Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACGK9W_RS09930 Genome accession   NZ_CP171359
Coordinates   2120107..2121822 (+) Length   571 a.a.
NCBI ID   WP_405420749.1    Uniprot ID   -
Organism   Marinobacter flavimaris strain ZYH30     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2097179..2119112 2120107..2121822 flank 995


Gene organization within MGE regions


Location: 2097179..2121822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGK9W_RS09820 (ACGK9W_09820) - 2097179..2098312 (-) 1134 WP_405420691.1 hypothetical protein -
  ACGK9W_RS09825 (ACGK9W_09825) - 2098392..2099465 (-) 1074 WP_405420694.1 glycosyltransferase -
  ACGK9W_RS09830 (ACGK9W_09830) - 2099537..2100121 (-) 585 WP_405420697.1 serine O-acetyltransferase -
  ACGK9W_RS09835 (ACGK9W_09835) - 2100225..2101592 (-) 1368 WP_405420700.1 hypothetical protein -
  ACGK9W_RS09840 (ACGK9W_09840) pseI 2101592..2102644 (-) 1053 WP_405420703.1 pseudaminic acid synthase -
  ACGK9W_RS09845 (ACGK9W_09845) pseH 2102648..2103160 (-) 513 WP_405420706.1 UDP-4-amino-4, 6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase -
  ACGK9W_RS09850 (ACGK9W_09850) - 2103157..2103573 (-) 417 WP_405420708.1 glycosyltransferase -
  ACGK9W_RS09855 (ACGK9W_09855) - 2103827..2104264 (-) 438 WP_405420711.1 hypothetical protein -
  ACGK9W_RS09860 (ACGK9W_09860) pseF 2104261..2104965 (-) 705 WP_405420714.1 pseudaminic acid cytidylyltransferase -
  ACGK9W_RS09865 (ACGK9W_09865) pseC 2104953..2106137 (-) 1185 WP_405420717.1 UDP-4-amino-4, 6-dideoxy-N-acetyl-beta-L-altrosamine transaminase -
  ACGK9W_RS09870 (ACGK9W_09870) pseB 2106140..2107138 (-) 999 WP_405421029.1 UDP-N-acetylglucosamine 4,6-dehydratase (inverting) -
  ACGK9W_RS09875 (ACGK9W_09875) - 2107147..2107785 (-) 639 WP_405420719.1 acyltransferase -
  ACGK9W_RS09880 (ACGK9W_09880) - 2107686..2108912 (-) 1227 WP_405420721.1 oligosaccharide flippase family protein -
  ACGK9W_RS09885 (ACGK9W_09885) - 2109015..2109980 (-) 966 WP_405420724.1 Wzz/FepE/Etk N-terminal domain-containing protein -
  ACGK9W_RS09890 (ACGK9W_09890) - 2109980..2112472 (-) 2493 WP_405420727.1 SLBB domain-containing protein -
  ACGK9W_RS09895 (ACGK9W_09895) - 2112590..2114071 (-) 1482 WP_405420730.1 capsule assembly Wzi family protein -
  ACGK9W_RS09900 (ACGK9W_09900) rfaH 2114305..2114784 (-) 480 WP_405420733.1 transcription/translation regulatory transformer protein RfaH -
  ACGK9W_RS09905 (ACGK9W_09905) cysQ 2114781..2115557 (-) 777 WP_405420735.1 3'(2'),5'-bisphosphate nucleotidase CysQ -
  ACGK9W_RS09910 (ACGK9W_09910) - 2115720..2117081 (+) 1362 WP_405420737.1 MBL fold metallo-hydrolase -
  ACGK9W_RS09915 (ACGK9W_09915) - 2117109..2117552 (-) 444 WP_405420740.1 pilin -
  ACGK9W_RS09920 (ACGK9W_09920) - 2117616..2119112 (-) 1497 WP_405420743.1 O-antigen ligase family protein -
  ACGK9W_RS09925 (ACGK9W_09925) - 2119205..2119678 (-) 474 WP_405420746.1 pilin -
  ACGK9W_RS09930 (ACGK9W_09930) pilB 2120107..2121822 (+) 1716 WP_405420749.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62899.96 Da        Isoelectric Point: 4.8170

>NTDB_id=1060866 ACGK9W_RS09930 WP_405420749.1 2120107..2121822(+) (pilB) [Marinobacter flavimaris strain ZYH30]
MSTNKSNITLTGLARRFVDDGLLDEATATEAFIQASQNRIPLITYLTQNSLADSSRLAFSAAMEFGVSVLDLSAFLPEMM
PEKVVDEKLVRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAVLVDDAKLREAIDKYLESQDTTMGDLDDS
DLEGVETEGGEQEDDGAVVASEVDDAPIVKYVNKMLLDAIRGGASDIHFEPYEKVYRVRYRTDGILKEVSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKQMYMDALK
QPQGMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPAEINLEGINQVNVNNRVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSLIIAQRLGRRLCSACKQPFEVPKDVLLEE
GFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAKQAQAEGFRNLRQSALMKVIEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1060866 ACGK9W_RS09930 WP_405420749.1 2120107..2121822(+) (pilB) [Marinobacter flavimaris strain ZYH30]
ATGTCTACTAACAAGAGCAACATCACGCTTACAGGCCTCGCCAGGCGATTCGTGGATGACGGTCTGCTGGATGAGGCGAC
AGCAACAGAAGCCTTTATCCAGGCGTCCCAGAATCGGATCCCTCTGATTACTTATCTGACCCAGAACAGTCTGGCAGACA
GTTCCAGACTCGCCTTTTCTGCGGCTATGGAATTTGGCGTCTCTGTTCTGGATTTGTCCGCGTTTCTTCCGGAAATGATG
CCGGAGAAGGTGGTTGACGAGAAGCTGGTCCGCAAGCATAACGCTCTGCCTCTTTATAAGAGAGGAAATAGGCTGTTCAT
TGCCGTTTCCGATCCAACTAACATCCAGGCGCTGGATGAAATCAAGTTCAACACGGGTTTAAGCACCGATGCGGTCCTAG
TAGATGATGCCAAACTTCGGGAGGCCATCGACAAATATCTGGAGTCCCAGGACACCACAATGGGTGATCTGGATGATTCC
GATCTTGAGGGGGTTGAGACTGAAGGAGGTGAGCAAGAAGATGATGGCGCTGTTGTCGCCAGTGAGGTGGACGACGCGCC
AATTGTAAAGTACGTGAATAAGATGCTTCTGGATGCGATTCGCGGCGGAGCCTCGGATATTCATTTCGAACCCTATGAGA
AAGTCTATCGGGTCCGATACCGCACAGACGGAATACTAAAGGAAGTCTCGCGACCCTCCATAAAGCTGGCTCCGAAGATT
TCCGCCCGGGTAAAGATCATGGCTCAGCTGGACATTTCTGAGCGGCGGGTACCTCAGGATGGCCGCATCAAGATGAAGCT
GTCCAAAACCAAGGCGATCGATTTCCGGGTAAACACCCTGCCCACTCTTTGGGGTGAAAAGATCGTTTTGCGAATTCTTG
ATCCCAGTCAGGCCAAACTAGGCATTGATGTACTCGGTTATGAGGAAGATCAGAAACAGATGTACATGGATGCCCTGAAA
CAGCCACAGGGCATGATTTTGGTGACTGGACCGACTGGCTCAGGCAAAACGGTCTCTTTGTATACCGGGCTGAATATTCT
GAATACCCCAGACATCAACATCTCCACTGCGGAAGACCCGGCGGAAATCAACCTGGAGGGCATTAACCAGGTAAACGTGA
ACAACCGTGTGGGGCTGGGCTTTGCCGAAGCCCTACGGGCATTCCTGCGCCAGGATCCCGATGTCATCATGGTCGGTGAG
ATACGGGACCTTGAAACGGCTAACATCGCTATCAAAGCGGCTCAGACTGGGCACCTGGTTCTTTCTACCCTTCACACGAA
CAGCGCTGCAGAAACCCTCACGCGGATGATGAACATGGGGGTGCCCTCGTTCAACATCGCTACCTCGGTAAGCCTGATTA
TTGCTCAGCGCCTAGGCCGGAGGCTGTGTAGCGCCTGCAAACAGCCCTTTGAAGTCCCAAAAGACGTGCTTTTGGAGGAA
GGGTTCACACAGGAACAAATTGATACAGGGTTCACATTGTACCGCCCCAAGGGCTGTGATAAATGCAATGGAGGATATAA
AGGCCGCGTCGGGATTTACGAAGTAGTGAAAATTACCGACGAACTGGCGAACATGATTATGGAAGAAGCCAGTTCCATCA
AAATTGCAAAGCAGGCTCAGGCTGAAGGCTTCCGTAACTTGCGCCAGTCAGCTCTCATGAAAGTGATTGAGGGCGTGACC
AGCCTTGAGGAAGCCAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.118

100

0.576
  pilB Acinetobacter baylyi ADP1

56.469

100

0.566
  pilB Legionella pneumophila strain ERS1305867

54.064

99.124

0.536
  pilB Vibrio parahaemolyticus RIMD 2210633

51.706

97.548

0.504
  pilB Vibrio campbellii strain DS40M4

50.709

98.774

0.501
  pilB Vibrio cholerae strain A1552

54.335

90.893

0.494
  pilF Neisseria gonorrhoeae MS11

47.957

98.599

0.473
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.304

92.119

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

40.462

90.893

0.368

Multiple sequence alignment