Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACGB44_RS03480 Genome accession   NZ_CP171478
Coordinates   769848..771938 (+) Length   696 a.a.
NCBI ID   WP_316958970.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain RL-XB02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 764848..776938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGB44_RS03460 (ACGB44_03460) comM 767014..768078 (+) 1065 WP_110778198.1 pilus assembly protein PilM Machinery gene
  ACGB44_RS03465 (ACGB44_03465) - 768078..768650 (+) 573 WP_063539613.1 PilN domain-containing protein -
  ACGB44_RS03470 (ACGB44_03470) pilO 768647..769270 (+) 624 WP_200596014.1 type 4a pilus biogenesis protein PilO -
  ACGB44_RS03475 (ACGB44_03475) pilP 769267..769794 (+) 528 WP_125868795.1 type 4a pilus biogenesis lipoprotein PilP -
  ACGB44_RS03480 (ACGB44_03480) pilQ 769848..771938 (+) 2091 WP_316958970.1 type IV pilus secretin PilQ Machinery gene
  ACGB44_RS03485 (ACGB44_03485) aroK 771944..772489 (+) 546 WP_316958971.1 shikimate kinase AroK -
  ACGB44_RS03490 (ACGB44_03490) aroB 772512..773615 (+) 1104 WP_316958972.1 3-dehydroquinate synthase -
  ACGB44_RS03495 (ACGB44_03495) - 773626..775191 (+) 1566 WP_063539619.1 AAA family ATPase -

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 74884.50 Da        Isoelectric Point: 4.6189

>NTDB_id=1061386 ACGB44_RS03480 WP_316958970.1 769848..771938(+) (pilQ) [Stutzerimonas frequens strain RL-XB02]
MNSTLSRLGISMLVAFVSPAVLAANLNALDVAALPGDRVELKLAFDEPVAPPRGYTLEQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGKNLFVVLGENAGASGPVAVSAQPSAVAHPSVATAPAKAVTVGKA
IDNIDFRRGDDGAGNVIVTLSDPSISSTIEEQGGKIRVNFAKAQLPESLRVRLDVQDFATPVRFVDSASQAGGASIAIEP
TGRYDYLAYQTDNKLTISVKPLTEDEAEKRKADRFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVSGNITLRL
QNVPWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTNT
QGETEDRGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKALGVRWGGTQLFANGRGAVY
GNDDFGDEGGDSGEESSGNFPFVDMGVANRTAGIGIGYITDNLILDLELSAMEKSGNGEVVSQPKVMTADKETAKILKGS
EIPYQEASSSGATSTTFVEAALSLEVTPQITPDNRIIMEVKVTKDEPDFANDVNGTPTIRKNEVNAKILVNDGETVVIGG
VFSNTQSKSVDKVPFLGDLPYLGRMFRRDVVADSKSELLIFLTPRILNHQAVAVSR

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=1061386 ACGB44_RS03480 WP_316958970.1 769848..771938(+) (pilQ) [Stutzerimonas frequens strain RL-XB02]
ATGAACAGTACTCTCTCGCGTCTCGGCATTTCAATGCTTGTGGCGTTCGTTTCGCCGGCTGTGCTGGCAGCCAATCTCAA
TGCCTTGGATGTGGCCGCTCTGCCGGGCGATCGAGTCGAGCTCAAGCTGGCTTTCGACGAGCCCGTTGCGCCGCCGCGTG
GTTACACCCTTGAACAGCCTGCTCGTATTGCCCTGGATCTACCGGGTGTATCGAACAACCTGGGGGCGAAGAATCGTGAG
CTGGGGGTTGGTAATGCGCGTAGCGTTACGGTCGTTGAGGCGCAAGGACGTACTCGGTTGATCGTGAACCTCACTTCGCT
GGTGCCCTATTCCACTCGAGTGGATGGCAAGAACCTGTTCGTCGTGCTGGGCGAAAATGCCGGCGCGAGTGGCCCAGTAG
CGGTCTCAGCGCAGCCGAGCGCAGTGGCGCATCCATCTGTTGCGACGGCTCCCGCGAAGGCGGTGACTGTTGGTAAGGCC
ATTGACAACATTGACTTTCGGCGTGGCGATGATGGGGCTGGTAATGTGATTGTTACACTATCTGACCCATCAATCAGCTC
GACCATTGAGGAGCAGGGCGGAAAAATTCGCGTAAATTTCGCCAAGGCTCAGTTGCCGGAATCGCTGCGTGTGCGCCTGG
ATGTTCAAGACTTCGCTACGCCTGTCAGGTTCGTTGATTCCGCTTCTCAAGCAGGTGGTGCGAGCATCGCAATCGAGCCG
ACTGGTCGATATGACTATTTGGCCTATCAGACCGACAACAAGCTGACTATCAGCGTCAAACCTCTTACGGAAGACGAAGC
GGAAAAGCGTAAGGCTGATCGATTTGCTTATTCTGGCGAGAAGCTGTCGCTTAATTTCCAAGACATCGACGTTCGCTCGG
TATTGCAGCTAATCGCTGATTTCACCGATCTAAATCTGGTCGCAAGCGATACCGTTAGCGGCAATATCACGCTGCGTCTG
CAGAACGTGCCGTGGGATCAGGCTCTGGACCTGGTGCTCAAGACCAAGGGGTTGGACAAGCGCCAGGTCGGTAACGTGCT
ACTGGTCGCTCCTGCCGACGAAATTGCCGCACGTGAACGTCAGGAGCTTGAGTCTCAGCGGCAGATTGCCGAACTGGCTC
CGCTTCGCCGTGAGGTGGTGCAGGTTAACTACGCCAAGGCGGCGGATATCGCTCGCCTTTTCCAGTCTGTAACCAATACC
CAGGGCGAAACCGAGGACCGCGGTTCGATAGCCGTTGATGATAGAACCAACAATATTATTGCCTACCAGACGCAAGAGCG
TCTGGACGAGCTACGGCGGATCGTCGCGCAACTCGACATCCCTGTCCGGCAGGTCATGATCGAGGCTCGAATTGTTGAAG
CGAACGTGGATTATGATAAAGCGCTGGGCGTGCGCTGGGGCGGCACGCAGCTTTTTGCCAACGGGCGGGGCGCTGTCTAT
GGCAACGACGATTTTGGCGATGAAGGTGGAGACTCAGGGGAAGAAAGCAGCGGTAATTTTCCTTTCGTCGATATGGGCGT
CGCTAACCGTACTGCAGGTATCGGCATCGGTTACATTACCGACAACCTGATCCTTGACCTTGAGCTGTCGGCAATGGAGA
AGTCTGGTAACGGCGAGGTCGTGTCGCAGCCTAAGGTAATGACCGCTGACAAGGAAACCGCGAAGATCCTCAAGGGCTCT
GAGATTCCTTACCAAGAGGCCAGTTCAAGTGGCGCTACATCGACTACCTTTGTCGAGGCAGCTCTATCGCTGGAGGTTAC
GCCGCAGATCACTCCAGATAATCGCATCATCATGGAAGTCAAAGTGACCAAGGATGAGCCCGACTTTGCCAACGATGTGA
ACGGAACTCCTACCATTCGCAAAAACGAGGTGAATGCAAAGATTCTGGTGAATGACGGTGAGACAGTTGTCATCGGAGGC
GTCTTCTCCAACACCCAGAGCAAGTCAGTAGATAAAGTTCCTTTCTTGGGTGATCTGCCTTATCTTGGACGGATGTTCCG
CCGAGATGTTGTGGCGGACTCGAAGTCCGAATTGCTGATATTTCTGACTCCCAGAATACTCAATCATCAGGCCGTCGCTG
TAAGCCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(378-444)

Secretin and TonB N terminus short domain (STN)

(305-351)

Bacterial type II and III secretion system protein (Secretin)

(531-687)

AMIN domain (AMIN)

(38-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.905

100

0.743
  comQ Acinetobacter baylyi ADP1

41.643

99.713

0.415
  pilQ Acinetobacter baumannii strain A118

38.995

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.995

100

0.412

Multiple sequence alignment