Detailed information NTG1052330

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACEPM6_RS09190
• Genome accession: NZ_CP169570
• Coordinates: 1858996..1859610 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain CIMT3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1853996..1864610

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEPM6_RS09160 (ACEPM6_09160)	rpsT	1854299..1854565 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACEPM6_RS09165 (ACEPM6_09165)	holA	1854582..1855623 (-)	1042	Protein_1807	DNA polymerase III subunit delta	-
ACEPM6_RS09170 (ACEPM6_09170)	-	1855663..1855814 (-)	152	Protein_1808	hypothetical protein	-
ACEPM6_RS09175 (ACEPM6_09175)	-	1855855..1855989 (+)	135	WP_003152870.1	YqzM family protein	-
ACEPM6_RS09180 (ACEPM6_09180)	comEC	1856008..1858359 (-)	2352	WP_022552933.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACEPM6_RS09185 (ACEPM6_09185)	-	1858360..1858929 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACEPM6_RS09190 (ACEPM6_09190)	comEA	1858996..1859610 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACEPM6_RS09195 (ACEPM6_09195)	comER	1859669..1860490 (+)	822	WP_014418420.1	late competence protein ComER	-
ACEPM6_RS09200 (ACEPM6_09200)	-	1860559..1861296 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
ACEPM6_RS09205 (ACEPM6_09205)	rsfS	1861293..1861649 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACEPM6_RS09210 (ACEPM6_09210)	yqeK	1861667..1862227 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACEPM6_RS09215 (ACEPM6_09215)	-	1862217..1862786 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACEPM6_RS09220 (ACEPM6_09220)	yhbY	1862797..1863087 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACEPM6_RS09225 (ACEPM6_09225)	aroE	1863081..1863923 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=1052330 ACEPM6_RS09190 WP_014418419.1 1858996..1859610(-) (comEA) [Bacillus velezensis strain CIMT3]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1052330 ACEPM6_RS09190 WP_014418419.1 1858996..1859610(-) (comEA) [Bacillus velezensis strain CIMT3]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363