Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PN837_RS16890 Genome accession   NZ_CP171822
Coordinates   3995305..3997017 (+) Length   570 a.a.
NCBI ID   WP_395374933.1    Uniprot ID   -
Organism   Marinicella sp. W31     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3990305..4002017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PN837_RS16870 (PN837_016870) - 3990712..3992145 (-) 1434 WP_395374929.1 DUF4139 domain-containing protein -
  PN837_RS16875 (PN837_016875) - 3992235..3992672 (-) 438 WP_395374930.1 DUF4426 domain-containing protein -
  PN837_RS16880 (PN837_016880) sucD 3992835..3993707 (-) 873 WP_395374931.1 succinate--CoA ligase subunit alpha -
  PN837_RS16885 (PN837_016885) sucC 3993718..3994878 (-) 1161 WP_395374932.1 ADP-forming succinate--CoA ligase subunit beta -
  PN837_RS16890 (PN837_016890) pilB 3995305..3997017 (+) 1713 WP_395374933.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PN837_RS16895 (PN837_016895) pilC 3997020..3998246 (+) 1227 WP_395374934.1 type II secretion system F family protein Machinery gene
  PN837_RS16900 (PN837_016900) - 3998288..3999109 (+) 822 WP_395374935.1 prepilin peptidase -
  PN837_RS16905 (PN837_016905) coaE 3999140..3999748 (+) 609 WP_395374936.1 dephospho-CoA kinase -
  PN837_RS16915 (PN837_016915) - 4000114..4001172 (+) 1059 WP_395374937.1 pilus assembly protein PilM -
  PN837_RS16920 (PN837_016920) - 4001172..4001768 (+) 597 WP_395374938.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62735.00 Da        Isoelectric Point: 5.3064

>NTDB_id=1064431 PN837_RS16890 WP_395374933.1 3995305..3997017(+) (pilB) [Marinicella sp. W31]
MSQPHVKRVVNGTLNRLIQSSHLTLDVAIDATEKSKSQKIPLFTYLIENDLVNPNDLAQAASIEYGVPLLDITAYNLTMV
ALNLVEERLIDKHQVLPLFKRGTKIYVAVSDPTNARALDEIRFHTNLSVEAILVQHDDLASAIEEALKQNSSMMDDLGDD
GLEDLNFDEAIEDDTQSAGDSDDLADTPVVKFVNKVLLDAIKRGASDIHFEPYERTYRVRYRIDGVLTTMAKPPKNMTLK
ISSRLKVMANLDIAEKRVPQDGRVKLNLSKTNAIDFRMSTCPTLFGEKIVMRVLDASAAKMGVDKLGYEEEQKRLFLDAI
QKPYGMVLVTGPTGSGKTVSLYTALNILNIEGTNISTVEDPVEIRVHGINQVQQNTKQGMTFAAALRSFLRQDPDVIMVG
EIRDLETAEIAIKAAQTGHMVLSTLHTNNAPETISRLMNMGVAPFNITSAVALVVAQRLARRLHDCKAATELPKEALLEA
GMSEEDLVDAQIFDAVGCRKCNQGYKARVGIYEVMPLSTNIKKIILNSGNALDIADQALKDGIADLRQSALLKVRNGITS
LVEANRVTVD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1064431 PN837_RS16890 WP_395374933.1 3995305..3997017(+) (pilB) [Marinicella sp. W31]
ATGTCGCAACCGCATGTGAAAAGAGTGGTCAATGGGACACTGAATCGATTGATTCAGAGTTCACATTTGACGCTGGATGT
GGCAATAGACGCCACAGAAAAGTCAAAATCACAAAAAATACCTCTTTTTACCTACCTGATTGAAAACGACCTTGTTAATC
CAAATGACCTAGCACAAGCTGCATCCATAGAGTATGGTGTTCCGCTACTGGACATCACGGCTTATAACCTCACCATGGTG
GCATTAAACTTGGTTGAGGAAAGGCTTATCGATAAACATCAGGTTTTGCCGTTGTTTAAGCGTGGAACCAAAATATATGT
AGCTGTTTCTGATCCTACCAATGCCAGAGCTTTGGATGAAATTCGTTTTCATACCAACCTGTCAGTAGAAGCGATTTTGG
TACAACATGATGATTTGGCATCTGCTATAGAAGAAGCATTGAAGCAAAATTCTTCTATGATGGATGATCTTGGGGATGAT
GGTCTGGAAGACTTGAACTTCGACGAAGCCATCGAAGATGACACTCAATCAGCAGGTGACTCTGATGACCTTGCCGACAC
ACCTGTCGTCAAGTTTGTAAACAAAGTATTGCTGGATGCCATTAAGCGTGGTGCATCAGATATACATTTCGAACCCTATG
AAAGAACCTATCGTGTGCGTTATCGTATTGACGGGGTTCTGACAACTATGGCTAAGCCGCCTAAGAATATGACGTTGAAG
ATTTCTTCGCGTCTCAAGGTTATGGCTAATCTGGACATTGCCGAAAAAAGGGTGCCACAAGATGGTCGTGTTAAGCTGAA
TCTATCGAAAACCAATGCCATTGATTTTCGTATGAGTACATGCCCAACTCTTTTTGGTGAAAAAATTGTGATGCGTGTTT
TAGATGCATCGGCCGCCAAAATGGGTGTAGATAAGCTGGGTTATGAAGAGGAACAGAAAAGACTTTTTCTTGATGCCATT
CAAAAACCCTATGGAATGGTGTTGGTAACAGGCCCAACTGGTAGTGGTAAAACAGTATCGCTTTATACAGCTCTTAATAT
TCTGAATATTGAAGGCACCAACATTTCAACTGTAGAAGATCCGGTTGAAATTCGTGTTCATGGTATCAATCAGGTACAAC
AAAACACCAAGCAAGGTATGACTTTTGCAGCTGCATTGAGATCGTTCTTGCGTCAGGATCCGGACGTGATCATGGTTGGT
GAGATTCGTGATCTGGAAACAGCGGAAATTGCCATTAAGGCAGCGCAGACTGGTCATATGGTGTTGTCTACATTGCATAC
CAATAATGCGCCTGAGACGATCAGCCGCTTGATGAATATGGGCGTGGCGCCGTTCAACATTACTTCCGCCGTGGCTCTGG
TTGTCGCCCAGAGACTGGCAAGGCGTTTACATGATTGTAAAGCGGCGACAGAACTGCCAAAAGAAGCCTTGCTGGAAGCA
GGTATGAGCGAAGAAGATTTAGTCGATGCACAAATATTTGATGCAGTAGGTTGCCGTAAATGCAACCAAGGCTACAAAGC
ACGAGTGGGCATTTATGAAGTGATGCCTTTATCAACAAACATTAAGAAAATTATACTTAATAGTGGTAATGCACTGGATA
TTGCAGATCAAGCCCTCAAAGACGGCATTGCAGATTTACGTCAGTCTGCTTTGTTAAAAGTGAGAAATGGTATTACCAGT
TTGGTAGAAGCAAATAGAGTAACGGTAGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.34

100

0.549
  pilB Acinetobacter baylyi ADP1

53.996

98.772

0.533
  pilB Legionella pneumophila strain ERS1305867

53.721

96.667

0.519
  pilF Neisseria gonorrhoeae MS11

49.381

99.123

0.489
  pilB Vibrio cholerae strain A1552

49.905

92.807

0.463
  pilB Vibrio campbellii strain DS40M4

46.181

98.772

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

46.333

98.07

0.454
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.454

92.807

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

39.961

90.877

0.363

Multiple sequence alignment