Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACIO07_RS06320 Genome accession   NZ_CP173228
Coordinates   1222833..1224176 (+) Length   447 a.a.
NCBI ID   WP_410683430.1    Uniprot ID   -
Organism   Avibacterium paragallinarum strain npAP/GA-USA/20231220/S2-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1217833..1229176
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIO07_RS06290 (ACIO07_06295) lgt 1218408..1219208 (-) 801 WP_017807046.1 prolipoprotein diacylglyceryl transferase -
  ACIO07_RS06295 (ACIO07_06300) - 1219218..1220009 (-) 792 WP_410680657.1 sulfite exporter TauE/SafE family protein -
  ACIO07_RS06300 (ACIO07_06305) rppH 1220012..1220605 (-) 594 WP_410683428.1 RNA pyrophosphohydrolase -
  ACIO07_RS06305 (ACIO07_06310) - 1221177..1221596 (-) 420 WP_264256696.1 hypothetical protein -
  ACIO07_RS06310 (ACIO07_06315) ampD 1221695..1222249 (-) 555 WP_410683429.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACIO07_RS06315 (ACIO07_06320) pilA 1222389..1222820 (+) 432 WP_035687024.1 pilin Machinery gene
  ACIO07_RS06320 (ACIO07_06325) pilB 1222833..1224176 (+) 1344 WP_410683430.1 GspE/PulE family protein Machinery gene
  ACIO07_RS06325 (ACIO07_06330) - 1224118..1225065 (-) 948 WP_200896145.1 IS110 family transposase -
  ACIO07_RS06330 (ACIO07_06335) pyrH 1225655..1226371 (+) 717 WP_410683431.1 UMP kinase -
  ACIO07_RS06335 (ACIO07_06340) frr 1226396..1226953 (+) 558 WP_017807054.1 ribosome recycling factor -
  ACIO07_RS06340 (ACIO07_06345) ispC 1227134..1228330 (+) 1197 WP_110479821.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -

Sequence

Protein


Download         Length: 447 a.a.        Molecular weight: 50861.53 Da        Isoelectric Point: 6.5017

>NTDB_id=1070916 ACIO07_RS06320 WP_410683430.1 1222833..1224176(+) (pilB) [Avibacterium paragallinarum strain npAP/GA-USA/20231220/S2-3]
MAEYSLDEDDIVYAVDGKCFEITPHLWQQNKQQKDILIRYLAVPLQEDEHTLWLGIDDLTNLSACESFSFLTGKIVEPIL
LSEQDLKSLLQKLSPTLVEEPALLDFYQSSISPYSENQPEKLDEPIIQILNKVFESAMQLNASDIHLEPQNEQLQIRFRI
DGVLQHQPPISKQMSQRLISRLKLLARLDLSETRLPQDGRFHFKTTFSDILDFRISTLPTYYGEKAVLRLQKNKPVTLDF
SELGMTAIQRKTLQNALSQPQGLILVTGPTGSGKSITLYTALEWLNQSEKHILTAEDPIEIELPGIIQTQINPQIGLDFN
RLLRTFLRQDPDIIMLGEIRDAESAAIALRAAQTGHLVLSTLHTNNAHSAIARLLQLGIQQYEIESCLLLVIAQRLVRKK
CLHKTKNCECYQGYSNCIPLKSKNTNSLIKVRERPIASHRGQSHLKW

Nucleotide


Download         Length: 1344 bp        

>NTDB_id=1070916 ACIO07_RS06320 WP_410683430.1 1222833..1224176(+) (pilB) [Avibacterium paragallinarum strain npAP/GA-USA/20231220/S2-3]
ATGGCTGAGTATTCTTTAGATGAGGACGACATTGTATATGCTGTTGATGGAAAATGCTTTGAAATAACACCGCACTTATG
GCAACAAAATAAGCAGCAAAAAGATATTTTAATACGTTATTTAGCAGTACCTTTACAGGAGGATGAACATACACTTTGGC
TTGGTATTGATGACTTAACAAATCTATCGGCTTGTGAAAGTTTTTCATTTCTAACAGGAAAAATAGTTGAGCCGATTTTG
TTATCAGAACAAGATTTAAAATCGCTATTACAGAAGTTATCTCCCACATTAGTAGAAGAGCCGGCATTACTTGATTTTTA
TCAATCGTCTATTTCACCTTATAGCGAAAATCAACCAGAAAAACTTGATGAACCAATTATTCAAATACTAAATAAAGTTT
TTGAATCGGCAATGCAATTAAATGCCTCAGATATTCATTTAGAGCCACAGAATGAGCAATTACAGATCCGCTTCCGTATT
GACGGGGTATTGCAACATCAGCCTCCTATTTCTAAACAAATGAGCCAACGTTTGATTTCTCGTTTAAAACTTCTAGCTCG
TCTTGATCTTAGCGAAACTCGCTTACCACAAGATGGAAGATTTCATTTCAAAACGACATTTTCCGATATATTAGACTTCC
GTATATCGACATTACCTACATATTATGGAGAAAAAGCGGTACTACGTTTACAAAAAAATAAACCTGTTACCTTGGATTTT
TCTGAGCTGGGGATGACAGCGATACAACGAAAAACATTACAAAATGCTTTATCTCAACCTCAAGGACTCATTTTAGTAAC
GGGGCCAACCGGGAGTGGAAAAAGTATTACGCTCTATACAGCATTAGAATGGCTTAATCAATCGGAAAAACATATTCTAA
CAGCAGAAGATCCGATTGAAATAGAACTTCCAGGGATTATCCAAACGCAAATTAACCCACAAATTGGTTTAGATTTTAAC
CGTTTGTTGCGTACATTTTTACGTCAGGATCCAGACATTATTATGCTTGGTGAGATCCGTGATGCAGAAAGTGCAGCGAT
TGCGTTACGTGCAGCACAAACTGGACATTTAGTCCTTTCAACATTACATACAAATAATGCACATTCAGCTATTGCTCGCT
TGTTACAGCTTGGTATTCAACAATATGAAATCGAAAGTTGTTTATTATTAGTGATTGCCCAGCGTTTAGTCCGTAAAAAA
TGTCTACACAAAACGAAAAATTGCGAATGTTATCAAGGTTATAGCAACTGTATTCCGCTAAAAAGCAAAAATACAAATTC
GTTAATTAAAGTGCGTGAGCGTCCCATTGCGAGTCATCGCGGACAATCGCATTTAAAATGGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-95)

Type II/IV secretion system protein (T2SSE)

(132-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

66.588

94.407

0.629
  pilB Haemophilus influenzae 86-028NP

65.877

94.407

0.622
  pilB Glaesserella parasuis strain SC1401

60.88

91.499

0.557
  pilB Legionella pneumophila strain ERS1305867

45.503

84.564

0.385
  pilB Vibrio campbellii strain DS40M4

44.094

85.235

0.376
  pilB Vibrio cholerae strain A1552

43.57

85.235

0.371
  pilB Acinetobacter baylyi ADP1

42.455

87.472

0.371
  pilB Vibrio parahaemolyticus RIMD 2210633

43.307

85.235

0.369

Multiple sequence alignment