Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAM54_RS20775 Genome accession   NZ_AP028669
Coordinates   4383517..4385250 (+) Length   577 a.a.
NCBI ID   WP_145740448.1    Uniprot ID   -
Organism   Variovorax sp. V93     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4378517..4390250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM54_RS20740 (GmRootV93_41230) - 4378584..4379114 (+) 531 WP_369648806.1 CNP1-like family protein -
  ACAM54_RS20745 (GmRootV93_41240) proB 4379179..4380318 (-) 1140 WP_369648807.1 glutamate 5-kinase -
  ACAM54_RS20750 (GmRootV93_41250) cgtA 4380356..4381444 (-) 1089 WP_369648808.1 Obg family GTPase CgtA -
  ACAM54_RS20755 (GmRootV93_41260) rpmA 4381515..4381772 (-) 258 WP_015866991.1 50S ribosomal protein L27 -
  ACAM54_RS20760 (GmRootV93_41270) rplU 4381785..4382096 (-) 312 WP_015866992.1 50S ribosomal protein L21 -
  ACAM54_RS20765 (GmRootV93_41280) - 4382285..4383277 (+) 993 WP_145740450.1 polyprenyl synthetase family protein -
  ACAM54_RS20775 (GmRootV93_41290) pilB 4383517..4385250 (+) 1734 WP_145740448.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAM54_RS20780 (GmRootV93_41300) pilC 4385280..4386509 (+) 1230 WP_369648809.1 type II secretion system F family protein Machinery gene
  ACAM54_RS20785 (GmRootV93_41310) - 4386509..4387474 (+) 966 WP_369648810.1 A24 family peptidase -
  ACAM54_RS20790 (GmRootV93_41320) coaE 4387485..4388090 (+) 606 WP_192322490.1 dephospho-CoA kinase -
  ACAM54_RS20795 (GmRootV93_41330) - 4388168..4389661 (+) 1494 WP_369648811.1 MBOAT family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62740.95 Da        Isoelectric Point: 6.2073

>NTDB_id=106826 ACAM54_RS20775 WP_145740448.1 4383517..4385250(+) (pilB) [Variovorax sp. V93]
MAAAELPVKENTQIALPGLARALVSAGKLPAKTAEDIYQKSLNGRTSFIAELTGSGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCHAYRIVVLSKRNNRLIVATADPSDQQAVEKIKFASQMGVDWVIAEYDKLSRMIEAAAVTAAESI
NSIVGAEFEFDDVTADTSGDANEQAIAEVEDAPVVRFLHKMLLDGVSMRASDIHFEPYEHNYRVRFRIDGELREIASPPT
LIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYDADEKER
LLHAISRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCHMCRAPADVPR
QALLDAGFKEEQLDGTWKPYRPVGCAACSNGYKGRVGIYQVMPISEAIQAIILRDGSAQDIARQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=106826 ACAM54_RS20775 WP_145740448.1 4383517..4385250(+) (pilB) [Variovorax sp. V93]
ATGGCTGCTGCCGAACTTCCTGTTAAAGAAAACACGCAAATCGCCCTCCCGGGCCTTGCGCGTGCCTTGGTCTCTGCTGG
CAAGCTTCCGGCGAAGACCGCGGAAGATATCTACCAGAAGTCGCTGAACGGCCGCACCAGCTTCATTGCCGAGCTGACCG
GCAGCGGCGCCGTCTCGGCCGCCGACCTCGCCCACACGCTGTCCAGCGCCTTCGGTGCGCCGCTGCTGGACCTGGACGCC
ATCGATCACCAGCGCCTGCCCAAGGACCTGCTCGACCCGAAGCTGTGCCACGCCTACCGGATCGTGGTTCTCAGCAAGCG
CAACAACCGCCTCATCGTTGCAACGGCCGACCCTTCGGACCAGCAGGCGGTGGAAAAGATCAAGTTCGCCTCCCAGATGG
GTGTGGACTGGGTCATTGCCGAGTACGACAAGCTGTCGCGCATGATCGAGGCCGCAGCAGTCACCGCAGCGGAATCCATC
AACAGCATCGTCGGTGCCGAGTTCGAGTTCGACGACGTCACCGCGGACACTTCGGGCGATGCCAACGAACAGGCCATCGC
CGAGGTCGAGGACGCGCCAGTCGTGCGCTTCCTGCACAAGATGCTGCTCGACGGCGTCAGCATGCGGGCCTCGGACATCC
ACTTCGAGCCCTACGAGCACAACTACCGGGTGCGCTTTCGCATCGACGGCGAGCTGCGCGAGATCGCGAGCCCGCCCACG
CTCATCAAGGACAAGCTGGCCTCGCGGATCAAGGTCATTTCACGGCTCGACATTTCCGAGAAGCGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGGCCCGACCGCGTCATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAGA
TCGTGATCCGTATCCTCGACCCGAGCAGCGCGCGCCTGGGCATCGACGCCCTGGGCTACGACGCCGACGAAAAGGAGCGC
CTGCTGCATGCGATCAGCCGCCCCTACGGCATGGTGCTGGTCACGGGCCCCACCGGTTCGGGCAAGACGGTGTCGCTCTA
CACCTGCCTGAACCTGCTGAACCAGCCGGGCGTCAACATCGCGACGGCGGAAGACCCCTCTGAAATCAACCTGCCGGGCG
TCAACCAGGTGAACGTCAACGAGCGCGCCGGACTCACCTTCGCGGCGGCGCTGCGCGCCTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCGCAAACGGGCCACCTGGTGCT
CTCGACGCTGCACACCAACGACGCGCCGACCACGCTCACGCGCATGCGCAACATGGGCATTGCGCCGTTCAACATCGCCT
CCAGCGTGATCCTGATCACGGCGCAGCGGCTCGCGCGGCGCCTGTGCCACATGTGCCGCGCGCCGGCCGACGTGCCGCGC
CAGGCGCTGCTCGACGCGGGCTTCAAGGAAGAACAGCTTGACGGTACCTGGAAGCCCTACCGGCCGGTCGGCTGCGCGGC
GTGCAGCAATGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATCTCGGAGGCGATCCAGGCCATCATCCTGC
GCGACGGCAGCGCACAGGACATCGCGCGACAGTCCGAGGCCGAGGGCGTGCGCTCGCTGCGGCAGTCTGGCCTGAGAAAA
GTCATGCAAGGGCTCACCTCACTCGAAGAAGTGGTGGCGGTCACGAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-152)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.697

98.44

0.529
  pilB Acinetobacter baylyi ADP1

53.476

97.227

0.52
  pilF Neisseria gonorrhoeae MS11

52.398

97.574

0.511
  pilB Legionella pneumophila strain ERS1305867

49.027

97.92

0.48
  pilB Vibrio campbellii strain DS40M4

45.664

97.92

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.633

97.227

0.444
  pilB Vibrio cholerae strain A1552

48.077

90.121

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.377

91.854

0.371
  pilF Thermus thermophilus HB27

36.888

99.133

0.366

Multiple sequence alignment