Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEPA4_RS15735 Genome accession   NZ_CP169216
Coordinates   3401910..3403640 (-) Length   576 a.a.
NCBI ID   WP_164229951.1    Uniprot ID   -
Organism   Stenotrophomonas sp. P2112     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3396910..3408640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPA4_RS15720 (ACEPA4_15720) sucC 3397151..3398320 (-) 1170 WP_005418614.1 ADP-forming succinate--CoA ligase subunit beta -
  ACEPA4_RS15725 (ACEPA4_15725) - 3398728..3400341 (+) 1614 WP_061480577.1 PAS domain-containing sensor histidine kinase -
  ACEPA4_RS15730 (ACEPA4_15730) pilR 3400402..3401775 (+) 1374 WP_065178929.1 sigma-54 dependent transcriptional regulator Regulator
  ACEPA4_RS15735 (ACEPA4_15735) pilB 3401910..3403640 (-) 1731 WP_164229951.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEPA4_RS15740 (ACEPA4_15740) - 3404003..3404875 (+) 873 WP_164229953.1 glycosyltransferase family 2 protein -
  ACEPA4_RS15745 (ACEPA4_15745) - 3404887..3406101 (+) 1215 WP_219634362.1 glycosyltransferase family 4 protein -
  ACEPA4_RS15750 (ACEPA4_15750) - 3406145..3407341 (+) 1197 WP_235643011.1 lipopolysaccharide biosynthesis protein -
  ACEPA4_RS15755 (ACEPA4_15755) - 3407304..3408419 (-) 1116 WP_111202054.1 glycosyltransferase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62168.09 Da        Isoelectric Point: 5.8733

>NTDB_id=1049837 ACEPA4_RS15735 WP_164229951.1 3401910..3403640(-) (pilB) [Stenotrophomonas sp. P2112]
MNAVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGIGNLDVSGGDEDISGGGDAGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPANLPEH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKA
AAGITSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=1049837 ACEPA4_RS15735 WP_164229951.1 3401910..3403640(-) (pilB) [Stenotrophomonas sp. P2112]
ATGAACGCCGTAACCACCGCCAATCTCGTCGGCATCACCGGCTTGGCCCGTCGCCTGGTCCAGGATGGTGCGCTGGACGA
GGCTTCCGCCCGCGACGCCATGGCCAAGGCCGCTGCGGCCCGCCAGCCGCTGCCGACCTGGTTCGCGCAGAACAAGGTGG
TTGGGGCTGCTCAATTGGCGGCCGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGCGGCAAGCT
GTTCGTGGGCACCAGCAACCCGACCCATTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAGCCGATCCTGG
TGGATGAAGACCAGATCCGCCGCACGCTCGAGCAGTGGCATGCCAGTCATGACACGCTGGGCGACGCGCTGGGCGGTGAC
GACGATGGCATTGGTAACCTGGATGTGTCCGGCGGTGACGAGGACATTTCCGGGGGCGGCGACGCCGGCATCGATGCCAA
GGGCGATGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCAAGGGCGCCTCGGACATCCACT
TCGAGCCCTATGAAGATGACTACCGCGTGCGCCTGCGCATCGATGGCCTACTGAAGATGGTCGCGCGCGCGCCGGTGAAG
CTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCACAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGACG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGTACGCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTC
CTGGAAGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGTAAGACCGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCCCTGCGCTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTC
CACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCT
CGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGGCTGTGCAGCAACTGCAAGCGCCCGGCCAATCTGCCCGAGCAT
GCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGACGCGGGTATCGAGCTGTACGAGGCAGTCGGCTGCGATGAGTG
CACCGAGGGCTACAAGGGTCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGCCACGATCGTGCTGGCTG
GCGGCAATGCGTTGCAGATTGCCGAGGCCGCACAGCAGATCGGGGTGAACGATCTGCGCCAGTCGGCGTTGAAGAAGGCC
GCTGCGGGCATCACCAGCCTGGCCGAGATCAACCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.44

100

0.557
  pilB Acinetobacter baylyi ADP1

54.795

100

0.556
  pilB Legionella pneumophila strain ERS1305867

51.923

99.306

0.516
  pilF Neisseria gonorrhoeae MS11

49.296

98.611

0.486
  pilB Vibrio cholerae strain A1552

48.427

99.306

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

44.965

100

0.45
  pilB Vibrio campbellii strain DS40M4

45.343

98.785

0.448

Multiple sequence alignment