Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACF8YQ_RS12045 Genome accession   NZ_CP172297
Coordinates   2603363..2604238 (+) Length   291 a.a.
NCBI ID   WP_396621539.1    Uniprot ID   -
Organism   Marinobacter sp. W-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2598363..2609238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF8YQ_RS12030 pilE 2599504..2599974 (-) 471 WP_396621536.1 pilin Machinery gene
  ACF8YQ_RS12035 pilB 2600343..2602061 (+) 1719 WP_396621537.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACF8YQ_RS12040 pilC 2602064..2603281 (+) 1218 WP_396621538.1 type II secretion system F family protein Machinery gene
  ACF8YQ_RS12045 pilD 2603363..2604238 (+) 876 WP_396621539.1 prepilin peptidase Machinery gene
  ACF8YQ_RS12050 coaE 2604239..2604856 (+) 618 WP_396621540.1 dephospho-CoA kinase -
  ACF8YQ_RS12055 - 2604945..2605934 (+) 990 WP_396621541.1 hypothetical protein -
  ACF8YQ_RS12060 tsaA 2605943..2606692 (+) 750 WP_011786131.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  ACF8YQ_RS12065 - 2606737..2609091 (+) 2355 WP_121207110.1 response regulator -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32185.45 Da        Isoelectric Point: 7.5453

>NTDB_id=1066090 ACF8YQ_RS12045 WP_396621539.1 2603363..2604238(+) (pilD) [Marinobacter sp. W-8]
MLPLETILSTPWLLYLAVIFISLCVGSFLNVVILRLPKMMKQEWRCQCEEFLEVPESGRKPDERITLSKPASTCPSCGHK
IRAWENIPVISWLMLGGKCSSCKTRISPRYPIIEALTAVFSVVTVYLLGPSESALWALLMVWALVALTMIDFDTQLLPDG
ITLPLMWLGLVLNYFGVLTDFGSAFWGAVAGYLSLWSVYWLFKLATGKEGMGHGDFKLLAALGAWLGWQLLPAVILLSSL
VGAIVGISLMVFRKHGREVPIPFGPYLAAAGLLSLWFGPEIQAIWFGYLGV

Nucleotide


Download         Length: 876 bp        

>NTDB_id=1066090 ACF8YQ_RS12045 WP_396621539.1 2603363..2604238(+) (pilD) [Marinobacter sp. W-8]
ATGCTCCCCCTAGAAACAATCCTGTCCACCCCCTGGCTTCTCTACCTGGCGGTCATTTTCATCTCTCTCTGCGTCGGCAG
CTTTCTTAACGTCGTCATCCTGCGCCTGCCCAAAATGATGAAGCAGGAATGGCGCTGCCAGTGCGAGGAGTTTCTGGAAG
TACCGGAGAGCGGCCGCAAGCCGGATGAGCGGATAACTCTTTCAAAACCGGCATCCACCTGCCCCTCCTGCGGCCACAAA
ATTCGCGCCTGGGAAAACATTCCGGTCATCAGCTGGCTAATGCTTGGCGGCAAATGCTCTTCATGCAAAACACGTATTTC
GCCGCGTTACCCCATCATTGAAGCACTGACTGCGGTTTTTTCCGTAGTAACGGTTTACCTGTTGGGTCCATCTGAGTCCG
CACTCTGGGCGTTGCTGATGGTCTGGGCGCTGGTGGCGCTGACCATGATCGATTTCGACACACAACTCCTGCCAGACGGC
ATCACCCTGCCCCTGATGTGGCTTGGACTGGTGCTTAACTACTTTGGTGTGCTGACGGATTTTGGCAGCGCTTTCTGGGG
CGCTGTTGCCGGTTATCTGTCACTCTGGAGTGTGTACTGGTTGTTCAAGCTTGCTACTGGCAAGGAGGGCATGGGGCACG
GGGATTTCAAGCTGTTGGCGGCGTTGGGCGCCTGGCTGGGTTGGCAACTGCTGCCTGCGGTGATTCTGCTCTCCTCTCTG
GTGGGCGCGATTGTGGGCATCAGTCTGATGGTATTCCGCAAACACGGGCGTGAAGTGCCGATTCCTTTCGGCCCTTACCT
GGCCGCTGCAGGCTTACTCTCGTTGTGGTTTGGGCCTGAAATCCAGGCCATATGGTTCGGGTACCTGGGAGTCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(139-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

55.474

94.158

0.522
  pilD Acinetobacter nosocomialis M2

54.745

94.158

0.515
  pilD Vibrio campbellii strain DS40M4

52.632

97.938

0.515
  pilD Vibrio cholerae strain A1552

52.857

96.22

0.509
  pilD Neisseria gonorrhoeae MS11

48.473

90.034

0.436

Multiple sequence alignment