Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACEQ85_RS09545 Genome accession   NZ_CP168966
Coordinates   2009792..2010544 (+) Length   250 a.a.
NCBI ID   WP_060464993.1    Uniprot ID   A0A1E2YH36
Organism   Acinetobacter pittii strain LS-AP     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2004792..2015544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEQ85_RS09530 (ACEQ85_09530) ponA 2005373..2007934 (-) 2562 WP_373882521.1 penicillin-binding protein PBP1a -
  ACEQ85_RS09535 (ACEQ85_09535) pilM 2008096..2009154 (+) 1059 WP_373882522.1 pilus assembly protein PilM Machinery gene
  ACEQ85_RS09540 (ACEQ85_09540) pilN 2009154..2009795 (+) 642 WP_033848908.1 PilN domain-containing protein Machinery gene
  ACEQ85_RS09545 (ACEQ85_09545) pilO 2009792..2010544 (+) 753 WP_060464993.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACEQ85_RS09550 (ACEQ85_09550) pilP 2010555..2011082 (+) 528 WP_068563956.1 pilus assembly protein PilP Machinery gene
  ACEQ85_RS09555 (ACEQ85_09555) pilQ 2011145..2013271 (+) 2127 WP_068563954.1 type IV pilus secretin PilQ Machinery gene
  ACEQ85_RS09560 (ACEQ85_09560) aroK 2013283..2013852 (+) 570 WP_002116987.1 shikimate kinase AroK -
  ACEQ85_RS09565 (ACEQ85_09565) aroB 2013881..2014963 (+) 1083 WP_044103315.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28241.10 Da        Isoelectric Point: 4.8782

>NTDB_id=1048990 ACEQ85_RS09545 WP_060464993.1 2009792..2010544(+) (pilO) [Acinetobacter pittii strain LS-AP]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFIVEAAPVKDGKSDIPVLNYSIKAKTYRYMGAVENPEHQEKNASASSVAP
DSSTANTQPK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=1048990 ACEQ85_RS09545 WP_060464993.1 2009792..2010544(+) (pilO) [Acinetobacter pittii strain LS-AP]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTTCT
TCAGCAATTTAACACGCTCGATATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTATTGGCTTTGGGGTATTTTGTTGCTATTCAACCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAACTTCAAGAAAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCTGGTCTGAAGTTTAAAAATATCCGCTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCT
ATTACGATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
GATGCATGATTTTATTGTTGAAGCTGCTCCTGTTAAGGATGGTAAATCAGATATTCCTGTGCTTAATTATTCTATCAAAG
CAAAAACTTATCGCTATATGGGTGCTGTAGAAAATCCTGAACATCAGGAAAAAAATGCTTCGGCTTCCTCGGTAGCACCT
GATTCTTCTACTGCTAACACACAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E2YH36

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.6

100

0.836
  comO Acinetobacter baylyi ADP1

69.583

96

0.668

Multiple sequence alignment