Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACH19Q_RS02425 Genome accession   NZ_CP172236
Coordinates   526158..527885 (-) Length   575 a.a.
NCBI ID   WP_071507140.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain UMN24-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 521158..532885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH19Q_RS02395 (ACH19Q_02395) - 521781..522242 (+) 462 WP_011002737.1 NUDIX domain-containing protein -
  ACH19Q_RS02400 (ACH19Q_02400) yacG 522235..522450 (-) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  ACH19Q_RS02405 (ACH19Q_02405) zapD 522461..523219 (-) 759 WP_016723794.1 cell division protein ZapD -
  ACH19Q_RS02410 (ACH19Q_02410) coaE 523337..523951 (-) 615 WP_011002734.1 dephospho-CoA kinase -
  ACH19Q_RS02415 (ACH19Q_02415) - 523964..524845 (-) 882 WP_029240316.1 prepilin peptidase -
  ACH19Q_RS02420 (ACH19Q_02420) pilC 524848..526113 (-) 1266 WP_201016383.1 type II secretion system F family protein Machinery gene
  ACH19Q_RS02425 (ACH19Q_02425) pilB 526158..527885 (-) 1728 WP_071507140.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACH19Q_RS02430 (ACH19Q_02430) - 528133..529410 (-) 1278 WP_165591155.1 HlyC/CorC family transporter -
  ACH19Q_RS02440 (ACH19Q_02440) ispB 529698..530666 (-) 969 WP_111374599.1 octaprenyl diphosphate synthase -
  ACH19Q_RS02445 (ACH19Q_02445) rplU 530945..531256 (+) 312 WP_011002728.1 50S ribosomal protein L21 -
  ACH19Q_RS02450 (ACH19Q_02450) rpmA 531294..531554 (+) 261 WP_003271630.1 50S ribosomal protein L27 -
  ACH19Q_RS02455 (ACH19Q_02455) obgE 531731..532831 (+) 1101 WP_011002727.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63826.67 Da        Isoelectric Point: 7.1546

>NTDB_id=1065815 ACH19Q_RS02425 WP_071507140.1 526158..527885(-) (pilB) [Ralstonia pseudosolanacearum strain UMN24-1]
MTLGLSLAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEVCNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1065815 ACH19Q_RS02425 WP_071507140.1 526158..527885(-) (pilB) [Ralstonia pseudosolanacearum strain UMN24-1]
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCACCCAGCTAGAACAAAGCGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCGCAATACAAGCTCGACACCGTGCCGCCTGTGCTGTCGGCCAACAGGCATTTT
CAGCAGTTGCGCCTGTTGCCGCTGGGCCGGCGTGACAACCGCATCATCGTGGCCACGTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCTCCAACGAGGCACCGGCTGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCACGCAGATCGAATACGACCCGGGT
GCGGCCGCGGCGGTCCGCCGCACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATATCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGATGGTCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAGGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCAGGGCTGACCTTCGCCGCGGCGCTGAAGTCCTTTCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCGCCATTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGTCCACTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAGGAAGCAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGTATGCAACGG
CAGCGGCTACAAGGGTCGAGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCCGGCCTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-131)

Type II/IV secretion system protein (T2SSE)

(184-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.06

98.435

0.503
  pilF Neisseria gonorrhoeae MS11

52.93

94.957

0.503
  pilB Acinetobacter baylyi ADP1

49.554

97.565

0.483
  pilB Legionella pneumophila strain ERS1305867

47.163

98.087

0.463
  pilB Vibrio cholerae strain A1552

44.821

97.391

0.437
  pilB Vibrio parahaemolyticus RIMD 2210633

45.203

94.261

0.426
  pilB Vibrio campbellii strain DS40M4

44.526

95.304

0.424
  pilF Thermus thermophilus HB27

38.434

97.739

0.376

Multiple sequence alignment