Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACF8YQ_RS03085 Genome accession   NZ_CP172297
Coordinates   676643..677707 (+) Length   354 a.a.
NCBI ID   WP_036149326.1    Uniprot ID   -
Organism   Marinobacter sp. W-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 671643..682707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF8YQ_RS03070 rpmE 672079..672300 (+) 222 WP_014420566.1 50S ribosomal protein L31 -
  ACF8YQ_RS03075 - 672515..673780 (+) 1266 WP_396622557.1 malic enzyme-like NAD(P)-binding protein -
  ACF8YQ_RS03080 - 673865..676405 (-) 2541 WP_396622558.1 penicillin-binding protein 1A -
  ACF8YQ_RS03085 pilM 676643..677707 (+) 1065 WP_036149326.1 pilus assembly protein PilM Machinery gene
  ACF8YQ_RS03090 - 677707..678270 (+) 564 WP_011784346.1 PilN domain-containing protein -
  ACF8YQ_RS03095 - 678270..678881 (+) 612 WP_396622559.1 type 4a pilus biogenesis protein PilO -
  ACF8YQ_RS03100 - 678884..679426 (+) 543 WP_153741467.1 pilus assembly protein PilP -
  ACF8YQ_RS03105 pilQ 679441..681552 (+) 2112 WP_224851273.1 type IV pilus secretin PilQ family protein Machinery gene
  ACF8YQ_RS03110 aroK 681688..682278 (+) 591 WP_396622560.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38021.04 Da        Isoelectric Point: 4.1824

>NTDB_id=1066075 ACF8YQ_RS03085 WP_036149326.1 676643..677707(+) (pilM) [Marinobacter sp. W-8]
MFGLPGKKSSAVLGVDVSSSSVKLLELSKHGDRYKVESYAVEPLPANAVVEKNITDVEAVGEVLKRVASKSRTSVKQVAV
AVSGSAVITKVIQMDGGLNEFEMEDQIALEADQYIPYPLDEVAIDFEVQGPSESNPDQVDVLLAACRKENVDIREDALEI
AGLTTRIVDVEAYALERAYSLIEPQLDSQGEELVVAIVDIGATMTTLSVLAEGKTVYTREQIFGGKQLTEEIQRRYGLSL
EEAGLAKKQGGLPDDYNEEVLTPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIQGLTEMVEEKTGTPTLVANP
FANMAVGSRVNASALSNDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1066075 ACF8YQ_RS03085 WP_036149326.1 676643..677707(+) (pilM) [Marinobacter sp. W-8]
GTGTTCGGATTACCAGGAAAAAAATCCAGTGCCGTCCTTGGAGTGGATGTAAGCTCCAGTTCGGTCAAGCTGCTAGAGCT
GTCAAAACATGGCGATCGATACAAGGTCGAGAGCTATGCGGTAGAGCCGTTGCCGGCCAATGCAGTTGTTGAAAAAAACA
TCACAGACGTCGAAGCGGTTGGGGAAGTGCTCAAGCGTGTCGCGTCCAAGTCACGTACCAGTGTGAAACAGGTGGCTGTT
GCTGTTTCCGGCTCGGCGGTGATCACCAAGGTTATCCAGATGGATGGTGGTCTCAACGAGTTTGAAATGGAAGACCAGAT
TGCGCTGGAAGCCGATCAGTACATTCCTTACCCCCTCGATGAAGTGGCGATCGATTTCGAGGTCCAGGGCCCGTCCGAAT
CCAACCCGGATCAGGTGGATGTGCTGCTGGCGGCCTGCCGTAAGGAAAACGTCGACATCCGCGAAGACGCCCTGGAAATT
GCAGGGCTGACAACCAGGATTGTCGACGTGGAAGCCTATGCTCTGGAGCGGGCTTACAGCCTGATTGAACCTCAGCTCGA
CTCCCAGGGTGAAGAGTTGGTGGTTGCCATTGTTGATATTGGCGCGACCATGACAACTCTGAGTGTGCTGGCAGAGGGTA
AGACGGTCTATACCCGCGAGCAGATTTTTGGCGGAAAACAGCTGACGGAAGAAATTCAGCGTCGTTACGGGTTGTCGCTC
GAAGAAGCTGGTCTGGCCAAGAAGCAGGGTGGTTTGCCAGACGACTACAACGAGGAAGTCCTGACCCCGTTCCGGGAAGC
GGTTGTTCAGCAAGTGGCGCGGGCGCTGCAGTTTTTCTTCGGCGCGAGTCAGTACAACGCGGTGGATTACGTGGTGCTTG
CCGGAGGAACAGCGTCGATTCAGGGGCTCACGGAAATGGTGGAAGAGAAAACTGGAACGCCAACCCTGGTGGCCAATCCG
TTTGCCAATATGGCGGTGGGCTCCCGGGTTAATGCCTCTGCGCTAAGCAATGATGCCCCCTCGTTGATGATTGCCTGCGG
TCTGGCAATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.39

100

0.534
  comM Acinetobacter nosocomialis M2

53.39

100

0.534
  comM Acinetobacter baylyi ADP1

51.412

100

0.514
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.449

Multiple sequence alignment