Detailed information NTG1052112

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACE17W_RS12350
• Genome accession: NZ_CP169562
• Coordinates: 2759336..2760400 (+)
• Length: 354 a.a.
• NCBI ID: WP_010946662.1
• Uniprot ID: Q5ZX06
• Organism: Legionella pneumophila strain Lp02
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2754336..2765400

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACE17W_RS12340 (ACE17W_12340)	-	2755373..2757757 (-)	2385	WP_010946660.1	penicillin-binding protein 1A	-
ACE17W_RS12345 (ACE17W_12345)	ravI	2757909..2758919 (-)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
ACE17W_RS12350 (ACE17W_12350)	pilM	2759336..2760400 (+)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
ACE17W_RS12355 (ACE17W_12355)	pilN	2760404..2760952 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
ACE17W_RS12360 (ACE17W_12360)	pilO	2760966..2761565 (+)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACE17W_RS12365 (ACE17W_12365)	pilP	2761562..2762149 (+)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
ACE17W_RS12370 (ACE17W_12370)	pilQ	2762153..2764249 (+)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
ACE17W_RS12375 (ACE17W_12375)	aroK	2764695..2765222 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38732.71 Da
• Isoelectric Point: 5.0367

>NTDB_id=1052112 ACE17W_RS12350 WP_010946662.1 2759336..2760400(+) (pilM) [Legionella pneumophila strain Lp02]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQSGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=1052112 ACE17W_RS12350 WP_010946662.1 2759336..2760400(+) (pilM) [Legionella pneumophila strain Lp02]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATCCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGCGAAGAAATACCTGCCTCTGCCTTAGAAGGAAATATAA
TTAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATCGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAATCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5ZX06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.435	100	0.994
comM	Acinetobacter nosocomialis M2	42.817	100	0.429
pilM	Acinetobacter baumannii D1279779	42.535	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418