Detailed information NTG1058339

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACFXAB_RS10450
• Genome accession: NZ_CP170650
• Coordinates: 2055014..2055628 (+)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain JP5039
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2050014..2060628

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFXAB_RS10415 (ACFXAB_10415)	aroE	2050701..2051543 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
ACFXAB_RS10420 (ACFXAB_10420)	yhbY	2051537..2051827 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACFXAB_RS10425 (ACFXAB_10425)	-	2051838..2052407 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACFXAB_RS10430 (ACFXAB_10430)	yqeK	2052397..2052957 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACFXAB_RS10435 (ACFXAB_10435)	rsfS	2052975..2053331 (+)	357	WP_007408260.1	ribosome silencing factor	-
ACFXAB_RS10440 (ACFXAB_10440)	-	2053328..2054065 (+)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
ACFXAB_RS10445 (ACFXAB_10445)	comER	2054134..2054955 (-)	822	WP_014418420.1	late competence protein ComER	-
ACFXAB_RS10450 (ACFXAB_10450)	comEA	2055014..2055628 (+)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACFXAB_RS10455 (ACFXAB_10455)	-	2055695..2056264 (+)	570	WP_003152868.1	ComE operon protein 2	-
ACFXAB_RS10460 (ACFXAB_10460)	comEC	2056265..2058616 (+)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACFXAB_RS10465 (ACFXAB_10465)	-	2058635..2058769 (-)	135	WP_003152870.1	YqzM family protein	-
ACFXAB_RS10470 (ACFXAB_10470)	-	2058810..2058961 (+)	152	Protein_2002	hypothetical protein	-
ACFXAB_RS10475 (ACFXAB_10475)	holA	2059001..2060042 (+)	1042	Protein_2003	DNA polymerase III subunit delta	-
ACFXAB_RS10480 (ACFXAB_10480)	rpsT	2060059..2060325 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=1058339 ACFXAB_RS10450 WP_014418419.1 2055014..2055628(+) (comEA) [Bacillus velezensis strain JP5039]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1058339 ACFXAB_RS10450 WP_014418419.1 2055014..2055628(+) (comEA) [Bacillus velezensis strain JP5039]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363