Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACI3KK_RS19210 Genome accession   NZ_CP173421
Coordinates   3989850..3991553 (+) Length   567 a.a.
NCBI ID   WP_405118997.1    Uniprot ID   -
Organism   Pseudomonas leptonychotis strain TMP18     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3984850..3996553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3KK_RS19195 (ACI3KK_19205) nadC 3986370..3987218 (+) 849 WP_405118994.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACI3KK_RS19200 (ACI3KK_19210) - 3987404..3989104 (-) 1701 WP_405118995.1 Wzy polymerase domain-containing protein -
  ACI3KK_RS19205 (ACI3KK_19215) - 3989162..3989629 (-) 468 WP_405118996.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACI3KK_RS19210 (ACI3KK_19220) pilB 3989850..3991553 (+) 1704 WP_405118997.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACI3KK_RS19215 (ACI3KK_19225) pilC 3991557..3992774 (+) 1218 WP_405118998.1 type II secretion system F family protein Machinery gene
  ACI3KK_RS19220 (ACI3KK_19230) pilD 3992778..3993650 (+) 873 WP_405118999.1 prepilin peptidase Machinery gene
  ACI3KK_RS19225 (ACI3KK_19235) coaE 3993812..3994426 (+) 615 WP_405119000.1 dephospho-CoA kinase -
  ACI3KK_RS19230 (ACI3KK_19240) yacG 3994423..3994623 (+) 201 WP_405119001.1 DNA gyrase inhibitor YacG -
  ACI3KK_RS19235 (ACI3KK_19245) - 3994668..3994883 (-) 216 WP_405119002.1 hypothetical protein -
  ACI3KK_RS19240 (ACI3KK_19250) - 3994988..3995677 (-) 690 WP_405119003.1 energy-coupling factor ABC transporter permease -
  ACI3KK_RS19245 (ACI3KK_19255) - 3995822..3996451 (+) 630 WP_405119004.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62428.35 Da        Isoelectric Point: 5.3374

>NTDB_id=1072189 ACI3KK_RS19210 WP_405118997.1 3989850..3991553(+) (pilB) [Pseudomonas leptonychotis strain TMP18]
MNDSVSLTGLARQMVLAELLDEKTAQQAQSQAQRNKLSLVTYLVQNKLVKSRVLAELASEQFGVSFVDLNSIDKESQPKD
LVSEKLVRQHRVLPLWRRGNKLFIAVADPTNHQAVTDIQFSTGMNTEAILVEDDKLGEAIEKFFDSTTSGLDDLGDIDLD
GLDVEAVNDDSPDSGEGNAADDAPVVRFVNKMLLDAVKGGSSDLHFEPYEKSYRVRFRTDGILHEIARPPIQLSPRISAR
LKVMAGLDISERRKPQDGRIKMRISKSKSIDFRVSTCPTLWGEKIVMRILDSASAQMGIDALGYEEPQKELYMSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFTAALKSFLRQDPDIIMVGEIRD
LDTASIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCNSCKKEIQVPRETLLEEGFP
ESKIGAFKIYEPIGCENCNGGYKGRVGIYEVVKNTPALQRIIMEEGNSIDISIQMRKDGFNDLRTSALLKAMQGVTSLAE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1072189 ACI3KK_RS19210 WP_405118997.1 3989850..3991553(+) (pilB) [Pseudomonas leptonychotis strain TMP18]
ATGAACGACAGCGTGTCCCTTACCGGCCTTGCCCGGCAGATGGTTTTAGCAGAACTACTGGATGAAAAAACCGCCCAACA
AGCGCAAAGCCAGGCCCAGCGCAACAAGCTATCGCTGGTCACTTACTTGGTGCAGAACAAACTGGTTAAAAGTCGCGTGC
TGGCCGAGCTTGCTTCCGAGCAGTTCGGCGTGTCTTTCGTCGATTTGAATTCGATAGACAAAGAAAGCCAACCCAAAGAT
CTGGTGTCCGAAAAATTGGTTCGCCAACACCGCGTACTGCCGCTGTGGCGGCGTGGTAACAAGCTATTTATCGCTGTTGC
CGACCCCACCAATCACCAAGCAGTTACCGATATCCAGTTCAGCACAGGGATGAATACCGAAGCCATTCTGGTAGAAGACG
ACAAGCTTGGGGAAGCAATCGAGAAGTTCTTCGACTCAACCACTAGCGGCCTGGATGACCTGGGAGATATCGACCTAGAC
GGCCTAGATGTCGAGGCAGTCAATGACGACAGCCCGGATAGCGGCGAGGGCAACGCCGCCGACGATGCACCAGTTGTGCG
CTTCGTCAATAAAATGCTTTTAGATGCAGTTAAAGGTGGCTCATCCGACCTACACTTTGAGCCCTACGAGAAGTCCTACC
GAGTACGTTTCCGCACAGACGGTATTTTGCACGAAATAGCTCGGCCACCCATCCAGCTGTCACCACGAATTTCTGCTCGC
TTAAAGGTTATGGCTGGGCTGGATATTTCAGAACGACGTAAACCGCAGGATGGCCGCATCAAGATGCGCATATCCAAAAG
CAAATCCATCGACTTTCGCGTAAGCACCTGCCCAACCCTGTGGGGCGAAAAGATCGTGATGCGGATTCTAGACTCTGCCA
GCGCACAGATGGGCATCGACGCCCTAGGCTATGAAGAGCCGCAAAAAGAGCTATATATGTCCGCCCTGAAGCAGCCTCAA
GGCATGATTCTGGTGACAGGCCCTACTGGCTCCGGAAAAACAGTGTCCCTCTACACAGGCCTGAACATCCTCAACACCGT
CGACGTGAACATCTCCACAGCTGAAGACCCTGTGGAAATCAACTTGGAAGGCATCAATCAGGTCAACGTCAACCCCAAGC
AGGGCATGGATTTTACTGCAGCACTGAAGTCCTTCTTACGTCAAGACCCGGACATCATCATGGTCGGTGAGATTCGCGAC
TTGGATACCGCGTCAATCGCCATAAAGGCCGCACAAACCGGCCATATGGTGATGTCGACCCTACACACCAACAGCGCCGC
CGAGACCCTGACCCGCTTGCGCAATATGGGCGTCCCTTCGTTCAACATTGCCACCTCGGTCAACCTGATTATTGCTCAAC
GCTTGGCGCGCAAACTCTGTAATAGCTGCAAGAAGGAGATACAAGTCCCCCGAGAAACTCTTCTTGAGGAAGGATTTCCG
GAAAGCAAAATCGGAGCCTTCAAGATTTACGAACCGATCGGCTGCGAAAACTGCAACGGTGGCTACAAGGGCCGTGTCGG
AATTTATGAAGTGGTTAAAAACACGCCAGCCCTACAACGGATTATCATGGAAGAAGGCAACTCGATCGATATCTCCATCC
AGATGCGCAAAGACGGCTTCAATGACCTGCGAACCTCAGCCTTGCTGAAGGCCATGCAGGGCGTGACCAGCCTTGCAGAA
GTGAACCGTGTGACTAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.639

100

0.593
  pilB Acinetobacter baylyi ADP1

57.904

99.295

0.575
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.556
  pilB Vibrio cholerae strain A1552

50.53

99.824

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

51.571

95.414

0.492
  pilB Vibrio campbellii strain DS40M4

49.291

99.471

0.49
  pilF Neisseria gonorrhoeae MS11

48.759

99.471

0.485
  pilF Thermus thermophilus HB27

37.193

100

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.795

93.651

0.363

Multiple sequence alignment