Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   ACI515_RS20255 Genome accession   NZ_CP173628
Coordinates   4471416..4472942 (+) Length   508 a.a.
NCBI ID   WP_405078589.1    Uniprot ID   -
Organism   Pectobacterium carotovorum strain M3     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 4466416..4477942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI515_RS20235 (ACI515_20230) - 4467832..4468758 (-) 927 WP_014916970.1 branched-chain amino acid transaminase -
  ACI515_RS20240 (ACI515_20235) ilvM 4468784..4469044 (-) 261 WP_010294673.1 acetolactate synthase 2 small subunit -
  ACI515_RS20245 (ACI515_20240) ilvG 4469123..4470769 (-) 1647 WP_039492865.1 acetolactate synthase 2 catalytic subunit -
  ACI515_RS20250 (ACI515_20245) ilvL 4470910..4471008 (-) 99 WP_071531109.1 ilv operon leader peptide -
  ACI515_RS20255 (ACI515_20250) comM 4471416..4472942 (+) 1527 WP_405078589.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  ACI515_RS20260 (ACI515_20255) - 4472999..4473337 (-) 339 WP_010283921.1 DUF413 domain-containing protein -
  ACI515_RS20265 (ACI515_20260) hdfR 4473458..4474285 (+) 828 WP_039474331.1 HTH-type transcriptional regulator HdfR -
  ACI515_RS20270 (ACI515_20265) yjfF 4474307..4475269 (-) 963 WP_405078590.1 galactofuranose ABC transporter, permease protein YjfF -
  ACI515_RS20275 (ACI515_20270) ytfT 4475269..4476279 (-) 1011 WP_039474307.1 galactofuranose ABC transporter, ATP-binding protein YtfT -
  ACI515_RS20280 (ACI515_20275) ytfR 4476266..4477786 (-) 1521 WP_039547331.1 galactofuranose ABC transporter, ATP-binding protein YtfR -

Sequence

Protein


Download         Length: 508 a.a.        Molecular weight: 55517.18 Da        Isoelectric Point: 6.5677

>NTDB_id=1073379 ACI515_RS20255 WP_405078589.1 4471416..4472942(+) (comM) [Pectobacterium carotovorum strain M3]
MSLAVTYTRAMIGVQAPDVYIEVHISSGLPALTLVGLPETTVKEARDRVRSALINCGFTFPAKRITVNLAPADLPKEGGR
YDLPIALAILAASEQIDGEKLSRYEFLGELGLSGTLRGVNGAIPAALEAIKAGRQLILPDDNKREMTLIPQGEALMAGHL
LDVCAFLSGEEELLSCSDMTPVPPIEEDTLDLKDIIGQEQAKRALEIAAAGGHNLLLLGPPGTGKTMLASRLGNLMPSLS
DEEALESAAINSLINIDATMTRWRARPFRAPHHSSSMAALVGGGSLPKPGEISLAHNGVLFLDELPEFERRVLDSLREPL
ESGEIIISRTRAKVCYPARVQLVAAMNPSPSGHYQGVHNRLPAQQILRYLSKLSGPFLDRFDLSIEVPLLPPGVLSQQHY
QGESSATIRERVLIARQIQLKRANKINALLTSREIEKHCGLEMADATYLEEVMNKLGLSVRAWHRILKVARTIADLGERD
NIERKHLAEALSYRCMDRLLIQLHKSLE

Nucleotide


Download         Length: 1527 bp        

>NTDB_id=1073379 ACI515_RS20255 WP_405078589.1 4471416..4472942(+) (comM) [Pectobacterium carotovorum strain M3]
ATGTCATTGGCAGTTACCTATACTCGGGCAATGATTGGTGTACAAGCACCGGACGTTTATATCGAAGTTCACATCAGCAG
CGGGCTGCCGGCCCTAACGCTGGTGGGCTTACCTGAAACCACCGTCAAGGAAGCGCGCGATCGCGTGCGCAGCGCACTTA
TCAATTGTGGATTTACCTTTCCAGCAAAGCGCATCACGGTCAATCTCGCCCCCGCAGACCTGCCAAAAGAAGGGGGACGC
TACGATCTGCCGATTGCTCTGGCGATTCTGGCGGCCTCAGAACAAATCGATGGTGAAAAGCTAAGCCGCTACGAGTTTCT
TGGCGAACTCGGCCTGTCTGGCACCTTACGTGGCGTCAATGGCGCGATTCCCGCCGCATTAGAAGCCATAAAAGCAGGCC
GCCAGCTTATCCTGCCAGATGACAATAAACGGGAGATGACGCTGATACCGCAGGGTGAAGCGCTAATGGCCGGGCATTTG
TTGGACGTGTGTGCTTTTCTCAGTGGGGAAGAGGAATTGCTCAGTTGCTCCGACATGACGCCCGTTCCACCGATCGAGGA
AGATACCCTCGATCTGAAGGATATCATCGGTCAGGAGCAGGCTAAACGCGCGTTGGAAATCGCGGCGGCAGGAGGCCATA
ACTTGCTCTTACTGGGGCCACCGGGAACCGGGAAAACCATGCTGGCAAGCCGATTAGGCAATCTGATGCCGTCATTAAGC
GATGAAGAAGCGCTGGAAAGCGCCGCTATCAACAGTCTAATCAACATTGATGCCACCATGACGCGCTGGCGAGCCAGGCC
ATTCAGAGCACCTCACCATAGTTCCTCCATGGCTGCGCTCGTGGGCGGAGGGTCGCTGCCCAAACCCGGAGAGATCTCGC
TCGCCCACAACGGTGTACTGTTTTTGGACGAATTACCGGAGTTTGAGCGGCGCGTCCTGGACTCACTAAGAGAGCCTCTG
GAGTCCGGTGAAATTATCATTTCCCGCACGCGCGCCAAAGTATGCTACCCAGCACGCGTACAGCTCGTCGCCGCTATGAA
TCCCAGCCCCTCAGGACACTATCAGGGCGTTCATAACCGGTTGCCAGCACAACAAATACTGCGCTATCTCAGCAAACTTT
CCGGTCCCTTTTTGGATCGCTTTGACCTTTCAATCGAAGTCCCTTTGCTACCACCAGGGGTTTTATCTCAGCAACATTAT
CAAGGCGAAAGCAGCGCGACAATTCGCGAGCGCGTGCTGATCGCACGGCAGATACAGCTGAAGCGGGCAAATAAAATCAA
CGCACTGCTCACTTCACGTGAAATAGAAAAACACTGCGGATTGGAGATGGCTGATGCGACCTATTTGGAAGAGGTAATGA
ACAAACTTGGCTTATCCGTACGTGCATGGCACAGGATATTGAAAGTCGCGCGCACGATAGCTGACCTCGGCGAGCGGGAT
AATATTGAAAGGAAACACCTTGCCGAAGCGCTGAGCTATCGCTGTATGGATCGATTGCTCATCCAGCTCCACAAAAGCCT
TGAATAG

Domains

Predicted by InterproScan.

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(191-390)

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(402-494)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

63.834

99.606

0.636
  comM Vibrio cholerae strain A1552

64.016

99.016

0.634
  comM Glaesserella parasuis strain SC1401

63.261

100

0.634
  comM Vibrio campbellii strain DS40M4

62.648

99.606

0.624
  comM Legionella pneumophila str. Paris

50.701

98.228

0.498
  comM Legionella pneumophila strain ERS1305867

50.701

98.228

0.498
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

46.019

100

0.467

Multiple sequence alignment