Detailed information NTG1060358

Bioinformatically predicted

Overview

• Name: pilO
• Type: Machinery gene
• Locus tag: ACFN7Z_RS01415
• Genome accession: NZ_CP171157
• Coordinates: 312651..313373 (+)
• Length: 240 a.a.
• NCBI ID: WP_004691497.1
• Uniprot ID: A0A380U8X3
• Organism: Acinetobacter sp. P1332
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 307651..318373

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFN7Z_RS01400 (ACFN7Z_01400)	ponA	308246..310774 (-)	2529	WP_395442452.1	penicillin-binding protein PBP1a	-
ACFN7Z_RS01405 (ACFN7Z_01405)	pilM	310940..311998 (+)	1059	WP_395442454.1	pilus assembly protein PilM	Machinery gene
ACFN7Z_RS01410 (ACFN7Z_01410)	pilN	311998..312654 (+)	657	WP_395442456.1	PilN domain-containing protein	Machinery gene
ACFN7Z_RS01415 (ACFN7Z_01415)	pilO	312651..313373 (+)	723	WP_004691497.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACFN7Z_RS01420 (ACFN7Z_01420)	pilP	313373..313900 (+)	528	WP_094148569.1	pilus assembly protein PilP	Machinery gene
ACFN7Z_RS01425 (ACFN7Z_01425)	pilQ	313920..315998 (+)	2079	WP_395442458.1	type IV pilus secretin PilQ	Machinery gene
ACFN7Z_RS01430 (ACFN7Z_01430)	-	316087..316569 (+)	483	WP_249303512.1	shikimate kinase	-
ACFN7Z_RS01435 (ACFN7Z_01435)	aroB	316600..317685 (+)	1086	WP_395442460.1	3-dehydroquinate synthase	-

Sequence

Protein

• Length: 240 a.a.
• Molecular weight: 27004.43 Da
• Isoelectric Point: 4.9937

>NTDB_id=1060358 ACFN7Z_RS01415 WP_004691497.1 312651..313373(+) (pilO) [Acinetobacter sp. P1332]
MSRDEFEELTSENHAVTKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNSGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNEKKTDIPQISYSVKAKTYRYVGATAAASVENTAKADAGGAQ

Nucleotide

• Length: 723 bp

>NTDB_id=1060358 ACFN7Z_RS01415 WP_004691497.1 312651..313373(+) (pilO) [Acinetobacter sp. P1332]
ATGAGTCGCGATGAATTTGAAGAATTAACATCAGAAAACCATGCTGTAACCAAGAAAAAAATGACGGTTGATAAATTTTT
TCAACAGTTTAATACCCTAGATCCAAACAATTACGGTAGTTGGCCGTTTTCAGTCAAATTGACTTGTTGGATCTTTATCA
TTTTAGTGATTGGGGCATTGGGTTATTTTGTCGTGATTAAGCCGAAGCTTGATGCGATTTCAAATGCACATGCACAAGAG
CAAAATTTACTGAATGAGTTTCGTGAAAAAGATTCAAAATTACGTAATTTGCAGCAATATCAAGCGCAATTGCAAGAAAT
GGAAGCCAATTTTAATCAGCAGTTAGAACAGCTGCCGAAAGAAACTGAAATTCCTGGCTTGGTTGAAGATATCAACGTGA
GCGGCGTAAATTCTGGATTAAAGTTTAAAAATATTCGTTTAGAACCAGAAATTCGCCAAGAGTTCTTTATCGAACAGCCG
ATTTCCATTGAAGCGACTGGTGATTATCATTCTTTTGGTTCATTTGTCAGTGGAATTGCAGCTTTGCCGCGTATTGTGAC
CTTGCATGACTTCGATATCTCAGCATCAAGTAATAATGAGAAAAAGACGGATATTCCGCAAATTTCATACAGTGTAAAGG
CTAAAACTTACCGCTATGTCGGGGCGACAGCTGCTGCAAGTGTGGAAAATACAGCTAAAGCAGATGCAGGAGGTGCTCAG
TAA

Domains

Predicted by InterproScan.

• Pilus assembly protein, PilO (PilO) (74-220)

3D structure

• AlphaFold DB: A0A380U8X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilO	Acinetobacter baumannii D1279779	72.385	99.583	0.721
comO	Acinetobacter baylyi ADP1	67.755	100	0.692