGTATCCTGGATGGCAGCGCGGCCAAGCTCGGCATCGACAAGTTGGGTTACGAGCCCGACCAGCAGAAGCTGTTCCTG
GAGGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGTAAGACCGTGTCGCTGTACACCGC
GCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACTGCGGAAGACCCGGTGGAAATCCGTCTGCCCGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTTGCGGCCGCGCTGCGCTCGTTTCTGCGACAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTTGAAACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGTCACATGGTGCTGTCCAC
GCTGCATACCAACGACGCACCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCTCGG
TGACCCTGGTGATCGCCCAGCGACTGGCCCGTCGCCTGTGCAGCAACTGCAAGCGGCTGACCAATCTGCCGGCTCATGCG
CTGCTGGCCGAAGGCTTCACCCAGGAGCAGCTGGATGCGGGCATCCAGCTGTACGAAGCGGTGGGCTGCGACGAGTGCAC
CGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCTCGACGATCATCCTGGCCGGTG
GTAATGCGCTGCAGATTGCCGAGGCGGCACAGGCGATCAAGGTGAATGACCTGCGCCAGTCGGCACTGAAGAAGGCAGCG
GCGGGCGTGACCAGCCTGGCCGAGATCAACCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.055

100

0.563
  pilB Acinetobacter baumannii D1279779

56.085

98.609

0.553
  pilB Legionella pneumophila strain ERS1305867

51.585

98.783

0.51
  pilF Neisseria gonorrhoeae MS11

49.206

98.609

0.485
  pilB Vibrio cholerae strain A1552

50.093

93.043

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

45.455

99.478

0.452
  pilB Vibrio campbellii strain DS40M4

44.056

99.478

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.462

94.957

0.365

Multiple sequence alignment