Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACIO05_RS10180 Genome accession   NZ_CP173232
Coordinates   2152511..2152942 (-) Length   143 a.a.
NCBI ID   WP_035687024.1    Uniprot ID   A0A0F5ESJ8
Organism   Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2147511..2157942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIO05_RS10160 (ACIO05_10160) ispC 2148148..2149344 (-) 1197 WP_110479821.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
  ACIO05_RS10165 (ACIO05_10165) frr 2149526..2150083 (-) 558 WP_017807054.1 ribosome recycling factor -
  ACIO05_RS10170 (ACIO05_10170) pyrH 2150108..2150824 (-) 717 WP_035687020.1 UMP kinase -
  ACIO05_RS10175 (ACIO05_10175) pilB 2151074..2152498 (-) 1425 WP_035687022.1 GspE/PulE family protein Machinery gene
  ACIO05_RS10180 (ACIO05_10180) pilA 2152511..2152942 (-) 432 WP_035687024.1 pilin Machinery gene
  ACIO05_RS10185 (ACIO05_10185) ampD 2153082..2153636 (+) 555 WP_264245770.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACIO05_RS10190 (ACIO05_10190) - 2153735..2154154 (+) 420 WP_264256696.1 hypothetical protein -
  ACIO05_RS10195 (ACIO05_10195) rppH 2154726..2155319 (+) 594 WP_264245767.1 RNA pyrophosphohydrolase -
  ACIO05_RS10200 (ACIO05_10200) - 2155322..2156113 (+) 792 WP_264256695.1 sulfite exporter TauE/SafE family protein -
  ACIO05_RS10205 (ACIO05_10205) lgt 2156123..2156923 (+) 801 WP_264256694.1 prolipoprotein diacylglyceryl transferase -
  ACIO05_RS10210 (ACIO05_10210) - 2156935..2157786 (+) 852 WP_264256693.1 thymidylate synthase -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15205.70 Da        Isoelectric Point: 9.8115

>NTDB_id=1071026 ACIO05_RS10180 WP_035687024.1 2152511..2152942(-) (pilA) [Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-3]
MQNPTHLKIKKGFTLIELMIVIAIVAILATIAIPAYQNYTKKAAISELIQAASPYRSEVELCIYNTGATTKCNAGSNGIQ
KKLTNKGKVASIDVQKGTITVKGKDNLSDITYSLKASGDASKGVSWTVNCGKSVEFFPAGFCG

Nucleotide


Download         Length: 432 bp        

>NTDB_id=1071026 ACIO05_RS10180 WP_035687024.1 2152511..2152942(-) (pilA) [Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-3]
ATGCAAAACCCAACTCATTTAAAAATCAAAAAAGGCTTTACTTTGATTGAACTGATGATCGTGATTGCGATTGTGGCGAT
TTTAGCGACCATTGCGATTCCTGCTTATCAAAATTACACTAAGAAAGCGGCAATATCTGAGCTAATTCAAGCCGCAAGCC
CCTATCGTTCAGAAGTAGAATTATGTATTTATAATACGGGAGCTACCACGAAATGTAATGCGGGTTCAAATGGAATTCAG
AAAAAATTAACGAATAAAGGGAAAGTGGCTTCTATTGATGTACAAAAAGGCACGATTACGGTTAAAGGAAAAGATAATCT
TTCTGATATTACTTATTCATTAAAAGCCAGTGGAGATGCCTCAAAAGGTGTATCTTGGACGGTAAATTGTGGAAAATCTG
TAGAGTTTTTCCCAGCGGGATTTTGCGGATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F5ESJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

59.31

100

0.601
  pilA Haemophilus influenzae 86-028NP

57.931

100

0.587
  pilA Glaesserella parasuis strain SC1401

46.809

98.601

0.462
  pilA Vibrio campbellii strain DS40M4

34.615

100

0.378
  pilA Ralstonia pseudosolanacearum GMI1000

37.324

99.301

0.371

Multiple sequence alignment