Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACG1WH_RS16440 Genome accession   NZ_CP172238
Coordinates   3572526..3574259 (-) Length   577 a.a.
NCBI ID   WP_049403029.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain SM1021     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3567526..3579259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG1WH_RS16425 sucC 3567841..3569010 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  ACG1WH_RS16430 - 3569368..3570981 (+) 1614 WP_005414304.1 PAS domain-containing sensor histidine kinase -
  ACG1WH_RS16435 pilR 3571042..3572415 (+) 1374 WP_111108606.1 sigma-54 dependent transcriptional regulator Regulator
  ACG1WH_RS16440 pilB 3572526..3574259 (-) 1734 WP_049403029.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACG1WH_RS16445 - 3574482..3574931 (-) 450 WP_275043866.1 pilin -
  ACG1WH_RS16450 - 3575042..3575464 (-) 423 WP_049403031.1 pilin -
  ACG1WH_RS16455 pilC 3575817..3577076 (+) 1260 WP_049403032.1 type II secretion system F family protein Machinery gene
  ACG1WH_RS16460 - 3577084..3577947 (+) 864 WP_005410800.1 A24 family peptidase -
  ACG1WH_RS16465 coaE 3577959..3578570 (+) 612 WP_049403033.1 dephospho-CoA kinase -
  ACG1WH_RS16470 - 3578629..3579015 (-) 387 WP_005410802.1 hypothetical protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62390.35 Da        Isoelectric Point: 6.0438

>NTDB_id=1065847 ACG1WH_RS16440 WP_049403029.1 3572526..3574259(-) (pilB) [Stenotrophomonas maltophilia strain SM1021]
MNAVTTANLVGITGLARRLVQDGALDEATARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGATGGESGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRRSSLPE
HALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1065847 ACG1WH_RS16440 WP_049403029.1 3572526..3574259(-) (pilB) [Stenotrophomonas maltophilia strain SM1021]
ATGAACGCTGTCACCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTGCAGGACGGGGCCCTGGACGA
GGCCACCGCCCGCGACGCCATGGCCAAGGCCGCCGCCGCGCGCCAGCCACTGCCGACGTGGTTCGCGCAGAACAAGCTGG
TCAATGCCTCGCAGCTGGCCGCCGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACCTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTCTTCAAGCGCGGTGGCAAGCT
GTTCGTCGGCACCAGCAATCCGACCCACTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTCGAGCCGATCCTGG
TCGACGAGGACCAGATCCGGCGCACGCTGGAACAGTGGCACGCCAGCCATGACACCCTCGGCGATGCCCTGGGTGGTGAT
GACGATGGCATGGGCAACCTGGACGTGGCGGCCGGCGACGAGGATCTCGGCGCCACCGGCGGCGAGTCCGGCATCGATGC
CAAGGGTGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCCTCGGACATCC
ACTTCGAGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTCGCGCGCGCACCGGTG
AAGTTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCACAGCTGGATATCGCCGAGAAGCGCGTCCCGCAGGACGG
CCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGG
TGGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTG
TTCCTGGATGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTA
TACCGCGCTGGGCATCCTCAATGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCG
TCAACCAGGTACAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGACAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCGGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCGTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGCCTGTGCGGCAACTGCAAGCGCCGGTCCAGCCTGCCCGAA
CATGCACTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCGAGCTGTACGAGGCCGTGGGCTGCGATGA
ATGCACCGAGGGCTACAAGGGCCGGACCGGCGTCTACCAGGTCATGCCGATGACCGATGAGATCGCCACCATCGTGCTGG
CCGGTGGCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGCGTGAATGACCTGCGCCAGTCGGCCTTGAAGAAG
GCGGCGGCGGGCATCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.077

100

0.555
  pilB Acinetobacter baylyi ADP1

54.53

100

0.553
  pilB Legionella pneumophila strain ERS1305867

52.105

98.787

0.515
  pilF Neisseria gonorrhoeae MS11

48.772

98.787

0.482
  pilB Vibrio cholerae strain A1552

48.936

97.747

0.478
  pilB Vibrio campbellii strain DS40M4

45.518

98.614

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

45.583

98.094

0.447

Multiple sequence alignment